Evaluation of Antimicrobial Properties and Potential Applications of Pseudomonas gessardii M15 Rhamnolipids towards Multiresistant Staphylococcus aureus
Staphylococcus aureus is a Gram-positive opportunistic human pathogen responsible for severe infections and thousands of deaths annually, mostly due to its multidrug-resistant (MDR) variants. The cell membrane has emerged as a promising new therapeutic target, and lipophilic molecules, such as biosu...
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MDPI AG
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ftdoajarticles:oai:doaj.org/article:06356107973c46b49c4f94adb61f1280 2023-05-15T14:03:42+02:00 Evaluation of Antimicrobial Properties and Potential Applications of Pseudomonas gessardii M15 Rhamnolipids towards Multiresistant Staphylococcus aureus Carmine Buonocore Rosa Giugliano Gerardo Della Sala Fortunato Palma Esposito Pietro Tedesco Veronica Folliero Massimiliano Galdiero Gianluigi Franci Donatella de Pascale 2023-02-01T00:00:00Z https://doi.org/10.3390/pharmaceutics15020700 https://doaj.org/article/06356107973c46b49c4f94adb61f1280 EN eng MDPI AG https://www.mdpi.com/1999-4923/15/2/700 https://doaj.org/toc/1999-4923 doi:10.3390/pharmaceutics15020700 1999-4923 https://doaj.org/article/06356107973c46b49c4f94adb61f1280 Pharmaceutics, Vol 15, Iss 700, p 700 (2023) rhamnolipids biosurfactants MRSA S. aureus biofilm SEM Pharmacy and materia medica RS1-441 article 2023 ftdoajarticles https://doi.org/10.3390/pharmaceutics15020700 2023-02-26T01:28:52Z Staphylococcus aureus is a Gram-positive opportunistic human pathogen responsible for severe infections and thousands of deaths annually, mostly due to its multidrug-resistant (MDR) variants. The cell membrane has emerged as a promising new therapeutic target, and lipophilic molecules, such as biosurfactants, are currently being utilized. Herein, we evaluated the antimicrobial activity of a rhamnolipids mixture produced by the Antarctic marine bacterium Pseudomonas gessardii M15. We demonstrated that our mixture has bactericidal activity in the range of 12.5–50 µg/mL against a panel of clinical MDR isolates of S. aureus , and that the mixture eradicated the bacterial population in 30 min at MIC value, and in 5 min after doubling the concentration. We also tested abilities of RLs to interfere with biofilm at different stages and determined that RLs can penetrate biofilm and kill the bacteria at sub-MICs values. The mixture was then used to functionalize a cotton swab to evaluate the prevention of S. aureus proliferation. We showed that by using 8 µg of rhamnolipids per swab, the entire bacterial load is eradicated, and just 0.5 µg is sufficient to reduce the growth by 99.99%. Our results strongly indicate the possibility of using this mixture as an additive for wound dressings for chronic wounds. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Pharmaceutics 15 2 700 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
rhamnolipids biosurfactants MRSA S. aureus biofilm SEM Pharmacy and materia medica RS1-441 |
spellingShingle |
rhamnolipids biosurfactants MRSA S. aureus biofilm SEM Pharmacy and materia medica RS1-441 Carmine Buonocore Rosa Giugliano Gerardo Della Sala Fortunato Palma Esposito Pietro Tedesco Veronica Folliero Massimiliano Galdiero Gianluigi Franci Donatella de Pascale Evaluation of Antimicrobial Properties and Potential Applications of Pseudomonas gessardii M15 Rhamnolipids towards Multiresistant Staphylococcus aureus |
topic_facet |
rhamnolipids biosurfactants MRSA S. aureus biofilm SEM Pharmacy and materia medica RS1-441 |
description |
Staphylococcus aureus is a Gram-positive opportunistic human pathogen responsible for severe infections and thousands of deaths annually, mostly due to its multidrug-resistant (MDR) variants. The cell membrane has emerged as a promising new therapeutic target, and lipophilic molecules, such as biosurfactants, are currently being utilized. Herein, we evaluated the antimicrobial activity of a rhamnolipids mixture produced by the Antarctic marine bacterium Pseudomonas gessardii M15. We demonstrated that our mixture has bactericidal activity in the range of 12.5–50 µg/mL against a panel of clinical MDR isolates of S. aureus , and that the mixture eradicated the bacterial population in 30 min at MIC value, and in 5 min after doubling the concentration. We also tested abilities of RLs to interfere with biofilm at different stages and determined that RLs can penetrate biofilm and kill the bacteria at sub-MICs values. The mixture was then used to functionalize a cotton swab to evaluate the prevention of S. aureus proliferation. We showed that by using 8 µg of rhamnolipids per swab, the entire bacterial load is eradicated, and just 0.5 µg is sufficient to reduce the growth by 99.99%. Our results strongly indicate the possibility of using this mixture as an additive for wound dressings for chronic wounds. |
format |
Article in Journal/Newspaper |
author |
Carmine Buonocore Rosa Giugliano Gerardo Della Sala Fortunato Palma Esposito Pietro Tedesco Veronica Folliero Massimiliano Galdiero Gianluigi Franci Donatella de Pascale |
author_facet |
Carmine Buonocore Rosa Giugliano Gerardo Della Sala Fortunato Palma Esposito Pietro Tedesco Veronica Folliero Massimiliano Galdiero Gianluigi Franci Donatella de Pascale |
author_sort |
Carmine Buonocore |
title |
Evaluation of Antimicrobial Properties and Potential Applications of Pseudomonas gessardii M15 Rhamnolipids towards Multiresistant Staphylococcus aureus |
title_short |
Evaluation of Antimicrobial Properties and Potential Applications of Pseudomonas gessardii M15 Rhamnolipids towards Multiresistant Staphylococcus aureus |
title_full |
Evaluation of Antimicrobial Properties and Potential Applications of Pseudomonas gessardii M15 Rhamnolipids towards Multiresistant Staphylococcus aureus |
title_fullStr |
Evaluation of Antimicrobial Properties and Potential Applications of Pseudomonas gessardii M15 Rhamnolipids towards Multiresistant Staphylococcus aureus |
title_full_unstemmed |
Evaluation of Antimicrobial Properties and Potential Applications of Pseudomonas gessardii M15 Rhamnolipids towards Multiresistant Staphylococcus aureus |
title_sort |
evaluation of antimicrobial properties and potential applications of pseudomonas gessardii m15 rhamnolipids towards multiresistant staphylococcus aureus |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/pharmaceutics15020700 https://doaj.org/article/06356107973c46b49c4f94adb61f1280 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Pharmaceutics, Vol 15, Iss 700, p 700 (2023) |
op_relation |
https://www.mdpi.com/1999-4923/15/2/700 https://doaj.org/toc/1999-4923 doi:10.3390/pharmaceutics15020700 1999-4923 https://doaj.org/article/06356107973c46b49c4f94adb61f1280 |
op_doi |
https://doi.org/10.3390/pharmaceutics15020700 |
container_title |
Pharmaceutics |
container_volume |
15 |
container_issue |
2 |
container_start_page |
700 |
_version_ |
1766274506305830912 |