Differential response of digesta- and mucosa-associated intestinal microbiota to dietary insect meal during the seawater phase of Atlantic salmon
Abstract Background Intestinal digesta is commonly used for studying responses of microbiota to dietary shifts, yet evidence is accumulating that it represents an incomplete view of the intestinal microbiota. The present work aims to investigate the differences between digesta- and mucosa-associated...
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ftdoajarticles:oai:doaj.org/article:0621a1751fb7463abc8c92cb1865e1db 2023-05-15T15:30:41+02:00 Differential response of digesta- and mucosa-associated intestinal microbiota to dietary insect meal during the seawater phase of Atlantic salmon Yanxian Li Leonardo Bruni Alexander Jaramillo-Torres Karina Gajardo Trond M. Kortner Åshild Krogdahl 2021-01-01T00:00:00Z https://doi.org/10.1186/s42523-020-00071-3 https://doaj.org/article/0621a1751fb7463abc8c92cb1865e1db EN eng BMC https://doi.org/10.1186/s42523-020-00071-3 https://doaj.org/toc/2524-4671 doi:10.1186/s42523-020-00071-3 2524-4671 https://doaj.org/article/0621a1751fb7463abc8c92cb1865e1db Animal Microbiome, Vol 3, Iss 1, Pp 1-18 (2021) Atlantic salmon Diet Black soldier fly Microbiota Digesta Mucosa Veterinary medicine SF600-1100 Microbiology QR1-502 article 2021 ftdoajarticles https://doi.org/10.1186/s42523-020-00071-3 2022-12-31T05:40:11Z Abstract Background Intestinal digesta is commonly used for studying responses of microbiota to dietary shifts, yet evidence is accumulating that it represents an incomplete view of the intestinal microbiota. The present work aims to investigate the differences between digesta- and mucosa-associated intestinal microbiota in Atlantic salmon (Salmo salar) and how they may respond differently to dietary perturbations. In a 16-week seawater feeding trial, Atlantic salmon were fed either a commercially-relevant reference diet or an insect meal diet containing ~ 15% black soldier fly (Hermetia illucens) larvae meal. The digesta- and mucosa-associated distal intestinal microbiota were profiled by 16S rRNA gene sequencing. Results Regardless of diet, we observed substantial differences between digesta- and mucosa-associated intestinal microbiota. Microbial richness and diversity were much higher in the digesta than the mucosa. The insect meal diet altered the distal intestinal microbiota resulting in higher microbial richness and diversity. The diet effect, however, depended on the sample origin. Digesta-associated intestinal microbiota showed more pronounced changes than the mucosa-associated microbiota. Multivariate association analyses identified two mucosa-enriched taxa, Brevinema andersonii and Spirochaetaceae, associated with the expression of genes related to immune responses and barrier function in the distal intestine, respectively. Conclusions Our data show that salmon intestinal digesta and mucosa harbor microbial communities with clear differences. While feeding insects increased microbial richness and diversity in both digesta- and mucosa-associated intestinal microbiota, mucosa-associated intestinal microbiota seems more resilient to variations in the diet composition. To fully unveil the response of intestinal microbiota to dietary changes, concurrent profiling of digesta- and mucosa-associated intestinal microbiota is recommended whenever feasible. Specific taxa enriched in the intestinal mucosa are ... Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Animal Microbiome 3 1 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Atlantic salmon Diet Black soldier fly Microbiota Digesta Mucosa Veterinary medicine SF600-1100 Microbiology QR1-502 |
spellingShingle |
Atlantic salmon Diet Black soldier fly Microbiota Digesta Mucosa Veterinary medicine SF600-1100 Microbiology QR1-502 Yanxian Li Leonardo Bruni Alexander Jaramillo-Torres Karina Gajardo Trond M. Kortner Åshild Krogdahl Differential response of digesta- and mucosa-associated intestinal microbiota to dietary insect meal during the seawater phase of Atlantic salmon |
topic_facet |
Atlantic salmon Diet Black soldier fly Microbiota Digesta Mucosa Veterinary medicine SF600-1100 Microbiology QR1-502 |
description |
Abstract Background Intestinal digesta is commonly used for studying responses of microbiota to dietary shifts, yet evidence is accumulating that it represents an incomplete view of the intestinal microbiota. The present work aims to investigate the differences between digesta- and mucosa-associated intestinal microbiota in Atlantic salmon (Salmo salar) and how they may respond differently to dietary perturbations. In a 16-week seawater feeding trial, Atlantic salmon were fed either a commercially-relevant reference diet or an insect meal diet containing ~ 15% black soldier fly (Hermetia illucens) larvae meal. The digesta- and mucosa-associated distal intestinal microbiota were profiled by 16S rRNA gene sequencing. Results Regardless of diet, we observed substantial differences between digesta- and mucosa-associated intestinal microbiota. Microbial richness and diversity were much higher in the digesta than the mucosa. The insect meal diet altered the distal intestinal microbiota resulting in higher microbial richness and diversity. The diet effect, however, depended on the sample origin. Digesta-associated intestinal microbiota showed more pronounced changes than the mucosa-associated microbiota. Multivariate association analyses identified two mucosa-enriched taxa, Brevinema andersonii and Spirochaetaceae, associated with the expression of genes related to immune responses and barrier function in the distal intestine, respectively. Conclusions Our data show that salmon intestinal digesta and mucosa harbor microbial communities with clear differences. While feeding insects increased microbial richness and diversity in both digesta- and mucosa-associated intestinal microbiota, mucosa-associated intestinal microbiota seems more resilient to variations in the diet composition. To fully unveil the response of intestinal microbiota to dietary changes, concurrent profiling of digesta- and mucosa-associated intestinal microbiota is recommended whenever feasible. Specific taxa enriched in the intestinal mucosa are ... |
format |
Article in Journal/Newspaper |
author |
Yanxian Li Leonardo Bruni Alexander Jaramillo-Torres Karina Gajardo Trond M. Kortner Åshild Krogdahl |
author_facet |
Yanxian Li Leonardo Bruni Alexander Jaramillo-Torres Karina Gajardo Trond M. Kortner Åshild Krogdahl |
author_sort |
Yanxian Li |
title |
Differential response of digesta- and mucosa-associated intestinal microbiota to dietary insect meal during the seawater phase of Atlantic salmon |
title_short |
Differential response of digesta- and mucosa-associated intestinal microbiota to dietary insect meal during the seawater phase of Atlantic salmon |
title_full |
Differential response of digesta- and mucosa-associated intestinal microbiota to dietary insect meal during the seawater phase of Atlantic salmon |
title_fullStr |
Differential response of digesta- and mucosa-associated intestinal microbiota to dietary insect meal during the seawater phase of Atlantic salmon |
title_full_unstemmed |
Differential response of digesta- and mucosa-associated intestinal microbiota to dietary insect meal during the seawater phase of Atlantic salmon |
title_sort |
differential response of digesta- and mucosa-associated intestinal microbiota to dietary insect meal during the seawater phase of atlantic salmon |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s42523-020-00071-3 https://doaj.org/article/0621a1751fb7463abc8c92cb1865e1db |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Animal Microbiome, Vol 3, Iss 1, Pp 1-18 (2021) |
op_relation |
https://doi.org/10.1186/s42523-020-00071-3 https://doaj.org/toc/2524-4671 doi:10.1186/s42523-020-00071-3 2524-4671 https://doaj.org/article/0621a1751fb7463abc8c92cb1865e1db |
op_doi |
https://doi.org/10.1186/s42523-020-00071-3 |
container_title |
Animal Microbiome |
container_volume |
3 |
container_issue |
1 |
_version_ |
1766361143292461056 |