An optimized one-tube, semi-nested PCR assay for Paracoccidioides brasiliensis detection

• Published in: Revista da Sociedade Brasileira de Medicina Tropical
• Main Authors: Amanda de Faveri Pitz, Andrea Cristine Koishi, Eliandro Reis Tavares, Fabio Goulart de Andrade, Eduardo Alexandre Loth, Rinaldo Ferreira Gandra, Emerson Jose Venancio
• Format: Article in Journal/Newspaper
• Language: English
• Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2013
• Subjects: Mycosis; Molecular diagnosis; Polymerase chain reaction assays; Arctic medicine. Tropical medicine; RC955-962; Arctic
• Online Access: https://doi.org/10.1590/0037-8682-1625-2013; https://doaj.org/article/0609c5a095a4426bb24255020c38e47e

Introduction Herein, we report a one-tube, semi-nested-polymerase chain reaction (OTsn-PCR) assay for the detection of Paracoccidioides brasiliensis. Methods We developed the OTsn-PCR assay for the detection of P. brasiliensis in clinical specimens and compared it with other PCR methods. Results The OTsn-PCR assay was positive for all clinical samples, and the detection limit was better or equivalent to the other nested or semi-nested PCR methods for P. brasiliensis detection. Conclusions The OTsn-PCR assay described in this paper has a detection limit similar to other reactions for the molecular detection of P. brasiliensis, but this approach is faster and less prone to contamination than other conventional nested or semi-nested PCR assays.