Evaluation of Immune Protection of a Bivalent Inactivated Vaccine against Aeromonas salmonicida and Vibrio vulnificus in Turbot
The Aeromonas salmonicida is responsible for causing furunculosis in various fish species. Furunculosis is a ubiquitous disease that affects the aquaculture industry and causes the mass mortality of turbot. Vibrio vulnificus is a pathogen that causes skin ulcers and hemorrhagic septicemia in fish, r...
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ftdoajarticles:oai:doaj.org/article:05f20bf2df7149c1bc9cf026713b8449 2024-09-09T20:12:30+00:00 Evaluation of Immune Protection of a Bivalent Inactivated Vaccine against Aeromonas salmonicida and Vibrio vulnificus in Turbot Yunji Xiu Jingyuan Yi Ruixin Feng Jiaxue Song Yunfei Pang Peng Liu Shun Zhou 2024-04-01T00:00:00Z https://doi.org/10.3390/fishes9040131 https://doaj.org/article/05f20bf2df7149c1bc9cf026713b8449 EN eng MDPI AG https://www.mdpi.com/2410-3888/9/4/131 https://doaj.org/toc/2410-3888 doi:10.3390/fishes9040131 2410-3888 https://doaj.org/article/05f20bf2df7149c1bc9cf026713b8449 Fishes, Vol 9, Iss 4, p 131 (2024) Aeromonas salmonicida Vibrio vulnificus bivalent inactivated vaccine RPS turbot Biology (General) QH301-705.5 Genetics QH426-470 article 2024 ftdoajarticles https://doi.org/10.3390/fishes9040131 2024-08-05T17:49:32Z The Aeromonas salmonicida is responsible for causing furunculosis in various fish species. Furunculosis is a ubiquitous disease that affects the aquaculture industry and causes the mass mortality of turbot. Vibrio vulnificus is a pathogen that causes skin ulcers and hemorrhagic septicemia in fish, resulting in significant mortality in aquaculture. In this study, we have established a bivalent inactivated vaccine against A. salmonicida and V. vulnificus with Montanideā¢ ISA 763 AVG as an adjuvant. This bivalent inactivated vaccine was used to immunize turbot by intraperitoneal injection, and the relevant immune indexes were detected. The results demonstrate that the bivalent inactivated vaccine exhibited a relative percent survival (RPS) of 77% following A. salmonicida and V. vulnificus intraperitoneal challenge. The vaccinated group exhibited higher levels of acid phosphatase activity and lysozyme activity compared to the control group. ELISA results showed a significant increase in serum antibody levels in immunized turbot, which was positively correlated with immunity. In the kidney tissue, related immune genes ( TLR5 , CD4 , MHCI and MHCII ) were up-regulated significantly, showing that the vaccine can induce cellular and humoral immune responses in turbot. In conclusion, the bivalent inactivated vaccine against A. salmonicida and V. vulnificus was immunogenic, efficiently preventing turbot from infection, which has the potential to be applied in aquaculture. Article in Journal/Newspaper Turbot Directory of Open Access Journals: DOAJ Articles Fishes 9 4 131 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Aeromonas salmonicida Vibrio vulnificus bivalent inactivated vaccine RPS turbot Biology (General) QH301-705.5 Genetics QH426-470 |
spellingShingle |
Aeromonas salmonicida Vibrio vulnificus bivalent inactivated vaccine RPS turbot Biology (General) QH301-705.5 Genetics QH426-470 Yunji Xiu Jingyuan Yi Ruixin Feng Jiaxue Song Yunfei Pang Peng Liu Shun Zhou Evaluation of Immune Protection of a Bivalent Inactivated Vaccine against Aeromonas salmonicida and Vibrio vulnificus in Turbot |
topic_facet |
Aeromonas salmonicida Vibrio vulnificus bivalent inactivated vaccine RPS turbot Biology (General) QH301-705.5 Genetics QH426-470 |
description |
The Aeromonas salmonicida is responsible for causing furunculosis in various fish species. Furunculosis is a ubiquitous disease that affects the aquaculture industry and causes the mass mortality of turbot. Vibrio vulnificus is a pathogen that causes skin ulcers and hemorrhagic septicemia in fish, resulting in significant mortality in aquaculture. In this study, we have established a bivalent inactivated vaccine against A. salmonicida and V. vulnificus with Montanideā¢ ISA 763 AVG as an adjuvant. This bivalent inactivated vaccine was used to immunize turbot by intraperitoneal injection, and the relevant immune indexes were detected. The results demonstrate that the bivalent inactivated vaccine exhibited a relative percent survival (RPS) of 77% following A. salmonicida and V. vulnificus intraperitoneal challenge. The vaccinated group exhibited higher levels of acid phosphatase activity and lysozyme activity compared to the control group. ELISA results showed a significant increase in serum antibody levels in immunized turbot, which was positively correlated with immunity. In the kidney tissue, related immune genes ( TLR5 , CD4 , MHCI and MHCII ) were up-regulated significantly, showing that the vaccine can induce cellular and humoral immune responses in turbot. In conclusion, the bivalent inactivated vaccine against A. salmonicida and V. vulnificus was immunogenic, efficiently preventing turbot from infection, which has the potential to be applied in aquaculture. |
format |
Article in Journal/Newspaper |
author |
Yunji Xiu Jingyuan Yi Ruixin Feng Jiaxue Song Yunfei Pang Peng Liu Shun Zhou |
author_facet |
Yunji Xiu Jingyuan Yi Ruixin Feng Jiaxue Song Yunfei Pang Peng Liu Shun Zhou |
author_sort |
Yunji Xiu |
title |
Evaluation of Immune Protection of a Bivalent Inactivated Vaccine against Aeromonas salmonicida and Vibrio vulnificus in Turbot |
title_short |
Evaluation of Immune Protection of a Bivalent Inactivated Vaccine against Aeromonas salmonicida and Vibrio vulnificus in Turbot |
title_full |
Evaluation of Immune Protection of a Bivalent Inactivated Vaccine against Aeromonas salmonicida and Vibrio vulnificus in Turbot |
title_fullStr |
Evaluation of Immune Protection of a Bivalent Inactivated Vaccine against Aeromonas salmonicida and Vibrio vulnificus in Turbot |
title_full_unstemmed |
Evaluation of Immune Protection of a Bivalent Inactivated Vaccine against Aeromonas salmonicida and Vibrio vulnificus in Turbot |
title_sort |
evaluation of immune protection of a bivalent inactivated vaccine against aeromonas salmonicida and vibrio vulnificus in turbot |
publisher |
MDPI AG |
publishDate |
2024 |
url |
https://doi.org/10.3390/fishes9040131 https://doaj.org/article/05f20bf2df7149c1bc9cf026713b8449 |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
Fishes, Vol 9, Iss 4, p 131 (2024) |
op_relation |
https://www.mdpi.com/2410-3888/9/4/131 https://doaj.org/toc/2410-3888 doi:10.3390/fishes9040131 2410-3888 https://doaj.org/article/05f20bf2df7149c1bc9cf026713b8449 |
op_doi |
https://doi.org/10.3390/fishes9040131 |
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Fishes |
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9 |
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131 |
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1809947085738344448 |