Expression patterns of molecular chaperone genes in Antarctic psychrophilic yeast, Glaciozyma antarctica PI12 in response to heat stress
Microbes living in the polar regions have some common and unique strategies to respond to thermal stress. Nevertheless, the amount of information available, especially at the molecular level is lacking for some organisms such as Antarctic psychrophilic yeast. For instance, it is not known whether mo...
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ftdoajarticles:oai:doaj.org/article:05e8461d7f9e47038e410466140703d3 2023-05-15T13:47:14+02:00 Expression patterns of molecular chaperone genes in Antarctic psychrophilic yeast, Glaciozyma antarctica PI12 in response to heat stress Nur Athirah Yusof Clemente Michael Vui Ling Wong Abdul Munir Abdul Murad Farah Diba Abu Bakar Nor Muhammad Mahadi Ahmad Yamin Abdul Rahman Nursyafiqi Zainuddin Mohd Nazalan Mohd Najimudin 2019-09-01T00:00:00Z https://doi.org/10.24425/ppr.2019.129674 https://doaj.org/article/05e8461d7f9e47038e410466140703d3 EN eng Polish Academy of Sciences https://journals.pan.pl/Content/113185/PDF/PPR%203-19%205-N.Yusof.pdf https://doaj.org/toc/0138-0338 https://doaj.org/toc/2081-8262 0138-0338 2081-8262 https://doi.org/10.24425/ppr.2019.129674 https://doaj.org/article/05e8461d7f9e47038e410466140703d3 Polish Polar Research, Vol vol. 40, Iss No 3, Pp 273-292 (2019) antarctic psychrophile molecular chaperone heat-stress transcriptome Geology QE1-996.5 article 2019 ftdoajarticles https://doi.org/10.24425/ppr.2019.129674 2022-12-30T22:45:28Z Microbes living in the polar regions have some common and unique strategies to respond to thermal stress. Nevertheless, the amount of information available, especially at the molecular level is lacking for some organisms such as Antarctic psychrophilic yeast. For instance, it is not known whether molecular chaperones in Antarctic yeasts play similar roles to those from mesophilic yeasts when they are exposed to heat stress. Therefore, this project aimed to determine the gene expression patterns and roles of molecular chaperones in Antarctic psychrophilic Glaciozyma antarctica PI12 that was exposed to heat stress. G. antarctica PI12 was grown at its optimal growth temperature of 12ºC and later exposed to heat stresses at 16ºC and 20ºC for 6 hours. Transcriptomes of those cells were extracted, sequenced and analyzed. Thirty-three molecular chaperone genes demonstrated differential expression of which 23 were up-regulated while 10 were down-regulated. Functions of up-regulated molecular chaperone genes were related to protein binding, response to a stimulus, chaperone binding, cellular response to stress, oxidation, and reduction, ATP binding, DNA-damage response and regulation for cellular protein metabolic process. On the other hand, functions of down-regulated molecular chaperone genes were related to chaperone-mediated protein complex assembly, transcription, cellular macromolecule metabolic process, regulation of cell growth and ribosome biogenesis. The findings provided information on how molecular chaperones work together in a complex network to protect the cells under heat stress. It also highlights the evolutionary conserved protective role of molecular chaperones in psychrophilic yeast, G. antarctica, and mesophilic yeast, Saccharomyces cerevisiae. Article in Journal/Newspaper Antarc* Antarctic Antarctica Polar Research Directory of Open Access Journals: DOAJ Articles Antarctic |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
antarctic psychrophile molecular chaperone heat-stress transcriptome Geology QE1-996.5 |
spellingShingle |
antarctic psychrophile molecular chaperone heat-stress transcriptome Geology QE1-996.5 Nur Athirah Yusof Clemente Michael Vui Ling Wong Abdul Munir Abdul Murad Farah Diba Abu Bakar Nor Muhammad Mahadi Ahmad Yamin Abdul Rahman Nursyafiqi Zainuddin Mohd Nazalan Mohd Najimudin Expression patterns of molecular chaperone genes in Antarctic psychrophilic yeast, Glaciozyma antarctica PI12 in response to heat stress |
topic_facet |
antarctic psychrophile molecular chaperone heat-stress transcriptome Geology QE1-996.5 |
description |
Microbes living in the polar regions have some common and unique strategies to respond to thermal stress. Nevertheless, the amount of information available, especially at the molecular level is lacking for some organisms such as Antarctic psychrophilic yeast. For instance, it is not known whether molecular chaperones in Antarctic yeasts play similar roles to those from mesophilic yeasts when they are exposed to heat stress. Therefore, this project aimed to determine the gene expression patterns and roles of molecular chaperones in Antarctic psychrophilic Glaciozyma antarctica PI12 that was exposed to heat stress. G. antarctica PI12 was grown at its optimal growth temperature of 12ºC and later exposed to heat stresses at 16ºC and 20ºC for 6 hours. Transcriptomes of those cells were extracted, sequenced and analyzed. Thirty-three molecular chaperone genes demonstrated differential expression of which 23 were up-regulated while 10 were down-regulated. Functions of up-regulated molecular chaperone genes were related to protein binding, response to a stimulus, chaperone binding, cellular response to stress, oxidation, and reduction, ATP binding, DNA-damage response and regulation for cellular protein metabolic process. On the other hand, functions of down-regulated molecular chaperone genes were related to chaperone-mediated protein complex assembly, transcription, cellular macromolecule metabolic process, regulation of cell growth and ribosome biogenesis. The findings provided information on how molecular chaperones work together in a complex network to protect the cells under heat stress. It also highlights the evolutionary conserved protective role of molecular chaperones in psychrophilic yeast, G. antarctica, and mesophilic yeast, Saccharomyces cerevisiae. |
format |
Article in Journal/Newspaper |
author |
Nur Athirah Yusof Clemente Michael Vui Ling Wong Abdul Munir Abdul Murad Farah Diba Abu Bakar Nor Muhammad Mahadi Ahmad Yamin Abdul Rahman Nursyafiqi Zainuddin Mohd Nazalan Mohd Najimudin |
author_facet |
Nur Athirah Yusof Clemente Michael Vui Ling Wong Abdul Munir Abdul Murad Farah Diba Abu Bakar Nor Muhammad Mahadi Ahmad Yamin Abdul Rahman Nursyafiqi Zainuddin Mohd Nazalan Mohd Najimudin |
author_sort |
Nur Athirah Yusof |
title |
Expression patterns of molecular chaperone genes in Antarctic psychrophilic yeast, Glaciozyma antarctica PI12 in response to heat stress |
title_short |
Expression patterns of molecular chaperone genes in Antarctic psychrophilic yeast, Glaciozyma antarctica PI12 in response to heat stress |
title_full |
Expression patterns of molecular chaperone genes in Antarctic psychrophilic yeast, Glaciozyma antarctica PI12 in response to heat stress |
title_fullStr |
Expression patterns of molecular chaperone genes in Antarctic psychrophilic yeast, Glaciozyma antarctica PI12 in response to heat stress |
title_full_unstemmed |
Expression patterns of molecular chaperone genes in Antarctic psychrophilic yeast, Glaciozyma antarctica PI12 in response to heat stress |
title_sort |
expression patterns of molecular chaperone genes in antarctic psychrophilic yeast, glaciozyma antarctica pi12 in response to heat stress |
publisher |
Polish Academy of Sciences |
publishDate |
2019 |
url |
https://doi.org/10.24425/ppr.2019.129674 https://doaj.org/article/05e8461d7f9e47038e410466140703d3 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica Polar Research |
genre_facet |
Antarc* Antarctic Antarctica Polar Research |
op_source |
Polish Polar Research, Vol vol. 40, Iss No 3, Pp 273-292 (2019) |
op_relation |
https://journals.pan.pl/Content/113185/PDF/PPR%203-19%205-N.Yusof.pdf https://doaj.org/toc/0138-0338 https://doaj.org/toc/2081-8262 0138-0338 2081-8262 https://doi.org/10.24425/ppr.2019.129674 https://doaj.org/article/05e8461d7f9e47038e410466140703d3 |
op_doi |
https://doi.org/10.24425/ppr.2019.129674 |
_version_ |
1766246798139064320 |