Protective immunity induced in mice by F8.1 and F8.2 antigens purified from Schistosoma mansoni eggs
Schistosoma mansoni soluble egg antigens (SEA) were fractionated by isoelectric focusing, resulting in 20 components, characterized by pH, absorbance and protein concentration. The higher absorbance fractions were submitted to electrophoresis, and fraction 8 (F8) presented a specific pattern of band...
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Instituto Oswaldo Cruz, Ministério da Saúde
1998
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ftdoajarticles:oai:doaj.org/article:0594dc7d72f749c28b4fc4e41f803d38 2023-05-15T15:07:04+02:00 Protective immunity induced in mice by F8.1 and F8.2 antigens purified from Schistosoma mansoni eggs Claudia Campra Ferreira Marcelo Matos Santoro Alfredo Miranda Goes 1998-01-01T00:00:00Z https://doi.org/10.1590/S0074-02761998000700031 https://doaj.org/article/0594dc7d72f749c28b4fc4e41f803d38 EN eng Instituto Oswaldo Cruz, Ministério da Saúde http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000700031 https://doaj.org/toc/0074-0276 https://doaj.org/toc/1678-8060 doi:10.1590/S0074-02761998000700031 0074-0276 1678-8060 https://doaj.org/article/0594dc7d72f749c28b4fc4e41f803d38 Memórias do Instituto Oswaldo Cruz., Vol 93, Pp 185-189 (1998) Schistosoma mansoni egg antigen purification immunity vaccine Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 article 1998 ftdoajarticles https://doi.org/10.1590/S0074-02761998000700031 2023-01-08T01:37:53Z Schistosoma mansoni soluble egg antigens (SEA) were fractionated by isoelectric focusing, resulting in 20 components, characterized by pH, absorbance and protein concentration. The higher absorbance fractions were submitted to electrophoresis, and fraction 8 (F8) presented a specific pattern of bands on its isoelectric point. Protein 3 was observed only on F8, and so, it was utilized to rabbit immunization, in order to evaluate its capacity of inducing protective immunity. IgG antibodies from rabbit anti-F8 serum were coupled to Sepharose, and used to obtain the specific antigen by affinity chromatography. This antigen, submitted to electrophoresis, presented two proteic bands (F8.1 and F8.2), which were transferred to nitrocellulose membrane (PVDF) and sequenciated. The homology of F8.2 to known proteins was determined using the Basic Local Alignment Search Tool program (BLASTp). Significant homologies were obtained for the rabbit cytosolic Ca2+ uptake inhibitor, and for the bird a1-proteinase inhibitor. Immunization of mice with F8.1 and F8.2, in the presence of Corynebacterium parvum and Al(OH)3 as adjuvant, induced a significant protection degree against challenge infection, as observed by the decrease on worm burden recovered from portal system. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Memórias do Instituto Oswaldo Cruz 93 suppl 1 185 189 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Schistosoma mansoni egg antigen purification immunity vaccine Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 |
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Schistosoma mansoni egg antigen purification immunity vaccine Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 Claudia Campra Ferreira Marcelo Matos Santoro Alfredo Miranda Goes Protective immunity induced in mice by F8.1 and F8.2 antigens purified from Schistosoma mansoni eggs |
topic_facet |
Schistosoma mansoni egg antigen purification immunity vaccine Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 |
description |
Schistosoma mansoni soluble egg antigens (SEA) were fractionated by isoelectric focusing, resulting in 20 components, characterized by pH, absorbance and protein concentration. The higher absorbance fractions were submitted to electrophoresis, and fraction 8 (F8) presented a specific pattern of bands on its isoelectric point. Protein 3 was observed only on F8, and so, it was utilized to rabbit immunization, in order to evaluate its capacity of inducing protective immunity. IgG antibodies from rabbit anti-F8 serum were coupled to Sepharose, and used to obtain the specific antigen by affinity chromatography. This antigen, submitted to electrophoresis, presented two proteic bands (F8.1 and F8.2), which were transferred to nitrocellulose membrane (PVDF) and sequenciated. The homology of F8.2 to known proteins was determined using the Basic Local Alignment Search Tool program (BLASTp). Significant homologies were obtained for the rabbit cytosolic Ca2+ uptake inhibitor, and for the bird a1-proteinase inhibitor. Immunization of mice with F8.1 and F8.2, in the presence of Corynebacterium parvum and Al(OH)3 as adjuvant, induced a significant protection degree against challenge infection, as observed by the decrease on worm burden recovered from portal system. |
format |
Article in Journal/Newspaper |
author |
Claudia Campra Ferreira Marcelo Matos Santoro Alfredo Miranda Goes |
author_facet |
Claudia Campra Ferreira Marcelo Matos Santoro Alfredo Miranda Goes |
author_sort |
Claudia Campra Ferreira |
title |
Protective immunity induced in mice by F8.1 and F8.2 antigens purified from Schistosoma mansoni eggs |
title_short |
Protective immunity induced in mice by F8.1 and F8.2 antigens purified from Schistosoma mansoni eggs |
title_full |
Protective immunity induced in mice by F8.1 and F8.2 antigens purified from Schistosoma mansoni eggs |
title_fullStr |
Protective immunity induced in mice by F8.1 and F8.2 antigens purified from Schistosoma mansoni eggs |
title_full_unstemmed |
Protective immunity induced in mice by F8.1 and F8.2 antigens purified from Schistosoma mansoni eggs |
title_sort |
protective immunity induced in mice by f8.1 and f8.2 antigens purified from schistosoma mansoni eggs |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
1998 |
url |
https://doi.org/10.1590/S0074-02761998000700031 https://doaj.org/article/0594dc7d72f749c28b4fc4e41f803d38 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Memórias do Instituto Oswaldo Cruz., Vol 93, Pp 185-189 (1998) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000700031 https://doaj.org/toc/0074-0276 https://doaj.org/toc/1678-8060 doi:10.1590/S0074-02761998000700031 0074-0276 1678-8060 https://doaj.org/article/0594dc7d72f749c28b4fc4e41f803d38 |
op_doi |
https://doi.org/10.1590/S0074-02761998000700031 |
container_title |
Memórias do Instituto Oswaldo Cruz |
container_volume |
93 |
container_issue |
suppl 1 |
container_start_page |
185 |
op_container_end_page |
189 |
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1766338640567336960 |