Evaluation of Leishmania (Leishmania) chagasi strains isolated from dogs originating from two visceral leishmaniasis-endemic areas in Brazil using multilocus enzyme electrophoresis
INTRODUCTION: Domestic dogs are the most important reservoir in the peridomestic transmission cycle of Leishmania (Leishmania) chagasi. The genetic variability of subpopulations of this parasite circulating in dogs has not been thoroughly analyzed in Brazil, even though this knowledge has important...
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ftdoajarticles:oai:doaj.org/article:0587c34eb32a4f3195b9dd2e7a745d36 2023-05-15T15:07:22+02:00 Evaluation of Leishmania (Leishmania) chagasi strains isolated from dogs originating from two visceral leishmaniasis-endemic areas in Brazil using multilocus enzyme electrophoresis Carlos Eduardo Ribeiro Coutinho Dilvani Oliveira Santos Cibele Baptista Fabiano Borges Figueiredo Maria de Fatima Madeira 2011-10-01T00:00:00Z https://doi.org/10.1590/s0037-86822011005000049 https://doaj.org/article/0587c34eb32a4f3195b9dd2e7a745d36 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000500008&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/s0037-86822011005000049 https://doaj.org/article/0587c34eb32a4f3195b9dd2e7a745d36 Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 5, Pp 572-575 (2011) Cão Leishmania chagasi Variabilidade genética MLEE Arctic medicine. Tropical medicine RC955-962 article 2011 ftdoajarticles https://doi.org/10.1590/s0037-86822011005000049 2022-12-31T02:54:56Z INTRODUCTION: Domestic dogs are the most important reservoir in the peridomestic transmission cycle of Leishmania (Leishmania) chagasi. The genetic variability of subpopulations of this parasite circulating in dogs has not been thoroughly analyzed in Brazil, even though this knowledge has important implications in the clinical-epidemiological context. METHODS: The objective of this study was to evaluate and compare the phenotypic variability of 153 L. chagasi strains isolated from dogs originating from the municipalities of Rio de Janeiro (n = 57) and Belo Horizonte (n = 96), where the disease is endemic. Strains isolated only from intact skin were selected and analyzed by multilocus enzyme electrophoresis using nine enzyme systems (6PG, GPI, NH1 and NH2, G6P, PGM, MDH, ME, and IDHNADP). RESULTS: The electrophoretic profile was identical for all isolates analyzed and was the same as that of the L. chagasi reference strain (MHOM/BR/74/PP75). Phenetic analysis showed a similarity index of one for all strains, with the isolates sharing 100% of the characteristics analyzed. CONCLUSIONS: The results demonstrate that the L. chagasi populations circulating in dogs from Rio de Janeiro and Belo Horizonte belong to a single zymodeme. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 44 5 572 575 |
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Cão Leishmania chagasi Variabilidade genética MLEE Arctic medicine. Tropical medicine RC955-962 |
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Cão Leishmania chagasi Variabilidade genética MLEE Arctic medicine. Tropical medicine RC955-962 Carlos Eduardo Ribeiro Coutinho Dilvani Oliveira Santos Cibele Baptista Fabiano Borges Figueiredo Maria de Fatima Madeira Evaluation of Leishmania (Leishmania) chagasi strains isolated from dogs originating from two visceral leishmaniasis-endemic areas in Brazil using multilocus enzyme electrophoresis |
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Cão Leishmania chagasi Variabilidade genética MLEE Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: Domestic dogs are the most important reservoir in the peridomestic transmission cycle of Leishmania (Leishmania) chagasi. The genetic variability of subpopulations of this parasite circulating in dogs has not been thoroughly analyzed in Brazil, even though this knowledge has important implications in the clinical-epidemiological context. METHODS: The objective of this study was to evaluate and compare the phenotypic variability of 153 L. chagasi strains isolated from dogs originating from the municipalities of Rio de Janeiro (n = 57) and Belo Horizonte (n = 96), where the disease is endemic. Strains isolated only from intact skin were selected and analyzed by multilocus enzyme electrophoresis using nine enzyme systems (6PG, GPI, NH1 and NH2, G6P, PGM, MDH, ME, and IDHNADP). RESULTS: The electrophoretic profile was identical for all isolates analyzed and was the same as that of the L. chagasi reference strain (MHOM/BR/74/PP75). Phenetic analysis showed a similarity index of one for all strains, with the isolates sharing 100% of the characteristics analyzed. CONCLUSIONS: The results demonstrate that the L. chagasi populations circulating in dogs from Rio de Janeiro and Belo Horizonte belong to a single zymodeme. |
format |
Article in Journal/Newspaper |
author |
Carlos Eduardo Ribeiro Coutinho Dilvani Oliveira Santos Cibele Baptista Fabiano Borges Figueiredo Maria de Fatima Madeira |
author_facet |
Carlos Eduardo Ribeiro Coutinho Dilvani Oliveira Santos Cibele Baptista Fabiano Borges Figueiredo Maria de Fatima Madeira |
author_sort |
Carlos Eduardo Ribeiro Coutinho |
title |
Evaluation of Leishmania (Leishmania) chagasi strains isolated from dogs originating from two visceral leishmaniasis-endemic areas in Brazil using multilocus enzyme electrophoresis |
title_short |
Evaluation of Leishmania (Leishmania) chagasi strains isolated from dogs originating from two visceral leishmaniasis-endemic areas in Brazil using multilocus enzyme electrophoresis |
title_full |
Evaluation of Leishmania (Leishmania) chagasi strains isolated from dogs originating from two visceral leishmaniasis-endemic areas in Brazil using multilocus enzyme electrophoresis |
title_fullStr |
Evaluation of Leishmania (Leishmania) chagasi strains isolated from dogs originating from two visceral leishmaniasis-endemic areas in Brazil using multilocus enzyme electrophoresis |
title_full_unstemmed |
Evaluation of Leishmania (Leishmania) chagasi strains isolated from dogs originating from two visceral leishmaniasis-endemic areas in Brazil using multilocus enzyme electrophoresis |
title_sort |
evaluation of leishmania (leishmania) chagasi strains isolated from dogs originating from two visceral leishmaniasis-endemic areas in brazil using multilocus enzyme electrophoresis |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2011 |
url |
https://doi.org/10.1590/s0037-86822011005000049 https://doaj.org/article/0587c34eb32a4f3195b9dd2e7a745d36 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 5, Pp 572-575 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000500008&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/s0037-86822011005000049 https://doaj.org/article/0587c34eb32a4f3195b9dd2e7a745d36 |
op_doi |
https://doi.org/10.1590/s0037-86822011005000049 |
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