Susceptibility of Biomphalaria tenagophila and Biomphalaria straminea to Schistosoma mansoni infection detected by low stringency polymerase chain reaction

• Main Authors: JANNOTTI-PASSOS Liana Konovaloff, SOUZA Cecilia Pereira de
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 2000
• Subjects: Schistosoma mansoni; Biomphalaria tenagophila; Biomphalaria straminea; Susceptibility; LS-PCR technique; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic
• Online Access: https://doaj.org/article/0577b40b8f1e4d5c946b6294d4da57af

In order to determine Schistosoma mansoni infection rates in Biomphalaria tenagophila and B. straminea, low stringency polymerase chain reaction (LS-PCR) technique was used as a complementary method to light exposure technique. LS-PCR has already been standardized in our laboratory to detect the trematode DNA in B. glabrata. Higher S. mansoni infection rates were detected using conventional method and LS-PCR. The parasite DNA profile was detected in both species after 7-day exposure to miracidia, using LS-PCR. This technique enables early detection of schistosomiasis transmission focuses, in endemic areas, before the beginning of cercariae shedding.