Susceptibility of Biomphalaria tenagophila and Biomphalaria straminea to Schistosoma mansoni infection detected by low stringency polymerase chain reaction

In order to determine Schistosoma mansoni infection rates in Biomphalaria tenagophila and B. straminea, low stringency polymerase chain reaction (LS-PCR) technique was used as a complementary method to light exposure technique. LS-PCR has already been standardized in our laboratory to detect the tre...

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Bibliographic Details
Main Authors: JANNOTTI-PASSOS Liana Konovaloff, SOUZA Cecilia Pereira de
Format: Article in Journal/Newspaper
Language:English
Published: Universidade de São Paulo (USP) 2000
Subjects:
Schistosoma mansoni
Biomphalaria tenagophila
Biomphalaria straminea
Susceptibility
LS-PCR technique
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doaj.org/article/0577b40b8f1e4d5c946b6294d4da57af
Description
Summary:In order to determine Schistosoma mansoni infection rates in Biomphalaria tenagophila and B. straminea, low stringency polymerase chain reaction (LS-PCR) technique was used as a complementary method to light exposure technique. LS-PCR has already been standardized in our laboratory to detect the trematode DNA in B. glabrata. Higher S. mansoni infection rates were detected using conventional method and LS-PCR. The parasite DNA profile was detected in both species after 7-day exposure to miracidia, using LS-PCR. This technique enables early detection of schistosomiasis transmission focuses, in endemic areas, before the beginning of cercariae shedding.

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