DNA Methylation Changes Induced by Cold in Psychrophilic and Psychrotolerant Naganishia Yeast Species
The involvement of DNA methylation in the response to cold stress of two different yeast species ( Naganishia antarctica , psychrophilic, and Naganishia albida , psychrotolerant), exhibiting different temperature aptitudes, has been studied. Consecutive incubations at respective optimum temperatures...
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ftdoajarticles:oai:doaj.org/article:0568139fedaa40fe97fab5f9c8c5f0c2 2023-05-15T13:55:26+02:00 DNA Methylation Changes Induced by Cold in Psychrophilic and Psychrotolerant Naganishia Yeast Species Benedetta Turchetti Gianpiero Marconi Ciro Sannino Pietro Buzzini Emidio Albertini 2020-02-01T00:00:00Z https://doi.org/10.3390/microorganisms8020296 https://doaj.org/article/0568139fedaa40fe97fab5f9c8c5f0c2 EN eng MDPI AG https://www.mdpi.com/2076-2607/8/2/296 https://doaj.org/toc/2076-2607 2076-2607 doi:10.3390/microorganisms8020296 https://doaj.org/article/0568139fedaa40fe97fab5f9c8c5f0c2 Microorganisms, Vol 8, Iss 2, p 296 (2020) cold-adapted yeasts cold stress response naganishia albida naganishia antarctica Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.3390/microorganisms8020296 2022-12-31T00:14:45Z The involvement of DNA methylation in the response to cold stress of two different yeast species ( Naganishia antarctica , psychrophilic, and Naganishia albida , psychrotolerant), exhibiting different temperature aptitudes, has been studied. Consecutive incubations at respective optimum temperatures, at 4 °C (cold stress) and at optimum temperatures again, were performed. After Methylation Sensitive Amplified Polymorphism (MSAP) fingerprints a total of 550 and 423 clear and reproducible fragments were amplified from N. antarctica and N. albida strains, respectively. The two Naganishia strains showed a different response in terms of level of DNA methylation during cold stress and recovery from cold stress. The percentage of total methylated fragments in psychrophilic N. antarctica did not show any significant change. On the contrary, the methylation of psychrotolerant N. albida exhibited a nonsignificant increase during the incubation at 4 °C and continued during the recovery step, showing a significant difference if compared with control condition, resembling an uncontrolled response to cold stress. A total of 12 polymorphic fragments were selected, cloned, and sequenced. Four fragments were associated to genes encoding for elongation factor G and for chitin synthase export chaperon. To the best of our knowledge, this is the first study on DNA methylation in the response to cold stress carried out by comparing a psychrophilic and a psychrotolerant yeast species. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Microorganisms 8 2 296 |
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language |
English |
topic |
cold-adapted yeasts cold stress response naganishia albida naganishia antarctica Biology (General) QH301-705.5 |
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cold-adapted yeasts cold stress response naganishia albida naganishia antarctica Biology (General) QH301-705.5 Benedetta Turchetti Gianpiero Marconi Ciro Sannino Pietro Buzzini Emidio Albertini DNA Methylation Changes Induced by Cold in Psychrophilic and Psychrotolerant Naganishia Yeast Species |
topic_facet |
cold-adapted yeasts cold stress response naganishia albida naganishia antarctica Biology (General) QH301-705.5 |
description |
The involvement of DNA methylation in the response to cold stress of two different yeast species ( Naganishia antarctica , psychrophilic, and Naganishia albida , psychrotolerant), exhibiting different temperature aptitudes, has been studied. Consecutive incubations at respective optimum temperatures, at 4 °C (cold stress) and at optimum temperatures again, were performed. After Methylation Sensitive Amplified Polymorphism (MSAP) fingerprints a total of 550 and 423 clear and reproducible fragments were amplified from N. antarctica and N. albida strains, respectively. The two Naganishia strains showed a different response in terms of level of DNA methylation during cold stress and recovery from cold stress. The percentage of total methylated fragments in psychrophilic N. antarctica did not show any significant change. On the contrary, the methylation of psychrotolerant N. albida exhibited a nonsignificant increase during the incubation at 4 °C and continued during the recovery step, showing a significant difference if compared with control condition, resembling an uncontrolled response to cold stress. A total of 12 polymorphic fragments were selected, cloned, and sequenced. Four fragments were associated to genes encoding for elongation factor G and for chitin synthase export chaperon. To the best of our knowledge, this is the first study on DNA methylation in the response to cold stress carried out by comparing a psychrophilic and a psychrotolerant yeast species. |
format |
Article in Journal/Newspaper |
author |
Benedetta Turchetti Gianpiero Marconi Ciro Sannino Pietro Buzzini Emidio Albertini |
author_facet |
Benedetta Turchetti Gianpiero Marconi Ciro Sannino Pietro Buzzini Emidio Albertini |
author_sort |
Benedetta Turchetti |
title |
DNA Methylation Changes Induced by Cold in Psychrophilic and Psychrotolerant Naganishia Yeast Species |
title_short |
DNA Methylation Changes Induced by Cold in Psychrophilic and Psychrotolerant Naganishia Yeast Species |
title_full |
DNA Methylation Changes Induced by Cold in Psychrophilic and Psychrotolerant Naganishia Yeast Species |
title_fullStr |
DNA Methylation Changes Induced by Cold in Psychrophilic and Psychrotolerant Naganishia Yeast Species |
title_full_unstemmed |
DNA Methylation Changes Induced by Cold in Psychrophilic and Psychrotolerant Naganishia Yeast Species |
title_sort |
dna methylation changes induced by cold in psychrophilic and psychrotolerant naganishia yeast species |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/microorganisms8020296 https://doaj.org/article/0568139fedaa40fe97fab5f9c8c5f0c2 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Microorganisms, Vol 8, Iss 2, p 296 (2020) |
op_relation |
https://www.mdpi.com/2076-2607/8/2/296 https://doaj.org/toc/2076-2607 2076-2607 doi:10.3390/microorganisms8020296 https://doaj.org/article/0568139fedaa40fe97fab5f9c8c5f0c2 |
op_doi |
https://doi.org/10.3390/microorganisms8020296 |
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Microorganisms |
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8 |
container_issue |
2 |
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296 |
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1766262055126433792 |