Putative new heat-stable cytotoxic and enterotoxic factors in culture supernatant of Escherichia coli isolated from drinking water
Enteric infections caused by the ingestion of contaminated water, especially by Escherichia coli, are important to define the virulence properties of these bacteria. Due to frequent infantile diarrhea in the city of Ouro Preto, Minas Gerais state, Brazil, the phenotypic and genotypic diarrheagenic p...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:055859a7e4b24fe0b2c7e473ca18a00f 2023-05-15T15:07:45+02:00 Putative new heat-stable cytotoxic and enterotoxic factors in culture supernatant of Escherichia coli isolated from drinking water DA Ribeiro FS Niemann MSV Gatti MCS Lanna T Tsuji T Yano 2011-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992011000100014 https://doaj.org/article/055859a7e4b24fe0b2c7e473ca18a00f EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100014 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992011000100014 1678-9199 https://doaj.org/article/055859a7e4b24fe0b2c7e473ca18a00f Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 17, Iss 1, Pp 103-107 (2011) Escherichia coli drinking water cytotoxin enterotoxin Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2011 ftdoajarticles https://doi.org/10.1590/S1678-91992011000100014 2022-12-31T16:03:31Z Enteric infections caused by the ingestion of contaminated water, especially by Escherichia coli, are important to define the virulence properties of these bacteria. Due to frequent infantile diarrhea in the city of Ouro Preto, Minas Gerais state, Brazil, the phenotypic and genotypic diarrheagenic properties of E. coli isolated from drinking water were studied. The culture supernatants of 39 (40%) among a total of 97 E. coli isolates from drinking water were positive by suckling mouse assay and induced cytotoxic effects on Vero cells. The enterotoxic and cytotoxic activities were present in the fraction with less than 10 kDa and were not lost when heated up to 60°C and 100°C for 30 minutes. PCR assays showed that among these 39 Vero cytotoxigenic E. coli, four (10.2%) were positive for ST II (estB) and two (5%) positive for αHly (hlyA). Gene amplification of SLT (stx 1, stx 2), ST I (estA), LT (eltI, eltII), EAST1 (astA), EHly (enhly) and plasmid-encoded enterotoxin (pet) were not observed. This heat-stable cytotoxic enterotoxin of E. coli is probably a new putative diarrheagenic virulence factor, as a toxin presenting these characteristics has not yet been described. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 17 1 103 107 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Escherichia coli drinking water cytotoxin enterotoxin Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Escherichia coli drinking water cytotoxin enterotoxin Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 DA Ribeiro FS Niemann MSV Gatti MCS Lanna T Tsuji T Yano Putative new heat-stable cytotoxic and enterotoxic factors in culture supernatant of Escherichia coli isolated from drinking water |
topic_facet |
Escherichia coli drinking water cytotoxin enterotoxin Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Enteric infections caused by the ingestion of contaminated water, especially by Escherichia coli, are important to define the virulence properties of these bacteria. Due to frequent infantile diarrhea in the city of Ouro Preto, Minas Gerais state, Brazil, the phenotypic and genotypic diarrheagenic properties of E. coli isolated from drinking water were studied. The culture supernatants of 39 (40%) among a total of 97 E. coli isolates from drinking water were positive by suckling mouse assay and induced cytotoxic effects on Vero cells. The enterotoxic and cytotoxic activities were present in the fraction with less than 10 kDa and were not lost when heated up to 60°C and 100°C for 30 minutes. PCR assays showed that among these 39 Vero cytotoxigenic E. coli, four (10.2%) were positive for ST II (estB) and two (5%) positive for αHly (hlyA). Gene amplification of SLT (stx 1, stx 2), ST I (estA), LT (eltI, eltII), EAST1 (astA), EHly (enhly) and plasmid-encoded enterotoxin (pet) were not observed. This heat-stable cytotoxic enterotoxin of E. coli is probably a new putative diarrheagenic virulence factor, as a toxin presenting these characteristics has not yet been described. |
format |
Article in Journal/Newspaper |
author |
DA Ribeiro FS Niemann MSV Gatti MCS Lanna T Tsuji T Yano |
author_facet |
DA Ribeiro FS Niemann MSV Gatti MCS Lanna T Tsuji T Yano |
author_sort |
DA Ribeiro |
title |
Putative new heat-stable cytotoxic and enterotoxic factors in culture supernatant of Escherichia coli isolated from drinking water |
title_short |
Putative new heat-stable cytotoxic and enterotoxic factors in culture supernatant of Escherichia coli isolated from drinking water |
title_full |
Putative new heat-stable cytotoxic and enterotoxic factors in culture supernatant of Escherichia coli isolated from drinking water |
title_fullStr |
Putative new heat-stable cytotoxic and enterotoxic factors in culture supernatant of Escherichia coli isolated from drinking water |
title_full_unstemmed |
Putative new heat-stable cytotoxic and enterotoxic factors in culture supernatant of Escherichia coli isolated from drinking water |
title_sort |
putative new heat-stable cytotoxic and enterotoxic factors in culture supernatant of escherichia coli isolated from drinking water |
publisher |
SciELO |
publishDate |
2011 |
url |
https://doi.org/10.1590/S1678-91992011000100014 https://doaj.org/article/055859a7e4b24fe0b2c7e473ca18a00f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 17, Iss 1, Pp 103-107 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100014 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992011000100014 1678-9199 https://doaj.org/article/055859a7e4b24fe0b2c7e473ca18a00f |
op_doi |
https://doi.org/10.1590/S1678-91992011000100014 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
17 |
container_issue |
1 |
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103 |
op_container_end_page |
107 |
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1766339188190347264 |