Unveiling biosynthetic potential of an Arctic marine-derived strain Aspergillus sydowii MNP-2
Abstract Background A growing number of studies have demonstrated that the polar regions have the potential to be a significant repository of microbial resources and a potential source of active ingredients. Genome mining strategy plays a key role in the discovery of bioactive secondary metabolites...
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ftdoajarticles:oai:doaj.org/article:0540d379eef24744b0269970ad493528 2024-09-09T19:23:22+00:00 Unveiling biosynthetic potential of an Arctic marine-derived strain Aspergillus sydowii MNP-2 Zhiyang Fu Xiangzhou Gong Zhe Hu Bin Wei Huawei Zhang 2024-06-01T00:00:00Z https://doi.org/10.1186/s12864-024-10501-0 https://doaj.org/article/0540d379eef24744b0269970ad493528 EN eng BMC https://doi.org/10.1186/s12864-024-10501-0 https://doaj.org/toc/1471-2164 doi:10.1186/s12864-024-10501-0 1471-2164 https://doaj.org/article/0540d379eef24744b0269970ad493528 BMC Genomics, Vol 25, Iss 1, Pp 1-14 (2024) Polar microorganisms Aspergillus Sydowii Whole-genome sequence Biosynthetic gene cluster antiSMASH Molecular networking Biotechnology TP248.13-248.65 Genetics QH426-470 article 2024 ftdoajarticles https://doi.org/10.1186/s12864-024-10501-0 2024-08-05T17:49:08Z Abstract Background A growing number of studies have demonstrated that the polar regions have the potential to be a significant repository of microbial resources and a potential source of active ingredients. Genome mining strategy plays a key role in the discovery of bioactive secondary metabolites (SMs) from microorganisms. This work highlighted deciphering the biosynthetic potential of an Arctic marine-derived strain Aspergillus sydowii MNP-2 by a combination of whole genome analysis and antiSMASH as well as feature-based molecular networking (MN) in the Global Natural Products Social Molecular Networking (GNPS). Results In this study, a high-quality whole genome sequence of an Arctic marine strain MNP-2, with a size of 34.9 Mb was successfully obtained. Its total number of genes predicted by BRAKER software was 13,218, and that of non-coding RNAs (rRNA, sRNA, snRNA, and tRNA) predicted by using INFERNAL software was 204. AntiSMASH results indicated that strain MNP-2 harbors 56 biosynthetic gene clusters (BGCs), including 18 NRPS/NRPS-like gene clusters, 10 PKS/PKS-like gene clusters, 8 terpene synthse gene clusters, 5 indole synthase gene clusters, 10 hybrid gene clusters, and 5 fungal-RiPP gene clusters. Metabolic analyses of strain MNP-2 grown on various media using GNPS networking revealed its great potential for the biosynthesis of bioactive SMs containing a variety of heterocyclic and bridge-ring structures. For example, compound G-8 exhibited a potent anti-HIV effect with an IC50 value of 7.2 nM and an EC50 value of 0.9 nM. Compound G-6 had excellent in vitro cytotoxicities against the K562, MCF-7, Hela, DU145, U1975, SGC-7901, A549, MOLT-4, and HL60 cell lines, with IC50 values ranging from 0.10 to 3.3 µM, and showed significant anti-viral (H1N1 and H3N2) activities with IC50 values of 15.9 and 30.0 µM, respectively. Conclusions These findings definitely improve our knowledge about the molecular biology of genus A. sydowii and would effectively unveil the biosynthetic potential of strain MNP-2 using ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic BMC Genomics 25 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Polar microorganisms Aspergillus Sydowii Whole-genome sequence Biosynthetic gene cluster antiSMASH Molecular networking Biotechnology TP248.13-248.65 Genetics QH426-470 |
spellingShingle |
Polar microorganisms Aspergillus Sydowii Whole-genome sequence Biosynthetic gene cluster antiSMASH Molecular networking Biotechnology TP248.13-248.65 Genetics QH426-470 Zhiyang Fu Xiangzhou Gong Zhe Hu Bin Wei Huawei Zhang Unveiling biosynthetic potential of an Arctic marine-derived strain Aspergillus sydowii MNP-2 |
topic_facet |
Polar microorganisms Aspergillus Sydowii Whole-genome sequence Biosynthetic gene cluster antiSMASH Molecular networking Biotechnology TP248.13-248.65 Genetics QH426-470 |
description |
Abstract Background A growing number of studies have demonstrated that the polar regions have the potential to be a significant repository of microbial resources and a potential source of active ingredients. Genome mining strategy plays a key role in the discovery of bioactive secondary metabolites (SMs) from microorganisms. This work highlighted deciphering the biosynthetic potential of an Arctic marine-derived strain Aspergillus sydowii MNP-2 by a combination of whole genome analysis and antiSMASH as well as feature-based molecular networking (MN) in the Global Natural Products Social Molecular Networking (GNPS). Results In this study, a high-quality whole genome sequence of an Arctic marine strain MNP-2, with a size of 34.9 Mb was successfully obtained. Its total number of genes predicted by BRAKER software was 13,218, and that of non-coding RNAs (rRNA, sRNA, snRNA, and tRNA) predicted by using INFERNAL software was 204. AntiSMASH results indicated that strain MNP-2 harbors 56 biosynthetic gene clusters (BGCs), including 18 NRPS/NRPS-like gene clusters, 10 PKS/PKS-like gene clusters, 8 terpene synthse gene clusters, 5 indole synthase gene clusters, 10 hybrid gene clusters, and 5 fungal-RiPP gene clusters. Metabolic analyses of strain MNP-2 grown on various media using GNPS networking revealed its great potential for the biosynthesis of bioactive SMs containing a variety of heterocyclic and bridge-ring structures. For example, compound G-8 exhibited a potent anti-HIV effect with an IC50 value of 7.2 nM and an EC50 value of 0.9 nM. Compound G-6 had excellent in vitro cytotoxicities against the K562, MCF-7, Hela, DU145, U1975, SGC-7901, A549, MOLT-4, and HL60 cell lines, with IC50 values ranging from 0.10 to 3.3 µM, and showed significant anti-viral (H1N1 and H3N2) activities with IC50 values of 15.9 and 30.0 µM, respectively. Conclusions These findings definitely improve our knowledge about the molecular biology of genus A. sydowii and would effectively unveil the biosynthetic potential of strain MNP-2 using ... |
format |
Article in Journal/Newspaper |
author |
Zhiyang Fu Xiangzhou Gong Zhe Hu Bin Wei Huawei Zhang |
author_facet |
Zhiyang Fu Xiangzhou Gong Zhe Hu Bin Wei Huawei Zhang |
author_sort |
Zhiyang Fu |
title |
Unveiling biosynthetic potential of an Arctic marine-derived strain Aspergillus sydowii MNP-2 |
title_short |
Unveiling biosynthetic potential of an Arctic marine-derived strain Aspergillus sydowii MNP-2 |
title_full |
Unveiling biosynthetic potential of an Arctic marine-derived strain Aspergillus sydowii MNP-2 |
title_fullStr |
Unveiling biosynthetic potential of an Arctic marine-derived strain Aspergillus sydowii MNP-2 |
title_full_unstemmed |
Unveiling biosynthetic potential of an Arctic marine-derived strain Aspergillus sydowii MNP-2 |
title_sort |
unveiling biosynthetic potential of an arctic marine-derived strain aspergillus sydowii mnp-2 |
publisher |
BMC |
publishDate |
2024 |
url |
https://doi.org/10.1186/s12864-024-10501-0 https://doaj.org/article/0540d379eef24744b0269970ad493528 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
BMC Genomics, Vol 25, Iss 1, Pp 1-14 (2024) |
op_relation |
https://doi.org/10.1186/s12864-024-10501-0 https://doaj.org/toc/1471-2164 doi:10.1186/s12864-024-10501-0 1471-2164 https://doaj.org/article/0540d379eef24744b0269970ad493528 |
op_doi |
https://doi.org/10.1186/s12864-024-10501-0 |
container_title |
BMC Genomics |
container_volume |
25 |
container_issue |
1 |
_version_ |
1809763790501183488 |