Cytoskeleton, endoplasmic reticulum and nucleus alterations in CHO-K1 cell line after Crotalus durissus terrificus (South American rattlesnake) venom treatment
Snake venoms are toxic to a variety of cell types. However, the intracellular damages and the cell death fate induced by venom are unclear. In the present work, the action of the South American rattlesnake Crotalus durissus terrificus venom on CHO-K1 cell line was analyzed. The cells CHO-K1 were inc...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:04ea422e293a42089b66b734654d12aa 2023-05-15T15:03:05+02:00 Cytoskeleton, endoplasmic reticulum and nucleus alterations in CHO-K1 cell line after Crotalus durissus terrificus (South American rattlesnake) venom treatment B. P. Tamieti R. A. Damatta J. C. Cogo N. S. Da Silva J. Mittmann C. Pacheco-Soares 2007-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992007000100004 https://doaj.org/article/04ea422e293a42089b66b734654d12aa EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100004 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992007000100004 1678-9199 https://doaj.org/article/04ea422e293a42089b66b734654d12aa Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 13, Iss 1, Pp 56-68 (2007) cell culture actin filaments nucleus endoplasmic reticulum Crotalus rattlesnake venom apoptosis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2007 ftdoajarticles https://doi.org/10.1590/S1678-91992007000100004 2022-12-31T11:22:26Z Snake venoms are toxic to a variety of cell types. However, the intracellular damages and the cell death fate induced by venom are unclear. In the present work, the action of the South American rattlesnake Crotalus durissus terrificus venom on CHO-K1 cell line was analyzed. The cells CHO-K1 were incubated with C. d. terrificus venom (10, 50 and 100g/ml) for 1 and 24 hours, and structural alterations of actin filaments, endoplasmic reticulum and nucleus were assessed using specific fluorescent probes and agarose gel electrophoresis for DNA fragmentation. Significant structural changes were observed in all analyzed structures. DNA fragmentation was detected suggesting that, at the concentrations used, the venom induced apoptosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 13 1 56 68 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
cell culture actin filaments nucleus endoplasmic reticulum Crotalus rattlesnake venom apoptosis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
cell culture actin filaments nucleus endoplasmic reticulum Crotalus rattlesnake venom apoptosis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 B. P. Tamieti R. A. Damatta J. C. Cogo N. S. Da Silva J. Mittmann C. Pacheco-Soares Cytoskeleton, endoplasmic reticulum and nucleus alterations in CHO-K1 cell line after Crotalus durissus terrificus (South American rattlesnake) venom treatment |
topic_facet |
cell culture actin filaments nucleus endoplasmic reticulum Crotalus rattlesnake venom apoptosis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Snake venoms are toxic to a variety of cell types. However, the intracellular damages and the cell death fate induced by venom are unclear. In the present work, the action of the South American rattlesnake Crotalus durissus terrificus venom on CHO-K1 cell line was analyzed. The cells CHO-K1 were incubated with C. d. terrificus venom (10, 50 and 100g/ml) for 1 and 24 hours, and structural alterations of actin filaments, endoplasmic reticulum and nucleus were assessed using specific fluorescent probes and agarose gel electrophoresis for DNA fragmentation. Significant structural changes were observed in all analyzed structures. DNA fragmentation was detected suggesting that, at the concentrations used, the venom induced apoptosis. |
format |
Article in Journal/Newspaper |
author |
B. P. Tamieti R. A. Damatta J. C. Cogo N. S. Da Silva J. Mittmann C. Pacheco-Soares |
author_facet |
B. P. Tamieti R. A. Damatta J. C. Cogo N. S. Da Silva J. Mittmann C. Pacheco-Soares |
author_sort |
B. P. Tamieti |
title |
Cytoskeleton, endoplasmic reticulum and nucleus alterations in CHO-K1 cell line after Crotalus durissus terrificus (South American rattlesnake) venom treatment |
title_short |
Cytoskeleton, endoplasmic reticulum and nucleus alterations in CHO-K1 cell line after Crotalus durissus terrificus (South American rattlesnake) venom treatment |
title_full |
Cytoskeleton, endoplasmic reticulum and nucleus alterations in CHO-K1 cell line after Crotalus durissus terrificus (South American rattlesnake) venom treatment |
title_fullStr |
Cytoskeleton, endoplasmic reticulum and nucleus alterations in CHO-K1 cell line after Crotalus durissus terrificus (South American rattlesnake) venom treatment |
title_full_unstemmed |
Cytoskeleton, endoplasmic reticulum and nucleus alterations in CHO-K1 cell line after Crotalus durissus terrificus (South American rattlesnake) venom treatment |
title_sort |
cytoskeleton, endoplasmic reticulum and nucleus alterations in cho-k1 cell line after crotalus durissus terrificus (south american rattlesnake) venom treatment |
publisher |
SciELO |
publishDate |
2007 |
url |
https://doi.org/10.1590/S1678-91992007000100004 https://doaj.org/article/04ea422e293a42089b66b734654d12aa |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 13, Iss 1, Pp 56-68 (2007) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100004 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992007000100004 1678-9199 https://doaj.org/article/04ea422e293a42089b66b734654d12aa |
op_doi |
https://doi.org/10.1590/S1678-91992007000100004 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
13 |
container_issue |
1 |
container_start_page |
56 |
op_container_end_page |
68 |
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1766334986838867968 |