Oxidative stress induction by crude extract of Xylaria sp. triggers lethality in the larvae of Aedes aegypti (Diptera: Culicidae)
ABSTRACT Background: Aedes aegypti is currently controlled with synthetic larvicides; however, mosquitoes have become highly resistant to these larvicides and difficult to eradicate. Studies have shown that insecticides derived from fungal extracts have various mechanisms of action that reduce the r...
Published in: | Revista da Sociedade Brasileira de Medicina Tropical |
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Sociedade Brasileira de Medicina Tropical (SBMT)
2022
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Online Access: | https://doi.org/10.1590/0037-8682-0373-2021 https://doaj.org/article/04d91dd2867d4cf5b6675f3685b818e5 |
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ftdoajarticles:oai:doaj.org/article:04d91dd2867d4cf5b6675f3685b818e5 2023-05-15T15:11:06+02:00 Oxidative stress induction by crude extract of Xylaria sp. triggers lethality in the larvae of Aedes aegypti (Diptera: Culicidae) Maria Beatriz Silva Costa Rejane de Castro Simões Márcia de Jesus Amazonas da Silva André Correa de Oliveira Leonard Domingo Rosales Acho Emerson Silva Lima Wanderli Pedro Tadei Helder Lopes Teles Camila Martins de Oliveira 2022-04-01T00:00:00Z https://doi.org/10.1590/0037-8682-0373-2021 https://doaj.org/article/04d91dd2867d4cf5b6675f3685b818e5 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822022000100312&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0373-2021 https://doaj.org/article/04d91dd2867d4cf5b6675f3685b818e5 Revista da Sociedade Brasileira de Medicina Tropical, Vol 55 (2022) Cytotoxicity Carbonyl Dengue TBARS Endophytic fungus Arctic medicine. Tropical medicine RC955-962 article 2022 ftdoajarticles https://doi.org/10.1590/0037-8682-0373-2021 2022-12-31T02:28:59Z ABSTRACT Background: Aedes aegypti is currently controlled with synthetic larvicides; however, mosquitoes have become highly resistant to these larvicides and difficult to eradicate. Studies have shown that insecticides derived from fungal extracts have various mechanisms of action that reduce the risk of resistance in these mosquitoes. One possible mechanism is uncontrolled production of reactive oxygen species (ROS) in the larvae, which can cause changes at the cellular level. Thus, the crude extract of Xylaria sp. was evaluated to investigate the oxidative effect of this extract in A. aegypti larvae by quantifying the oxidative damage to proteins and lipids. Methods: The larvicidal potential of the crude extract of Xylaria sp. Was evaluated, and the extract was subsequently tested in human lung fibroblasts for cytotoxicity and ROS production. ROS level was quantified in the larvae that were killed following exposure to the extract in the larvicide test. Results: The crude extract of Xylaria sp. Caused cytotoxicity and induced ROS production in human lung fibroblasts and A. aegypti larvae, respectively. In the larvicide trial, the extract showed an LC50 of 264.456 ppm and an LC90 of 364.307 ppm, and was thus considered active. The extract showed greater oxidative damage to lipids and proteins, with LC90 values of 24.7 µmol MDA/L and 14.6278 ×10-3 nmol carbonyl/ mg protein, respectively. Conclusions: Crude extracts of Xylaria sp. induced oxidative stress that may have caused the mortality of A. aegypti larvae. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 55 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Cytotoxicity Carbonyl Dengue TBARS Endophytic fungus Arctic medicine. Tropical medicine RC955-962 |
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Cytotoxicity Carbonyl Dengue TBARS Endophytic fungus Arctic medicine. Tropical medicine RC955-962 Maria Beatriz Silva Costa Rejane de Castro Simões Márcia de Jesus Amazonas da Silva André Correa de Oliveira Leonard Domingo Rosales Acho Emerson Silva Lima Wanderli Pedro Tadei Helder Lopes Teles Camila Martins de Oliveira Oxidative stress induction by crude extract of Xylaria sp. triggers lethality in the larvae of Aedes aegypti (Diptera: Culicidae) |
topic_facet |
Cytotoxicity Carbonyl Dengue TBARS Endophytic fungus Arctic medicine. Tropical medicine RC955-962 |
description |
ABSTRACT Background: Aedes aegypti is currently controlled with synthetic larvicides; however, mosquitoes have become highly resistant to these larvicides and difficult to eradicate. Studies have shown that insecticides derived from fungal extracts have various mechanisms of action that reduce the risk of resistance in these mosquitoes. One possible mechanism is uncontrolled production of reactive oxygen species (ROS) in the larvae, which can cause changes at the cellular level. Thus, the crude extract of Xylaria sp. was evaluated to investigate the oxidative effect of this extract in A. aegypti larvae by quantifying the oxidative damage to proteins and lipids. Methods: The larvicidal potential of the crude extract of Xylaria sp. Was evaluated, and the extract was subsequently tested in human lung fibroblasts for cytotoxicity and ROS production. ROS level was quantified in the larvae that were killed following exposure to the extract in the larvicide test. Results: The crude extract of Xylaria sp. Caused cytotoxicity and induced ROS production in human lung fibroblasts and A. aegypti larvae, respectively. In the larvicide trial, the extract showed an LC50 of 264.456 ppm and an LC90 of 364.307 ppm, and was thus considered active. The extract showed greater oxidative damage to lipids and proteins, with LC90 values of 24.7 µmol MDA/L and 14.6278 ×10-3 nmol carbonyl/ mg protein, respectively. Conclusions: Crude extracts of Xylaria sp. induced oxidative stress that may have caused the mortality of A. aegypti larvae. |
format |
Article in Journal/Newspaper |
author |
Maria Beatriz Silva Costa Rejane de Castro Simões Márcia de Jesus Amazonas da Silva André Correa de Oliveira Leonard Domingo Rosales Acho Emerson Silva Lima Wanderli Pedro Tadei Helder Lopes Teles Camila Martins de Oliveira |
author_facet |
Maria Beatriz Silva Costa Rejane de Castro Simões Márcia de Jesus Amazonas da Silva André Correa de Oliveira Leonard Domingo Rosales Acho Emerson Silva Lima Wanderli Pedro Tadei Helder Lopes Teles Camila Martins de Oliveira |
author_sort |
Maria Beatriz Silva Costa |
title |
Oxidative stress induction by crude extract of Xylaria sp. triggers lethality in the larvae of Aedes aegypti (Diptera: Culicidae) |
title_short |
Oxidative stress induction by crude extract of Xylaria sp. triggers lethality in the larvae of Aedes aegypti (Diptera: Culicidae) |
title_full |
Oxidative stress induction by crude extract of Xylaria sp. triggers lethality in the larvae of Aedes aegypti (Diptera: Culicidae) |
title_fullStr |
Oxidative stress induction by crude extract of Xylaria sp. triggers lethality in the larvae of Aedes aegypti (Diptera: Culicidae) |
title_full_unstemmed |
Oxidative stress induction by crude extract of Xylaria sp. triggers lethality in the larvae of Aedes aegypti (Diptera: Culicidae) |
title_sort |
oxidative stress induction by crude extract of xylaria sp. triggers lethality in the larvae of aedes aegypti (diptera: culicidae) |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2022 |
url |
https://doi.org/10.1590/0037-8682-0373-2021 https://doaj.org/article/04d91dd2867d4cf5b6675f3685b818e5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 55 (2022) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822022000100312&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0373-2021 https://doaj.org/article/04d91dd2867d4cf5b6675f3685b818e5 |
op_doi |
https://doi.org/10.1590/0037-8682-0373-2021 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
container_volume |
55 |
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