Neuroanatomical evidence of the transport of the rabies virus through the propriospinal tract in the spinal cord of mice
Introduction: Information about the neuroanatomical details of the ascendant transport of the rabies virus through the spinal cord is scarce. Objective: To identify the neuroanatomical route of dissemination of the rabies virus at each of the levels of the spinal cord of mice after being inoculated...
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Instituto Nacional de Salud
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ftdoajarticles:oai:doaj.org/article:04a77599898d4f73a7260004141615f0 2023-05-15T15:10:53+02:00 Neuroanatomical evidence of the transport of the rabies virus through the propriospinal tract in the spinal cord of mice Gerardo Santamaria Jeison Monroy-Gómez Orlando Torres-Fernández 2018-06-01T00:00:00Z https://doi.org/10.7705/biomedica.v38i0.3711 https://doaj.org/article/04a77599898d4f73a7260004141615f0 EN ES eng spa Instituto Nacional de Salud https://www.revistabiomedica.org/index.php/biomedica/article/view/3711 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v38i0.3711 https://doaj.org/article/04a77599898d4f73a7260004141615f0 Biomédica: revista del Instituto Nacional de Salud, Vol 38, Iss 2, Pp 209-215 (2018) virus de la rabia médula espinal transporte axonal inmunohistoquímica neuronas motoras ratón Medicine R Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.7705/biomedica.v38i0.3711 2022-12-30T23:47:58Z Introduction: Information about the neuroanatomical details of the ascendant transport of the rabies virus through the spinal cord is scarce. Objective: To identify the neuroanatomical route of dissemination of the rabies virus at each of the levels of the spinal cord of mice after being inoculated intramuscularly. Materials and methods: Mice were inoculated with the rabies virus in the hamstrings. After 24 hours post-inoculation, every eight hours, five animals were sacrificed by perfusion with paraformaldehyde. Then, the spinal cord was removed, and transverse cuts were made at the lumbosacral, thoracic, and cervical levels. These were processed by immunohistochemistry for the detection of viral antigens. Results: The first antigens of rabies were observed as aggregated particles in the lumbar spinal cord at 24 hours post-inoculation, within the ventral horn in the same side of the inoculated limb. At 32 hours post inoculation the first motoneurons immunoreactive to the virus became visible. At 40 hours postinoculation the first immunoreactive neurons were revealed in the thoracic level, located on lamina 8 and at 48 hours post-inoculation in the cervical cord, also on lamina 8. At 56 hours post-inoculation the virus had spread throughout the spinal cord, but the animals still did not show signs of the disease. Conclusion: In the mouse model we used, the rabies virus entered the spinal cord through the motoneurons and probably used the descending propriospinal pathway for its retrograde axonal transport to the encephalus. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Biomédica 38 2 209 215 |
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virus de la rabia médula espinal transporte axonal inmunohistoquímica neuronas motoras ratón Medicine R Arctic medicine. Tropical medicine RC955-962 |
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virus de la rabia médula espinal transporte axonal inmunohistoquímica neuronas motoras ratón Medicine R Arctic medicine. Tropical medicine RC955-962 Gerardo Santamaria Jeison Monroy-Gómez Orlando Torres-Fernández Neuroanatomical evidence of the transport of the rabies virus through the propriospinal tract in the spinal cord of mice |
topic_facet |
virus de la rabia médula espinal transporte axonal inmunohistoquímica neuronas motoras ratón Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction: Information about the neuroanatomical details of the ascendant transport of the rabies virus through the spinal cord is scarce. Objective: To identify the neuroanatomical route of dissemination of the rabies virus at each of the levels of the spinal cord of mice after being inoculated intramuscularly. Materials and methods: Mice were inoculated with the rabies virus in the hamstrings. After 24 hours post-inoculation, every eight hours, five animals were sacrificed by perfusion with paraformaldehyde. Then, the spinal cord was removed, and transverse cuts were made at the lumbosacral, thoracic, and cervical levels. These were processed by immunohistochemistry for the detection of viral antigens. Results: The first antigens of rabies were observed as aggregated particles in the lumbar spinal cord at 24 hours post-inoculation, within the ventral horn in the same side of the inoculated limb. At 32 hours post inoculation the first motoneurons immunoreactive to the virus became visible. At 40 hours postinoculation the first immunoreactive neurons were revealed in the thoracic level, located on lamina 8 and at 48 hours post-inoculation in the cervical cord, also on lamina 8. At 56 hours post-inoculation the virus had spread throughout the spinal cord, but the animals still did not show signs of the disease. Conclusion: In the mouse model we used, the rabies virus entered the spinal cord through the motoneurons and probably used the descending propriospinal pathway for its retrograde axonal transport to the encephalus. |
format |
Article in Journal/Newspaper |
author |
Gerardo Santamaria Jeison Monroy-Gómez Orlando Torres-Fernández |
author_facet |
Gerardo Santamaria Jeison Monroy-Gómez Orlando Torres-Fernández |
author_sort |
Gerardo Santamaria |
title |
Neuroanatomical evidence of the transport of the rabies virus through the propriospinal tract in the spinal cord of mice |
title_short |
Neuroanatomical evidence of the transport of the rabies virus through the propriospinal tract in the spinal cord of mice |
title_full |
Neuroanatomical evidence of the transport of the rabies virus through the propriospinal tract in the spinal cord of mice |
title_fullStr |
Neuroanatomical evidence of the transport of the rabies virus through the propriospinal tract in the spinal cord of mice |
title_full_unstemmed |
Neuroanatomical evidence of the transport of the rabies virus through the propriospinal tract in the spinal cord of mice |
title_sort |
neuroanatomical evidence of the transport of the rabies virus through the propriospinal tract in the spinal cord of mice |
publisher |
Instituto Nacional de Salud |
publishDate |
2018 |
url |
https://doi.org/10.7705/biomedica.v38i0.3711 https://doaj.org/article/04a77599898d4f73a7260004141615f0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 38, Iss 2, Pp 209-215 (2018) |
op_relation |
https://www.revistabiomedica.org/index.php/biomedica/article/view/3711 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v38i0.3711 https://doaj.org/article/04a77599898d4f73a7260004141615f0 |
op_doi |
https://doi.org/10.7705/biomedica.v38i0.3711 |
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Biomédica |
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38 |
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2 |
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209 |
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215 |
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