An improved extraction method reveals varied DNA content in different parts of the shells of Pacific oysters
The DNA in the shell of Crassostrea gigas could have important roles in the shell biomineralization. However, limited by the low efficiency of existing extraction methods, studies investigating the DNA in shells are lacking. In this study, the shell DNA of C. gigas was extracted using the organic so...
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ftdoajarticles:oai:doaj.org/article:04a4fc9537da493db42b4fc35bdd676f 2023-10-09T21:50:56+02:00 An improved extraction method reveals varied DNA content in different parts of the shells of Pacific oysters Jiang Qiuyun Wei Lei Gai Chaowei Yu Wenchao He Cheng Chen Man Zhang Zhen Song Hongce Wang Xiaona Wang Xiaotong 2019-01-01T00:00:00Z https://doi.org/10.1051/alr/2019003 https://doaj.org/article/04a4fc9537da493db42b4fc35bdd676f EN eng EDP Sciences https://www.alr-journal.org/articles/alr/full_html/2019/01/alr180106/alr180106.html https://doaj.org/toc/1765-2952 1765-2952 doi:10.1051/alr/2019003 https://doaj.org/article/04a4fc9537da493db42b4fc35bdd676f Aquatic Living Resources, Vol 32, p 5 (2019) pacific oyster shell dna in vivo sampling dna extraction shell growth Aquaculture. Fisheries. Angling SH1-691 article 2019 ftdoajarticles https://doi.org/10.1051/alr/2019003 2023-09-10T00:40:36Z The DNA in the shell of Crassostrea gigas could have important roles in the shell biomineralization. However, limited by the low efficiency of existing extraction methods, studies investigating the DNA in shells are lacking. In this study, the shell DNA of C. gigas was extracted using the organic solvent extraction (OSE) and guanidine lysis buffer (GLB) methods; the efficiency and quality of these two methods were compared. The sequences of a mitochondrial gene (cytochrome c oxidase subunit I, COI) and a nuclear gene (28S rRNA) of C. gigas were analyzed to verify the origin of the extracted shell DNA. Finally, the DNA contents of the ventral edge, middle part, and dorsal edge of C. gigas shells were compared. The results showed that OSE had a higher DNA extraction efficiency than GLB; the oyster shell DNA was homologous to the oyster genome; the DNA content was higher in the ventral edge than in the middle part or in the dorsal edge of the C. gigas shell. This study not only reports an improved extraction method for the mollusk shell DNA, but also revealed that the DNA in the oyster shell originates from the oyster body and that the DNA content in different parts of the C. gigas shell showed obvious variance. These results provide supporting evidence for the hypothesis that oyster cells participate in shell formation, and also afford a nondestructive method for oyster genetic identification, which can promote the application of molecular biology technology in oyster breeding. In addition, a shell growth pattern of ‘Under Old & Exceeding Old’ was also proposed. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Aquatic Living Resources 32 5 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
pacific oyster shell dna in vivo sampling dna extraction shell growth Aquaculture. Fisheries. Angling SH1-691 |
spellingShingle |
pacific oyster shell dna in vivo sampling dna extraction shell growth Aquaculture. Fisheries. Angling SH1-691 Jiang Qiuyun Wei Lei Gai Chaowei Yu Wenchao He Cheng Chen Man Zhang Zhen Song Hongce Wang Xiaona Wang Xiaotong An improved extraction method reveals varied DNA content in different parts of the shells of Pacific oysters |
topic_facet |
pacific oyster shell dna in vivo sampling dna extraction shell growth Aquaculture. Fisheries. Angling SH1-691 |
description |
The DNA in the shell of Crassostrea gigas could have important roles in the shell biomineralization. However, limited by the low efficiency of existing extraction methods, studies investigating the DNA in shells are lacking. In this study, the shell DNA of C. gigas was extracted using the organic solvent extraction (OSE) and guanidine lysis buffer (GLB) methods; the efficiency and quality of these two methods were compared. The sequences of a mitochondrial gene (cytochrome c oxidase subunit I, COI) and a nuclear gene (28S rRNA) of C. gigas were analyzed to verify the origin of the extracted shell DNA. Finally, the DNA contents of the ventral edge, middle part, and dorsal edge of C. gigas shells were compared. The results showed that OSE had a higher DNA extraction efficiency than GLB; the oyster shell DNA was homologous to the oyster genome; the DNA content was higher in the ventral edge than in the middle part or in the dorsal edge of the C. gigas shell. This study not only reports an improved extraction method for the mollusk shell DNA, but also revealed that the DNA in the oyster shell originates from the oyster body and that the DNA content in different parts of the C. gigas shell showed obvious variance. These results provide supporting evidence for the hypothesis that oyster cells participate in shell formation, and also afford a nondestructive method for oyster genetic identification, which can promote the application of molecular biology technology in oyster breeding. In addition, a shell growth pattern of ‘Under Old & Exceeding Old’ was also proposed. |
format |
Article in Journal/Newspaper |
author |
Jiang Qiuyun Wei Lei Gai Chaowei Yu Wenchao He Cheng Chen Man Zhang Zhen Song Hongce Wang Xiaona Wang Xiaotong |
author_facet |
Jiang Qiuyun Wei Lei Gai Chaowei Yu Wenchao He Cheng Chen Man Zhang Zhen Song Hongce Wang Xiaona Wang Xiaotong |
author_sort |
Jiang Qiuyun |
title |
An improved extraction method reveals varied DNA content in different parts of the shells of Pacific oysters |
title_short |
An improved extraction method reveals varied DNA content in different parts of the shells of Pacific oysters |
title_full |
An improved extraction method reveals varied DNA content in different parts of the shells of Pacific oysters |
title_fullStr |
An improved extraction method reveals varied DNA content in different parts of the shells of Pacific oysters |
title_full_unstemmed |
An improved extraction method reveals varied DNA content in different parts of the shells of Pacific oysters |
title_sort |
improved extraction method reveals varied dna content in different parts of the shells of pacific oysters |
publisher |
EDP Sciences |
publishDate |
2019 |
url |
https://doi.org/10.1051/alr/2019003 https://doaj.org/article/04a4fc9537da493db42b4fc35bdd676f |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Aquatic Living Resources, Vol 32, p 5 (2019) |
op_relation |
https://www.alr-journal.org/articles/alr/full_html/2019/01/alr180106/alr180106.html https://doaj.org/toc/1765-2952 1765-2952 doi:10.1051/alr/2019003 https://doaj.org/article/04a4fc9537da493db42b4fc35bdd676f |
op_doi |
https://doi.org/10.1051/alr/2019003 |
container_title |
Aquatic Living Resources |
container_volume |
32 |
container_start_page |
5 |
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1779314030708523008 |