Phytoplankton community structure in the Western Subarctic Gyre of the Pacific Ocean during summer determined by a combined approach of HPLC-pigment CHEMTAX and metabarcoding sequencing
The Western Subarctic Gyre (WSG) is a cyclonic upwelling gyre in the northwest subarctic Pacific, which is a region with a high concentration of nutrients but low chlorophyll. We investigated the community structure and spatial distribution of phytoplankton in this area by using HPLC-pigment CHEMTAX...
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Frontiers Media S.A.
2023
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Online Access: | https://doi.org/10.3389/fmars.2023.1116050 https://doaj.org/article/04a1d62c3fd34359b088db498fab73b1 |
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ftdoajarticles:oai:doaj.org/article:04a1d62c3fd34359b088db498fab73b1 2023-05-15T18:28:00+02:00 Phytoplankton community structure in the Western Subarctic Gyre of the Pacific Ocean during summer determined by a combined approach of HPLC-pigment CHEMTAX and metabarcoding sequencing Quandong Xin Xiaohan Qin Guannan Wu Xiaokun Ding Xinliang Wang Qingjing Hu Changkao Mu Yuqiu Wei Jufa Chen Tao Jiang 2023-01-01T00:00:00Z https://doi.org/10.3389/fmars.2023.1116050 https://doaj.org/article/04a1d62c3fd34359b088db498fab73b1 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmars.2023.1116050/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2023.1116050 https://doaj.org/article/04a1d62c3fd34359b088db498fab73b1 Frontiers in Marine Science, Vol 10 (2023) metabarcoding analysis pigments phytoplankton Western Subarctic Gyre metabarcoding analysis Western Subarctic Gyre Science Q General. Including nature conservation geographical distribution QH1-199.5 article 2023 ftdoajarticles https://doi.org/10.3389/fmars.2023.1116050 2023-01-29T01:27:51Z The Western Subarctic Gyre (WSG) is a cyclonic upwelling gyre in the northwest subarctic Pacific, which is a region with a high concentration of nutrients but low chlorophyll. We investigated the community structure and spatial distribution of phytoplankton in this area by using HPLC-pigment CHEMTAX (a chemotaxonomy program) and metabarcoding sequencing during the summer of 2021. The phytoplankton community showed significant differences between the two methods. The CHEMTAX analyses identified eight major marine phytoplankton assemblages. Cryptophytes were the major contributors (24.96%) to the total Chl a, followed by pelagophytes, prymnesiophytes, diatoms, and chlorophytes. The eukaryotic phytoplankton OTUs obtained by metabarcoding were categorized into 149 species in 96 genera of 6 major groups (diatoms, prymnesiophytes, pelagophytes, chlorophytes, cryptophytes, and dinoflagellates). Dinoflagellates were the most abundant group, accounting for 44.74% of the total OTUs obtained, followed by cryptophytes and pelagophytes. Sixteen out of the 97 identified species were annotated as harmful algal species, and Heterocapsa rotundata, Karlodinium veneficum, and Aureococcus anophagefferens were assigned to the abundant group (i.e., at least 0.1% of the total reads). Nutrients were more important in shaping the phytoplankton community than temperature and salinity. The 24 stations were divided into southern and northern regions along 44°N according to the k-means method, with the former being dominated by high Chl a and low nutrients. Although different phytoplankton assemblages analyzed by the two methods showed various relationships with environmental factors, a common feature was that the dinoflagellate proportion showed a significantly negative correlation with low nutrients and a positive correlation with Chl a. Article in Journal/Newspaper Subarctic Directory of Open Access Journals: DOAJ Articles Pacific Frontiers in Marine Science 10 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
metabarcoding analysis pigments phytoplankton Western Subarctic Gyre metabarcoding analysis Western Subarctic Gyre Science Q General. Including nature conservation geographical distribution QH1-199.5 |
spellingShingle |
metabarcoding analysis pigments phytoplankton Western Subarctic Gyre metabarcoding analysis Western Subarctic Gyre Science Q General. Including nature conservation geographical distribution QH1-199.5 Quandong Xin Xiaohan Qin Guannan Wu Xiaokun Ding Xinliang Wang Qingjing Hu Changkao Mu Yuqiu Wei Jufa Chen Tao Jiang Phytoplankton community structure in the Western Subarctic Gyre of the Pacific Ocean during summer determined by a combined approach of HPLC-pigment CHEMTAX and metabarcoding sequencing |
topic_facet |
metabarcoding analysis pigments phytoplankton Western Subarctic Gyre metabarcoding analysis Western Subarctic Gyre Science Q General. Including nature conservation geographical distribution QH1-199.5 |
description |
The Western Subarctic Gyre (WSG) is a cyclonic upwelling gyre in the northwest subarctic Pacific, which is a region with a high concentration of nutrients but low chlorophyll. We investigated the community structure and spatial distribution of phytoplankton in this area by using HPLC-pigment CHEMTAX (a chemotaxonomy program) and metabarcoding sequencing during the summer of 2021. The phytoplankton community showed significant differences between the two methods. The CHEMTAX analyses identified eight major marine phytoplankton assemblages. Cryptophytes were the major contributors (24.96%) to the total Chl a, followed by pelagophytes, prymnesiophytes, diatoms, and chlorophytes. The eukaryotic phytoplankton OTUs obtained by metabarcoding were categorized into 149 species in 96 genera of 6 major groups (diatoms, prymnesiophytes, pelagophytes, chlorophytes, cryptophytes, and dinoflagellates). Dinoflagellates were the most abundant group, accounting for 44.74% of the total OTUs obtained, followed by cryptophytes and pelagophytes. Sixteen out of the 97 identified species were annotated as harmful algal species, and Heterocapsa rotundata, Karlodinium veneficum, and Aureococcus anophagefferens were assigned to the abundant group (i.e., at least 0.1% of the total reads). Nutrients were more important in shaping the phytoplankton community than temperature and salinity. The 24 stations were divided into southern and northern regions along 44°N according to the k-means method, with the former being dominated by high Chl a and low nutrients. Although different phytoplankton assemblages analyzed by the two methods showed various relationships with environmental factors, a common feature was that the dinoflagellate proportion showed a significantly negative correlation with low nutrients and a positive correlation with Chl a. |
format |
Article in Journal/Newspaper |
author |
Quandong Xin Xiaohan Qin Guannan Wu Xiaokun Ding Xinliang Wang Qingjing Hu Changkao Mu Yuqiu Wei Jufa Chen Tao Jiang |
author_facet |
Quandong Xin Xiaohan Qin Guannan Wu Xiaokun Ding Xinliang Wang Qingjing Hu Changkao Mu Yuqiu Wei Jufa Chen Tao Jiang |
author_sort |
Quandong Xin |
title |
Phytoplankton community structure in the Western Subarctic Gyre of the Pacific Ocean during summer determined by a combined approach of HPLC-pigment CHEMTAX and metabarcoding sequencing |
title_short |
Phytoplankton community structure in the Western Subarctic Gyre of the Pacific Ocean during summer determined by a combined approach of HPLC-pigment CHEMTAX and metabarcoding sequencing |
title_full |
Phytoplankton community structure in the Western Subarctic Gyre of the Pacific Ocean during summer determined by a combined approach of HPLC-pigment CHEMTAX and metabarcoding sequencing |
title_fullStr |
Phytoplankton community structure in the Western Subarctic Gyre of the Pacific Ocean during summer determined by a combined approach of HPLC-pigment CHEMTAX and metabarcoding sequencing |
title_full_unstemmed |
Phytoplankton community structure in the Western Subarctic Gyre of the Pacific Ocean during summer determined by a combined approach of HPLC-pigment CHEMTAX and metabarcoding sequencing |
title_sort |
phytoplankton community structure in the western subarctic gyre of the pacific ocean during summer determined by a combined approach of hplc-pigment chemtax and metabarcoding sequencing |
publisher |
Frontiers Media S.A. |
publishDate |
2023 |
url |
https://doi.org/10.3389/fmars.2023.1116050 https://doaj.org/article/04a1d62c3fd34359b088db498fab73b1 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Subarctic |
genre_facet |
Subarctic |
op_source |
Frontiers in Marine Science, Vol 10 (2023) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmars.2023.1116050/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2023.1116050 https://doaj.org/article/04a1d62c3fd34359b088db498fab73b1 |
op_doi |
https://doi.org/10.3389/fmars.2023.1116050 |
container_title |
Frontiers in Marine Science |
container_volume |
10 |
_version_ |
1766210282014638080 |