Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice
Leishmaniasis, a zoonosis of worldwide distribution, presents a significant impact on immunosupressed patients. This study aimed to evaluate Leishmania chagasi infection in BALB/c mice immunosuppressed with dexamethasone. Spleen cells stimulated or not with L. chagasi were cultured for cytokine quan...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:047954b39525448cb4d38ac207b7b00c 2023-05-15T15:04:04+02:00 Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice JG Machado JL Hoffmann VLK Krause AV da Silva LA Dias-Melicio H Langoni 2010-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992010000100013 https://doaj.org/article/047954b39525448cb4d38ac207b7b00c EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100013 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992010000100013 1678-9199 https://doaj.org/article/047954b39525448cb4d38ac207b7b00c Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 1, Pp 131-146 (2010) visceral leishmaniasis immunosuppression immunopathology mice interleukins Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2010 ftdoajarticles https://doi.org/10.1590/S1678-91992010000100013 2022-12-31T00:58:07Z Leishmaniasis, a zoonosis of worldwide distribution, presents a significant impact on immunosupressed patients. This study aimed to evaluate Leishmania chagasi infection in BALB/c mice immunosuppressed with dexamethasone. Spleen cells stimulated or not with L. chagasi were cultured for cytokine quantification (IFN-γ, IL-2, IL-4 and IL-10) by sandwich ELISA. Parasite loads in the spleen and liver were determined by means of culture microtitration. Immunosuppressed groups showed statistically lower spleen weight and CD4-cell percentage in blood on the day of infection and produced Th1 and Th2 cytokines on other days of the study. The other infected groups, weather immunosupressed or not, also produced Th1 and Th2 cytokines. Parasite loads in the spleen and liver were not statistically different among the groups. It was concluded that L. chagasi infection was not affected by dexamethasone-induced immunosuppression, probably due the reversible effect of the treatment. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 16 1 131 146 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
visceral leishmaniasis immunosuppression immunopathology mice interleukins Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
visceral leishmaniasis immunosuppression immunopathology mice interleukins Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 JG Machado JL Hoffmann VLK Krause AV da Silva LA Dias-Melicio H Langoni Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
topic_facet |
visceral leishmaniasis immunosuppression immunopathology mice interleukins Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Leishmaniasis, a zoonosis of worldwide distribution, presents a significant impact on immunosupressed patients. This study aimed to evaluate Leishmania chagasi infection in BALB/c mice immunosuppressed with dexamethasone. Spleen cells stimulated or not with L. chagasi were cultured for cytokine quantification (IFN-γ, IL-2, IL-4 and IL-10) by sandwich ELISA. Parasite loads in the spleen and liver were determined by means of culture microtitration. Immunosuppressed groups showed statistically lower spleen weight and CD4-cell percentage in blood on the day of infection and produced Th1 and Th2 cytokines on other days of the study. The other infected groups, weather immunosupressed or not, also produced Th1 and Th2 cytokines. Parasite loads in the spleen and liver were not statistically different among the groups. It was concluded that L. chagasi infection was not affected by dexamethasone-induced immunosuppression, probably due the reversible effect of the treatment. |
format |
Article in Journal/Newspaper |
author |
JG Machado JL Hoffmann VLK Krause AV da Silva LA Dias-Melicio H Langoni |
author_facet |
JG Machado JL Hoffmann VLK Krause AV da Silva LA Dias-Melicio H Langoni |
author_sort |
JG Machado |
title |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
title_short |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
title_full |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
title_fullStr |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
title_full_unstemmed |
Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice |
title_sort |
cell-mediated immune response to leishmania chagasi experimental infection of balb/c immunosuppressed mice |
publisher |
SciELO |
publishDate |
2010 |
url |
https://doi.org/10.1590/S1678-91992010000100013 https://doaj.org/article/047954b39525448cb4d38ac207b7b00c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 1, Pp 131-146 (2010) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100013 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992010000100013 1678-9199 https://doaj.org/article/047954b39525448cb4d38ac207b7b00c |
op_doi |
https://doi.org/10.1590/S1678-91992010000100013 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
16 |
container_issue |
1 |
container_start_page |
131 |
op_container_end_page |
146 |
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1766335890041339904 |