Molecular analysis of Salmonella enteritidis isolates from the Caribbean by pulsed-field gel electrophoresis
Using pulsed-field gel electrophoresis (PFGE), between 1987 and 1996 we analyzed Salmonella enteritidis isolates from gastroenteritis cases in four Caribbean countries: Barbados, Saint Kitts and Nevis, Saint Lucia, and Trinidad and Tobago. We also determined the resistance of the isolates to 12 anti...
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ftdoajarticles:oai:doaj.org/article:04664d14e93c4e7da3885d83b7c29ac1 2023-05-15T15:10:17+02:00 Molecular analysis of Salmonella enteritidis isolates from the Caribbean by pulsed-field gel electrophoresis Abiodun Adesiyun Andrew Carson Kelly McAdoo Craig Bailey 2000-11-01T00:00:00Z https://doi.org/10.1590/s1020-49892000001000004 https://doaj.org/article/04664d14e93c4e7da3885d83b7c29ac1 EN ES PT eng spa por Pan American Health Organization http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49892000001000004&lng=en&tlng=en https://doaj.org/toc/1020-4989 1020-4989 doi:10.1590/s1020-49892000001000004 https://doaj.org/article/04664d14e93c4e7da3885d83b7c29ac1 Revista Panamericana de Salud Pública, Vol 8, Iss 5, Pp 342-347 (2000) Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2000 ftdoajarticles https://doi.org/10.1590/s1020-49892000001000004 2022-12-31T14:25:54Z Using pulsed-field gel electrophoresis (PFGE), between 1987 and 1996 we analyzed Salmonella enteritidis isolates from gastroenteritis cases in four Caribbean countries: Barbados, Saint Kitts and Nevis, Saint Lucia, and Trinidad and Tobago. We also determined the resistance of the isolates to 12 antimicrobial agents. Of the 129 isolates of S. enteritidis available for testing, DNA digested by XbaI revealed 13 distinctive PFGE patterns. The most prevalent XbaI PFGE patterns were group 1 (88 of 129 isolates, 68.2%) and group 2 (26 of 129, 20.2%). The patterns found among S. enteritidis isolates correlated with the geographical origin of the isolates. Of the 28 isolates from Barbados, 20 of them (71.4%) belonged to XbaI PFGE group 2, and of the 93 isolates from Trinidad and Tobago, 78 of them (83.9%) belonged to group 1. SpeI digestion of S. enteritidis genome was not as discriminatory as XbaI. Overall, of the 129 isolates, 67 of them (51.9%) exhibited resistance to one or more of the 12 antimicrobial agents that we tested. The prevalence of resistance was 53.8% for the S. enteritidis isolates tested from Trinidad and Tobago, 50.0% for those from Barbados, 28.6% for those from Saint Lucia, and 100.0% for one isolate from the island of Saint Kitts. Resistance was highest to triple sulfur (59 of 129 isolates, 45.7%), followed by furadantoin (10 of 129, 7.8%), ampicillin (7 of 129, 5.4%), and carbamycin (5 of 129, 3.9%). Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Trinidad ENVELOPE(-60.734,-60.734,-63.816,-63.816) Revista Panamericana de Salud Pública 8 5 342 347 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English Spanish Portuguese |
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Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Abiodun Adesiyun Andrew Carson Kelly McAdoo Craig Bailey Molecular analysis of Salmonella enteritidis isolates from the Caribbean by pulsed-field gel electrophoresis |
topic_facet |
Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Using pulsed-field gel electrophoresis (PFGE), between 1987 and 1996 we analyzed Salmonella enteritidis isolates from gastroenteritis cases in four Caribbean countries: Barbados, Saint Kitts and Nevis, Saint Lucia, and Trinidad and Tobago. We also determined the resistance of the isolates to 12 antimicrobial agents. Of the 129 isolates of S. enteritidis available for testing, DNA digested by XbaI revealed 13 distinctive PFGE patterns. The most prevalent XbaI PFGE patterns were group 1 (88 of 129 isolates, 68.2%) and group 2 (26 of 129, 20.2%). The patterns found among S. enteritidis isolates correlated with the geographical origin of the isolates. Of the 28 isolates from Barbados, 20 of them (71.4%) belonged to XbaI PFGE group 2, and of the 93 isolates from Trinidad and Tobago, 78 of them (83.9%) belonged to group 1. SpeI digestion of S. enteritidis genome was not as discriminatory as XbaI. Overall, of the 129 isolates, 67 of them (51.9%) exhibited resistance to one or more of the 12 antimicrobial agents that we tested. The prevalence of resistance was 53.8% for the S. enteritidis isolates tested from Trinidad and Tobago, 50.0% for those from Barbados, 28.6% for those from Saint Lucia, and 100.0% for one isolate from the island of Saint Kitts. Resistance was highest to triple sulfur (59 of 129 isolates, 45.7%), followed by furadantoin (10 of 129, 7.8%), ampicillin (7 of 129, 5.4%), and carbamycin (5 of 129, 3.9%). |
format |
Article in Journal/Newspaper |
author |
Abiodun Adesiyun Andrew Carson Kelly McAdoo Craig Bailey |
author_facet |
Abiodun Adesiyun Andrew Carson Kelly McAdoo Craig Bailey |
author_sort |
Abiodun Adesiyun |
title |
Molecular analysis of Salmonella enteritidis isolates from the Caribbean by pulsed-field gel electrophoresis |
title_short |
Molecular analysis of Salmonella enteritidis isolates from the Caribbean by pulsed-field gel electrophoresis |
title_full |
Molecular analysis of Salmonella enteritidis isolates from the Caribbean by pulsed-field gel electrophoresis |
title_fullStr |
Molecular analysis of Salmonella enteritidis isolates from the Caribbean by pulsed-field gel electrophoresis |
title_full_unstemmed |
Molecular analysis of Salmonella enteritidis isolates from the Caribbean by pulsed-field gel electrophoresis |
title_sort |
molecular analysis of salmonella enteritidis isolates from the caribbean by pulsed-field gel electrophoresis |
publisher |
Pan American Health Organization |
publishDate |
2000 |
url |
https://doi.org/10.1590/s1020-49892000001000004 https://doaj.org/article/04664d14e93c4e7da3885d83b7c29ac1 |
long_lat |
ENVELOPE(-60.734,-60.734,-63.816,-63.816) |
geographic |
Arctic Trinidad |
geographic_facet |
Arctic Trinidad |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista Panamericana de Salud Pública, Vol 8, Iss 5, Pp 342-347 (2000) |
op_relation |
http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49892000001000004&lng=en&tlng=en https://doaj.org/toc/1020-4989 1020-4989 doi:10.1590/s1020-49892000001000004 https://doaj.org/article/04664d14e93c4e7da3885d83b7c29ac1 |
op_doi |
https://doi.org/10.1590/s1020-49892000001000004 |
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Revista Panamericana de Salud Pública |
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