Transfected Babesia bovis Expressing a Tick GST as a Live Vector Vaccine.
The Rhipicephalus microplus tick is a notorious blood-feeding ectoparasite of livestock, especially cattle, responsible for massive losses in animal production. It is the main vector for transmission of pathogenic bacteria and parasites, including Babesia bovis, an intraerythrocytic apicomplexan pro...
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ftdoajarticles:oai:doaj.org/article:0461ff1ebc1d4131a5e9f4b1040c413f 2023-05-15T15:14:07+02:00 Transfected Babesia bovis Expressing a Tick GST as a Live Vector Vaccine. Daiane P Oldiges Jacob M Laughery Nelson Junior Tagliari Ronaldo Viana Leite Filho William C Davis Itabajara da Silva Vaz Carlos Termignoni Donald P Knowles Carlos E Suarez 2016-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005152 https://doaj.org/article/0461ff1ebc1d4131a5e9f4b1040c413f EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5135042?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005152 https://doaj.org/article/0461ff1ebc1d4131a5e9f4b1040c413f PLoS Neglected Tropical Diseases, Vol 10, Iss 12, p e0005152 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005152 2022-12-31T12:36:25Z The Rhipicephalus microplus tick is a notorious blood-feeding ectoparasite of livestock, especially cattle, responsible for massive losses in animal production. It is the main vector for transmission of pathogenic bacteria and parasites, including Babesia bovis, an intraerythrocytic apicomplexan protozoan parasite responsible for bovine Babesiosis. This study describes the development and testing of a live B. bovis vaccine expressing the protective tick antigen glutathione-S-transferase from Haemaphysalis longicornis (HlGST). The B. bovis S74-T3B parasites were electroporated with a plasmid containing the bidirectional Ef-1α (elongation factor 1 alpha) promoter of B. bovis controlling expression of two independent genes, the selectable marker GFP-BSD (green fluorescent protein-blasticidin deaminase), and HlGST fused to the MSA-1 (merozoite surface antigen 1) signal peptide from B. bovis. Electroporation followed by blasticidin selection resulted in the emergence of a mixed B. bovis transfected line (termed HlGST) in in vitro cultures, containing parasites with distinct patterns of insertion of both exogenous genes, either in or outside the Ef-1α locus. A B. bovis clonal line termed HlGST-Cln expressing intracellular GFP and HlGST in the surface of merozoites was then derived from the mixed parasite line HlGST using a fluorescent activated cell sorter. Two independent calf immunization trials were performed via intravenous inoculation of the HlGST-Cln and a previously described control consisting of an irrelevant transfected clonal line of B. bovis designated GFP-Cln. The control GFP-Cln line contains a copy of the GFP-BSD gene inserted into the Ef-1α locus of B. bovis in an identical fashion as the HIGST-Cln parasites. All animals inoculated with the HlGST-Cln and GFP-Cln transfected parasites developed mild babesiosis. Tick egg fertility and fully engorged female tick weight was reduced significantly in R. microplus feeding on HlGST-Cln-immunized calves. Collectively, these data show the efficacy of a ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 12 e0005152 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Daiane P Oldiges Jacob M Laughery Nelson Junior Tagliari Ronaldo Viana Leite Filho William C Davis Itabajara da Silva Vaz Carlos Termignoni Donald P Knowles Carlos E Suarez Transfected Babesia bovis Expressing a Tick GST as a Live Vector Vaccine. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The Rhipicephalus microplus tick is a notorious blood-feeding ectoparasite of livestock, especially cattle, responsible for massive losses in animal production. It is the main vector for transmission of pathogenic bacteria and parasites, including Babesia bovis, an intraerythrocytic apicomplexan protozoan parasite responsible for bovine Babesiosis. This study describes the development and testing of a live B. bovis vaccine expressing the protective tick antigen glutathione-S-transferase from Haemaphysalis longicornis (HlGST). The B. bovis S74-T3B parasites were electroporated with a plasmid containing the bidirectional Ef-1α (elongation factor 1 alpha) promoter of B. bovis controlling expression of two independent genes, the selectable marker GFP-BSD (green fluorescent protein-blasticidin deaminase), and HlGST fused to the MSA-1 (merozoite surface antigen 1) signal peptide from B. bovis. Electroporation followed by blasticidin selection resulted in the emergence of a mixed B. bovis transfected line (termed HlGST) in in vitro cultures, containing parasites with distinct patterns of insertion of both exogenous genes, either in or outside the Ef-1α locus. A B. bovis clonal line termed HlGST-Cln expressing intracellular GFP and HlGST in the surface of merozoites was then derived from the mixed parasite line HlGST using a fluorescent activated cell sorter. Two independent calf immunization trials were performed via intravenous inoculation of the HlGST-Cln and a previously described control consisting of an irrelevant transfected clonal line of B. bovis designated GFP-Cln. The control GFP-Cln line contains a copy of the GFP-BSD gene inserted into the Ef-1α locus of B. bovis in an identical fashion as the HIGST-Cln parasites. All animals inoculated with the HlGST-Cln and GFP-Cln transfected parasites developed mild babesiosis. Tick egg fertility and fully engorged female tick weight was reduced significantly in R. microplus feeding on HlGST-Cln-immunized calves. Collectively, these data show the efficacy of a ... |
format |
Article in Journal/Newspaper |
author |
Daiane P Oldiges Jacob M Laughery Nelson Junior Tagliari Ronaldo Viana Leite Filho William C Davis Itabajara da Silva Vaz Carlos Termignoni Donald P Knowles Carlos E Suarez |
author_facet |
Daiane P Oldiges Jacob M Laughery Nelson Junior Tagliari Ronaldo Viana Leite Filho William C Davis Itabajara da Silva Vaz Carlos Termignoni Donald P Knowles Carlos E Suarez |
author_sort |
Daiane P Oldiges |
title |
Transfected Babesia bovis Expressing a Tick GST as a Live Vector Vaccine. |
title_short |
Transfected Babesia bovis Expressing a Tick GST as a Live Vector Vaccine. |
title_full |
Transfected Babesia bovis Expressing a Tick GST as a Live Vector Vaccine. |
title_fullStr |
Transfected Babesia bovis Expressing a Tick GST as a Live Vector Vaccine. |
title_full_unstemmed |
Transfected Babesia bovis Expressing a Tick GST as a Live Vector Vaccine. |
title_sort |
transfected babesia bovis expressing a tick gst as a live vector vaccine. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0005152 https://doaj.org/article/0461ff1ebc1d4131a5e9f4b1040c413f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 12, p e0005152 (2016) |
op_relation |
http://europepmc.org/articles/PMC5135042?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005152 https://doaj.org/article/0461ff1ebc1d4131a5e9f4b1040c413f |
op_doi |
https://doi.org/10.1371/journal.pntd.0005152 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
10 |
container_issue |
12 |
container_start_page |
e0005152 |
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1766344617837461504 |