Comparison of protein patterns after two-dimensional gel electrophoresis from leaves of in vitro cultures and seedlings of Rubus chamaemorus L.

• Published in: Acta Societatis Botanicorum Poloniae
• Main Authors: Barbara Thiem, Andrzej Kalinowski
• Format: Article in Journal/Newspaper
• Language: English
• Published: Polish Botanical Society 2014
• Subjects: Rubus chamaemorus; in vitro culture; shoot culture; protein; two-dimensional electrophoresis; Botany; QK1-989
• Online Access: https://doi.org/10.5586/asbp.2002.035; https://doaj.org/article/045c2fb0b9644946a9286b9e2e0d72e7

Proteins from leaves of Rubus chamaemorus propagated in vitro were subjected to miniaturized 2-D electrophoresis. The 2-DE patterns of proteins showed qualitative differences between plants propagated in vitro and control seedlings. More proteins of a high molecular weight were observed in leaves of plants from in vitro culture. A two-dimensional map of proteins from leaves provides detailed data concerning both polymorphism and protein patterns of this species. This makes it possible to start constructing a protein map of R. chamaemorus. The reasons for qualitative differences are discussed.