Comparison of protein patterns after two-dimensional gel electrophoresis from leaves of in vitro cultures and seedlings of Rubus chamaemorus L.

Proteins from leaves of Rubus chamaemorus propagated in vitro were subjected to miniaturized 2-D electrophoresis. The 2-DE patterns of proteins showed qualitative differences between plants propagated in vitro and control seedlings. More proteins of a high molecular weight were observed in leaves of...

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Bibliographic Details
Published in:Acta Societatis Botanicorum Poloniae
Main Authors: Barbara Thiem, Andrzej Kalinowski
Format: Article in Journal/Newspaper
Language:English
Published: Polish Botanical Society 2014
Subjects:
Online Access:https://doi.org/10.5586/asbp.2002.035
https://doaj.org/article/045c2fb0b9644946a9286b9e2e0d72e7
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Summary:Proteins from leaves of Rubus chamaemorus propagated in vitro were subjected to miniaturized 2-D electrophoresis. The 2-DE patterns of proteins showed qualitative differences between plants propagated in vitro and control seedlings. More proteins of a high molecular weight were observed in leaves of plants from in vitro culture. A two-dimensional map of proteins from leaves provides detailed data concerning both polymorphism and protein patterns of this species. This makes it possible to start constructing a protein map of R. chamaemorus. The reasons for qualitative differences are discussed.