Comparison of protein patterns after two-dimensional gel electrophoresis from leaves of in vitro cultures and seedlings of Rubus chamaemorus L.
Proteins from leaves of Rubus chamaemorus propagated in vitro were subjected to miniaturized 2-D electrophoresis. The 2-DE patterns of proteins showed qualitative differences between plants propagated in vitro and control seedlings. More proteins of a high molecular weight were observed in leaves of...
Published in: | Acta Societatis Botanicorum Poloniae |
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Main Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Polish Botanical Society
2014
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Subjects: | |
Online Access: | https://doi.org/10.5586/asbp.2002.035 https://doaj.org/article/045c2fb0b9644946a9286b9e2e0d72e7 |
Summary: | Proteins from leaves of Rubus chamaemorus propagated in vitro were subjected to miniaturized 2-D electrophoresis. The 2-DE patterns of proteins showed qualitative differences between plants propagated in vitro and control seedlings. More proteins of a high molecular weight were observed in leaves of plants from in vitro culture. A two-dimensional map of proteins from leaves provides detailed data concerning both polymorphism and protein patterns of this species. This makes it possible to start constructing a protein map of R. chamaemorus. The reasons for qualitative differences are discussed. |
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