Hydrophilic-treated plastic plates for wide-range analysis of Giemsa-stained red blood cells and automated Plasmodium infection rate counting
Abstract Background Malaria is a red blood cell (RBC) infection caused by Plasmodium parasites. To determine RBC infection rate, which is essential for malaria study and diagnosis, microscopic evaluation of Giemsa-stained thin blood smears on glass slides (‘Giemsa microscopy’) has been performed as...
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ftdoajarticles:oai:doaj.org/article:0458a9cf8c7041798565bfd37ad5581d 2023-05-15T15:13:11+02:00 Hydrophilic-treated plastic plates for wide-range analysis of Giemsa-stained red blood cells and automated Plasmodium infection rate counting Muneaki Hashimoto Shouki Yatsushiro Shohei Yamamura Masato Tanaka Hirokazu Sakamoto Yusuke Ido Kazuaki Kajimoto Mika Bando Jun-ichi Kido Masatoshi Kataoka 2017-08-01T00:00:00Z https://doi.org/10.1186/s12936-017-1975-9 https://doaj.org/article/0458a9cf8c7041798565bfd37ad5581d EN eng BMC http://link.springer.com/article/10.1186/s12936-017-1975-9 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-1975-9 1475-2875 https://doaj.org/article/0458a9cf8c7041798565bfd37ad5581d Malaria Journal, Vol 16, Iss 1, Pp 1-10 (2017) Malaria Diagnosis Automation Hydrophilic treatment Giemsa-staining Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2017 ftdoajarticles https://doi.org/10.1186/s12936-017-1975-9 2022-12-31T00:14:03Z Abstract Background Malaria is a red blood cell (RBC) infection caused by Plasmodium parasites. To determine RBC infection rate, which is essential for malaria study and diagnosis, microscopic evaluation of Giemsa-stained thin blood smears on glass slides (‘Giemsa microscopy’) has been performed as the accepted gold standard for over 100 years. However, only a small area of the blood smear provides a monolayer of RBCs suitable for determination of infection rate, which is one of the major reasons for the low parasite detection rate by Giemsa microscopy. In addition, because Giemsa microscopy is exacting and time-consuming, automated counting of infection rates is highly desirable. Results A method that allows for microscopic examination of Giemsa-stained cells spread in a monolayer on almost the whole surface of hydrophilic-treated cyclic olefin copolymer (COC) plates was established. Because wide-range Giemsa microscopy can be performed on a hydrophilic-treated plate, the method may enable more reliable diagnosis of malaria in patients with low parasitaemia burden. Furthermore, the number of RBCs and parasites stained with a fluorescent nuclear staining dye could be counted automatically with a software tool, without Giemsa staining. As a result, researchers studying malaria may calculate the infection rate easily, rapidly, and accurately even in low parasitaemia. Conclusion Because the running cost of these methods is very low and they do not involve complicated techniques, the use of hydrophilic COC plates may contribute to improved and more accurate diagnosis and research of malaria. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 16 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Malaria Diagnosis Automation Hydrophilic treatment Giemsa-staining Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria Diagnosis Automation Hydrophilic treatment Giemsa-staining Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Muneaki Hashimoto Shouki Yatsushiro Shohei Yamamura Masato Tanaka Hirokazu Sakamoto Yusuke Ido Kazuaki Kajimoto Mika Bando Jun-ichi Kido Masatoshi Kataoka Hydrophilic-treated plastic plates for wide-range analysis of Giemsa-stained red blood cells and automated Plasmodium infection rate counting |
topic_facet |
Malaria Diagnosis Automation Hydrophilic treatment Giemsa-staining Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Malaria is a red blood cell (RBC) infection caused by Plasmodium parasites. To determine RBC infection rate, which is essential for malaria study and diagnosis, microscopic evaluation of Giemsa-stained thin blood smears on glass slides (‘Giemsa microscopy’) has been performed as the accepted gold standard for over 100 years. However, only a small area of the blood smear provides a monolayer of RBCs suitable for determination of infection rate, which is one of the major reasons for the low parasite detection rate by Giemsa microscopy. In addition, because Giemsa microscopy is exacting and time-consuming, automated counting of infection rates is highly desirable. Results A method that allows for microscopic examination of Giemsa-stained cells spread in a monolayer on almost the whole surface of hydrophilic-treated cyclic olefin copolymer (COC) plates was established. Because wide-range Giemsa microscopy can be performed on a hydrophilic-treated plate, the method may enable more reliable diagnosis of malaria in patients with low parasitaemia burden. Furthermore, the number of RBCs and parasites stained with a fluorescent nuclear staining dye could be counted automatically with a software tool, without Giemsa staining. As a result, researchers studying malaria may calculate the infection rate easily, rapidly, and accurately even in low parasitaemia. Conclusion Because the running cost of these methods is very low and they do not involve complicated techniques, the use of hydrophilic COC plates may contribute to improved and more accurate diagnosis and research of malaria. |
format |
Article in Journal/Newspaper |
author |
Muneaki Hashimoto Shouki Yatsushiro Shohei Yamamura Masato Tanaka Hirokazu Sakamoto Yusuke Ido Kazuaki Kajimoto Mika Bando Jun-ichi Kido Masatoshi Kataoka |
author_facet |
Muneaki Hashimoto Shouki Yatsushiro Shohei Yamamura Masato Tanaka Hirokazu Sakamoto Yusuke Ido Kazuaki Kajimoto Mika Bando Jun-ichi Kido Masatoshi Kataoka |
author_sort |
Muneaki Hashimoto |
title |
Hydrophilic-treated plastic plates for wide-range analysis of Giemsa-stained red blood cells and automated Plasmodium infection rate counting |
title_short |
Hydrophilic-treated plastic plates for wide-range analysis of Giemsa-stained red blood cells and automated Plasmodium infection rate counting |
title_full |
Hydrophilic-treated plastic plates for wide-range analysis of Giemsa-stained red blood cells and automated Plasmodium infection rate counting |
title_fullStr |
Hydrophilic-treated plastic plates for wide-range analysis of Giemsa-stained red blood cells and automated Plasmodium infection rate counting |
title_full_unstemmed |
Hydrophilic-treated plastic plates for wide-range analysis of Giemsa-stained red blood cells and automated Plasmodium infection rate counting |
title_sort |
hydrophilic-treated plastic plates for wide-range analysis of giemsa-stained red blood cells and automated plasmodium infection rate counting |
publisher |
BMC |
publishDate |
2017 |
url |
https://doi.org/10.1186/s12936-017-1975-9 https://doaj.org/article/0458a9cf8c7041798565bfd37ad5581d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 16, Iss 1, Pp 1-10 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s12936-017-1975-9 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-1975-9 1475-2875 https://doaj.org/article/0458a9cf8c7041798565bfd37ad5581d |
op_doi |
https://doi.org/10.1186/s12936-017-1975-9 |
container_title |
Malaria Journal |
container_volume |
16 |
container_issue |
1 |
_version_ |
1766343774368169984 |