Sub-microscopic Plasmodium falciparum parasitaemia, dihydropteroate synthase (dhps) resistance mutations to sulfadoxine–pyrimethamine, transmission intensity and risk of malaria infection in pregnancy in Mount Cameroon Region
Abstract Background Plasmodium falciparum resistance to intermittent preventive treatment with sulfadoxine-pyrimethamine (IPTp-SP) continues to spread throughout sub-Saharan Africa. This study assessed the occurrence of microscopic and sub-microscopic P. falciparum parasitaemia, dihydropteroate synt...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2023
|
Subjects: | |
Online Access: | https://doi.org/10.1186/s12936-023-04485-7 https://doaj.org/article/0406446ade9445a99df85c19286a6111 |
id |
ftdoajarticles:oai:doaj.org/article:0406446ade9445a99df85c19286a6111 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:0406446ade9445a99df85c19286a6111 2023-05-15T15:18:22+02:00 Sub-microscopic Plasmodium falciparum parasitaemia, dihydropteroate synthase (dhps) resistance mutations to sulfadoxine–pyrimethamine, transmission intensity and risk of malaria infection in pregnancy in Mount Cameroon Region Harry F. Mbacham Diange M Mosume Tobias O. Apinjoh Vincent N. Ntui Marcel N. Moyeh Laken N. Kalaji Godlove B. Wepnje Stephen M Ghogomu Jodie A Dionne Alan T.N. Tita Eric A. Achidi Judith K. Anchang-Kimbi 2023-03-01T00:00:00Z https://doi.org/10.1186/s12936-023-04485-7 https://doaj.org/article/0406446ade9445a99df85c19286a6111 EN eng BMC https://doi.org/10.1186/s12936-023-04485-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-023-04485-7 1475-2875 https://doaj.org/article/0406446ade9445a99df85c19286a6111 Malaria Journal, Vol 22, Iss 1, Pp 1-16 (2023) Pregnancy IPTp-SP P. falciparum Sub-microscopic parasitaemia Dhps resistant mutations Cameroon Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2023 ftdoajarticles https://doi.org/10.1186/s12936-023-04485-7 2023-03-26T01:33:52Z Abstract Background Plasmodium falciparum resistance to intermittent preventive treatment with sulfadoxine-pyrimethamine (IPTp-SP) continues to spread throughout sub-Saharan Africa. This study assessed the occurrence of microscopic and sub-microscopic P. falciparum parasitaemia, dihydropteroate synthase mutations associated with resistance to SP and maternal anaemia in the Mount Cameroon area. Methods Consenting pregnant women living in semi-rural and semi-urban/urbanized settings were enrolled in this cross-sectional study. Socio-demographic, antenatal and clinical data were documented. Microscopic and sub-microscopic parasitaemia were diagnosed using peripheral blood microscopy and nested polymerase chain reaction (PCR) respectively. The dhps mutations were genotyped by restriction fragment length polymorphism analysis. The presence of A437G, K540E, and A581G was considered a marker for high-level resistance. Haemoglobin levels and anaemia status were determined. Results Among the women, the prevalence of microscopic and sub-microscopic P. falciparum infection were 7.7% (67/874) and 18.6% (93/500) respectively. Predictors of microscopic infection were younger age (< 21 years) (AOR = 2.89; 95% CI 1.29–6.46) and semi-rural settings (AOR = 2.27; 95% CI 1.31–3.96). Determinants of sub-microscopic infection were the rainy season (AOR, 3.01; 95% CI 1.77–5.13), primigravidity (AOR = 0.45; 95% CI 0.21–0.94) and regular ITN usage (AOR = 0.49; 95% CI 0.27–0.90). Of the145 P. falciparum isolates genotyped, 66.9% (97) carried mutations associated with resistance to SP; 33.8% (49), 0%, 52.4% (76) and 19.3% (28) for A437G, K540E, A581G and A437G + A581G respectively. The A581G mutation was associated with ≥ 3 SP doses evident only among sub-microscopic parasitaemia (P = 0.027) and multigravidae (P = 0.009). Women with microscopic infection were more likely from semi-rural settings (AOR = 7.09; 95% CI 2.59–19.42), to report history of fever (AOR = 2.6; 95% CI 1.07–6.31), to harbour parasites with double resistant ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 22 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Pregnancy IPTp-SP P. falciparum Sub-microscopic parasitaemia Dhps resistant mutations Cameroon Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Pregnancy IPTp-SP P. falciparum Sub-microscopic parasitaemia Dhps resistant mutations Cameroon Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Harry F. Mbacham Diange M Mosume Tobias O. Apinjoh Vincent N. Ntui Marcel N. Moyeh Laken N. Kalaji Godlove B. Wepnje Stephen M Ghogomu Jodie A Dionne Alan T.N. Tita Eric A. Achidi Judith K. Anchang-Kimbi Sub-microscopic Plasmodium falciparum parasitaemia, dihydropteroate synthase (dhps) resistance mutations to sulfadoxine–pyrimethamine, transmission intensity and risk of malaria infection in pregnancy in Mount Cameroon Region |
topic_facet |
Pregnancy IPTp-SP P. falciparum Sub-microscopic parasitaemia Dhps resistant mutations Cameroon Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Plasmodium falciparum resistance to intermittent preventive treatment with sulfadoxine-pyrimethamine (IPTp-SP) continues to spread throughout sub-Saharan Africa. This study assessed the occurrence of microscopic and sub-microscopic P. falciparum parasitaemia, dihydropteroate synthase mutations associated with resistance to SP and maternal anaemia in the Mount Cameroon area. Methods Consenting pregnant women living in semi-rural and semi-urban/urbanized settings were enrolled in this cross-sectional study. Socio-demographic, antenatal and clinical data were documented. Microscopic and sub-microscopic parasitaemia were diagnosed using peripheral blood microscopy and nested polymerase chain reaction (PCR) respectively. The dhps mutations were genotyped by restriction fragment length polymorphism analysis. The presence of A437G, K540E, and A581G was considered a marker for high-level resistance. Haemoglobin levels and anaemia status were determined. Results Among the women, the prevalence of microscopic and sub-microscopic P. falciparum infection were 7.7% (67/874) and 18.6% (93/500) respectively. Predictors of microscopic infection were younger age (< 21 years) (AOR = 2.89; 95% CI 1.29–6.46) and semi-rural settings (AOR = 2.27; 95% CI 1.31–3.96). Determinants of sub-microscopic infection were the rainy season (AOR, 3.01; 95% CI 1.77–5.13), primigravidity (AOR = 0.45; 95% CI 0.21–0.94) and regular ITN usage (AOR = 0.49; 95% CI 0.27–0.90). Of the145 P. falciparum isolates genotyped, 66.9% (97) carried mutations associated with resistance to SP; 33.8% (49), 0%, 52.4% (76) and 19.3% (28) for A437G, K540E, A581G and A437G + A581G respectively. The A581G mutation was associated with ≥ 3 SP doses evident only among sub-microscopic parasitaemia (P = 0.027) and multigravidae (P = 0.009). Women with microscopic infection were more likely from semi-rural settings (AOR = 7.09; 95% CI 2.59–19.42), to report history of fever (AOR = 2.6; 95% CI 1.07–6.31), to harbour parasites with double resistant ... |
format |
Article in Journal/Newspaper |
author |
Harry F. Mbacham Diange M Mosume Tobias O. Apinjoh Vincent N. Ntui Marcel N. Moyeh Laken N. Kalaji Godlove B. Wepnje Stephen M Ghogomu Jodie A Dionne Alan T.N. Tita Eric A. Achidi Judith K. Anchang-Kimbi |
author_facet |
Harry F. Mbacham Diange M Mosume Tobias O. Apinjoh Vincent N. Ntui Marcel N. Moyeh Laken N. Kalaji Godlove B. Wepnje Stephen M Ghogomu Jodie A Dionne Alan T.N. Tita Eric A. Achidi Judith K. Anchang-Kimbi |
author_sort |
Harry F. Mbacham |
title |
Sub-microscopic Plasmodium falciparum parasitaemia, dihydropteroate synthase (dhps) resistance mutations to sulfadoxine–pyrimethamine, transmission intensity and risk of malaria infection in pregnancy in Mount Cameroon Region |
title_short |
Sub-microscopic Plasmodium falciparum parasitaemia, dihydropteroate synthase (dhps) resistance mutations to sulfadoxine–pyrimethamine, transmission intensity and risk of malaria infection in pregnancy in Mount Cameroon Region |
title_full |
Sub-microscopic Plasmodium falciparum parasitaemia, dihydropteroate synthase (dhps) resistance mutations to sulfadoxine–pyrimethamine, transmission intensity and risk of malaria infection in pregnancy in Mount Cameroon Region |
title_fullStr |
Sub-microscopic Plasmodium falciparum parasitaemia, dihydropteroate synthase (dhps) resistance mutations to sulfadoxine–pyrimethamine, transmission intensity and risk of malaria infection in pregnancy in Mount Cameroon Region |
title_full_unstemmed |
Sub-microscopic Plasmodium falciparum parasitaemia, dihydropteroate synthase (dhps) resistance mutations to sulfadoxine–pyrimethamine, transmission intensity and risk of malaria infection in pregnancy in Mount Cameroon Region |
title_sort |
sub-microscopic plasmodium falciparum parasitaemia, dihydropteroate synthase (dhps) resistance mutations to sulfadoxine–pyrimethamine, transmission intensity and risk of malaria infection in pregnancy in mount cameroon region |
publisher |
BMC |
publishDate |
2023 |
url |
https://doi.org/10.1186/s12936-023-04485-7 https://doaj.org/article/0406446ade9445a99df85c19286a6111 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 22, Iss 1, Pp 1-16 (2023) |
op_relation |
https://doi.org/10.1186/s12936-023-04485-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-023-04485-7 1475-2875 https://doaj.org/article/0406446ade9445a99df85c19286a6111 |
op_doi |
https://doi.org/10.1186/s12936-023-04485-7 |
container_title |
Malaria Journal |
container_volume |
22 |
container_issue |
1 |
_version_ |
1766348560117268480 |