A Novel Orthoreovirus Isolated From Dead Stranded Harbor Seals From Puget Sound, Washington State, United States
As part of an ongoing investigation of harbor seal (Phoca vitulina) mortalities within Puget Sound, Washington State, United States, between October 2007 and July 2008, 25 seal cases were submitted for histopathology and ancillary diagnostic testing, including additional attempted virus isolation. I...
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ftdoajarticles:oai:doaj.org/article:03cdded72805495085ffe3d912f60726 2023-05-15T16:33:09+02:00 A Novel Orthoreovirus Isolated From Dead Stranded Harbor Seals From Puget Sound, Washington State, United States Ole Nielsen Thaís C. S. Rodrigues Vsevolod L. Popov Kuttichantran Subramaniam Thomas B. Waltzek Dyanna M. Lambourn Stephen Raverty 2021-07-01T00:00:00Z https://doi.org/10.3389/fmars.2021.676725 https://doaj.org/article/03cdded72805495085ffe3d912f60726 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmars.2021.676725/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2021.676725 https://doaj.org/article/03cdded72805495085ffe3d912f60726 Frontiers in Marine Science, Vol 8 (2021) genome sequencing marine mammal mortality event investigation pinniped Reoviridae transmission electron microscopy Science Q General. Including nature conservation geographical distribution QH1-199.5 article 2021 ftdoajarticles https://doi.org/10.3389/fmars.2021.676725 2022-12-31T10:22:37Z As part of an ongoing investigation of harbor seal (Phoca vitulina) mortalities within Puget Sound, Washington State, United States, between October 2007 and July 2008, 25 seal cases were submitted for histopathology and ancillary diagnostic testing, including additional attempted virus isolation. In vitro granular and refractile cytopathic effects (CPE) were consistently observed in Vero.DogSLAMtag cells inoculated with tissue homogenates from three seals. Transmission electron microscopy of infected Vero.DogSLAMtag cells revealed cytoplasmic clusters of icosahedral viral particles morphologically consistent with members of the family Reoviridae. The complete genome of a novel species within the genus Orthoreovirus, tentatively named phocid orthoreovirus 1 (PhRV1), was determined by next-generation sequencing and confirmed by rt-PCR in isolates from the three harbor seals. This is the first report of an orthoreovirus infection associated with dead stranded harbor seals. Aside from the CPE and ultrastructural findings, no consistent signalment, gross pathology, histopathology, or ancillary diagnostic findings were identified with PhRV1 infection. Further research is needed to determine the prevalence, tissue tropism, transmission, pathogenicity, zoonotic potential, and host range of orthoreoviruses in pinnipeds. This study demonstrates the value of thorough necropsy investigations and a multidisciplinary team approach to advance our understanding of marine mammal health. Article in Journal/Newspaper harbor seal Phoca vitulina Directory of Open Access Journals: DOAJ Articles Frontiers in Marine Science 8 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
genome sequencing marine mammal mortality event investigation pinniped Reoviridae transmission electron microscopy Science Q General. Including nature conservation geographical distribution QH1-199.5 |
spellingShingle |
genome sequencing marine mammal mortality event investigation pinniped Reoviridae transmission electron microscopy Science Q General. Including nature conservation geographical distribution QH1-199.5 Ole Nielsen Thaís C. S. Rodrigues Vsevolod L. Popov Kuttichantran Subramaniam Thomas B. Waltzek Dyanna M. Lambourn Stephen Raverty A Novel Orthoreovirus Isolated From Dead Stranded Harbor Seals From Puget Sound, Washington State, United States |
topic_facet |
genome sequencing marine mammal mortality event investigation pinniped Reoviridae transmission electron microscopy Science Q General. Including nature conservation geographical distribution QH1-199.5 |
description |
As part of an ongoing investigation of harbor seal (Phoca vitulina) mortalities within Puget Sound, Washington State, United States, between October 2007 and July 2008, 25 seal cases were submitted for histopathology and ancillary diagnostic testing, including additional attempted virus isolation. In vitro granular and refractile cytopathic effects (CPE) were consistently observed in Vero.DogSLAMtag cells inoculated with tissue homogenates from three seals. Transmission electron microscopy of infected Vero.DogSLAMtag cells revealed cytoplasmic clusters of icosahedral viral particles morphologically consistent with members of the family Reoviridae. The complete genome of a novel species within the genus Orthoreovirus, tentatively named phocid orthoreovirus 1 (PhRV1), was determined by next-generation sequencing and confirmed by rt-PCR in isolates from the three harbor seals. This is the first report of an orthoreovirus infection associated with dead stranded harbor seals. Aside from the CPE and ultrastructural findings, no consistent signalment, gross pathology, histopathology, or ancillary diagnostic findings were identified with PhRV1 infection. Further research is needed to determine the prevalence, tissue tropism, transmission, pathogenicity, zoonotic potential, and host range of orthoreoviruses in pinnipeds. This study demonstrates the value of thorough necropsy investigations and a multidisciplinary team approach to advance our understanding of marine mammal health. |
format |
Article in Journal/Newspaper |
author |
Ole Nielsen Thaís C. S. Rodrigues Vsevolod L. Popov Kuttichantran Subramaniam Thomas B. Waltzek Dyanna M. Lambourn Stephen Raverty |
author_facet |
Ole Nielsen Thaís C. S. Rodrigues Vsevolod L. Popov Kuttichantran Subramaniam Thomas B. Waltzek Dyanna M. Lambourn Stephen Raverty |
author_sort |
Ole Nielsen |
title |
A Novel Orthoreovirus Isolated From Dead Stranded Harbor Seals From Puget Sound, Washington State, United States |
title_short |
A Novel Orthoreovirus Isolated From Dead Stranded Harbor Seals From Puget Sound, Washington State, United States |
title_full |
A Novel Orthoreovirus Isolated From Dead Stranded Harbor Seals From Puget Sound, Washington State, United States |
title_fullStr |
A Novel Orthoreovirus Isolated From Dead Stranded Harbor Seals From Puget Sound, Washington State, United States |
title_full_unstemmed |
A Novel Orthoreovirus Isolated From Dead Stranded Harbor Seals From Puget Sound, Washington State, United States |
title_sort |
novel orthoreovirus isolated from dead stranded harbor seals from puget sound, washington state, united states |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
https://doi.org/10.3389/fmars.2021.676725 https://doaj.org/article/03cdded72805495085ffe3d912f60726 |
genre |
harbor seal Phoca vitulina |
genre_facet |
harbor seal Phoca vitulina |
op_source |
Frontiers in Marine Science, Vol 8 (2021) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmars.2021.676725/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2021.676725 https://doaj.org/article/03cdded72805495085ffe3d912f60726 |
op_doi |
https://doi.org/10.3389/fmars.2021.676725 |
container_title |
Frontiers in Marine Science |
container_volume |
8 |
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1766022855702609920 |