Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis
Chromoblastomycosis (CBM) is a chronic subcutaneous infection caused by several dematiaceous fungi. The most commonly etiological agent found in Brazil is Fonsecaea pedrosoi, which appears as thick walled, brownish colored cells with transverse and longitudinal division in the lesions, called "...
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ftdoajarticles:oai:doaj.org/article:03c26dbc35774630968461e635c7fa37 2024-09-09T19:26:06+00:00 Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis Vidal Mônica Scarpelli Martinelli Castro Luis Guilherme Martins de Cavalecate Sônia Cristina Lacaz Carlos da Silva 2003-01-01T00:00:00Z https://doaj.org/article/03c26dbc35774630968461e635c7fa37 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652003000600003 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 0036-4665 1678-9946 https://doaj.org/article/03c26dbc35774630968461e635c7fa37 Revista do Instituto de Medicina Tropical de São Paulo, Vol 45, Iss 6, Pp 315-318 (2003) Chromoblastomycosis Fonsecaea pedrosoi Serologic tests and antigen Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2003 ftdoajarticles 2024-08-05T17:49:30Z Chromoblastomycosis (CBM) is a chronic subcutaneous infection caused by several dematiaceous fungi. The most commonly etiological agent found in Brazil is Fonsecaea pedrosoi, which appears as thick walled, brownish colored cells with transverse and longitudinal division in the lesions, called "muriform cells". This disease is found worldwide but countries like Madagascar and Brazil have highest incidence. Diagnosis is made by clinical, direct and histopathologic examination and culture of specimens. Serological tests have been used to identify specific antibodies against Fonsecaea pedrosoi antigens, as well as immunotechniques have been used for CBM serological identification and diagnosis. In the present study double immunodiffusion (DID), counterimmunoelectrophoresis (CIE) and immunoenzymatic test (ELISA) have been used to evaluate humoral immune response in patients with CBM caused by F. pedrosoi. Metabolic antigen was used for immunoprecipitation tests (DID and CIE) while somatic antigen for ELISA. Our results demonstrated 53% sensitivity and 96% specificity for DID, while CIE presented 68% sensitivity and 90.5% specificity. ELISA demonstrated 78% sensibility and 83% specificity. Serological tests can be a useful tool to study different aspects of CBM, such as helping differential diagnosis, when culture of the pathogenic agent is impossible. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Chromoblastomycosis Fonsecaea pedrosoi Serologic tests and antigen Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Chromoblastomycosis Fonsecaea pedrosoi Serologic tests and antigen Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Vidal Mônica Scarpelli Martinelli Castro Luis Guilherme Martins de Cavalecate Sônia Cristina Lacaz Carlos da Silva Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis |
topic_facet |
Chromoblastomycosis Fonsecaea pedrosoi Serologic tests and antigen Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Chromoblastomycosis (CBM) is a chronic subcutaneous infection caused by several dematiaceous fungi. The most commonly etiological agent found in Brazil is Fonsecaea pedrosoi, which appears as thick walled, brownish colored cells with transverse and longitudinal division in the lesions, called "muriform cells". This disease is found worldwide but countries like Madagascar and Brazil have highest incidence. Diagnosis is made by clinical, direct and histopathologic examination and culture of specimens. Serological tests have been used to identify specific antibodies against Fonsecaea pedrosoi antigens, as well as immunotechniques have been used for CBM serological identification and diagnosis. In the present study double immunodiffusion (DID), counterimmunoelectrophoresis (CIE) and immunoenzymatic test (ELISA) have been used to evaluate humoral immune response in patients with CBM caused by F. pedrosoi. Metabolic antigen was used for immunoprecipitation tests (DID and CIE) while somatic antigen for ELISA. Our results demonstrated 53% sensitivity and 96% specificity for DID, while CIE presented 68% sensitivity and 90.5% specificity. ELISA demonstrated 78% sensibility and 83% specificity. Serological tests can be a useful tool to study different aspects of CBM, such as helping differential diagnosis, when culture of the pathogenic agent is impossible. |
format |
Article in Journal/Newspaper |
author |
Vidal Mônica Scarpelli Martinelli Castro Luis Guilherme Martins de Cavalecate Sônia Cristina Lacaz Carlos da Silva |
author_facet |
Vidal Mônica Scarpelli Martinelli Castro Luis Guilherme Martins de Cavalecate Sônia Cristina Lacaz Carlos da Silva |
author_sort |
Vidal Mônica Scarpelli Martinelli |
title |
Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis |
title_short |
Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis |
title_full |
Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis |
title_fullStr |
Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis |
title_full_unstemmed |
Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis |
title_sort |
immunoprecipitation techniques and elisa in the detection of anti-fonsecaea pedrosoi antibodies in chromoblastomycosis |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2003 |
url |
https://doaj.org/article/03c26dbc35774630968461e635c7fa37 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 45, Iss 6, Pp 315-318 (2003) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652003000600003 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 0036-4665 1678-9946 https://doaj.org/article/03c26dbc35774630968461e635c7fa37 |
_version_ |
1809895774998233088 |