Histology and radiography studies of effects of Lepidium sativum seeds on bone healing in male albino rats
Lepidium sativum (garden cress) is a folk medicine that exhibited several therapeutic applications including anti-asthma, antihypertension, and bone healing. This study aimed to investigate the effects of L. sativum seeds on healing of fractured bone in male albino rats (Rattus rattus albinus). Twen...
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ftdoajarticles:oai:doaj.org/article:03721ae95e2a45efba1a9f9101f22a65 2023-05-15T18:05:41+02:00 Histology and radiography studies of effects of Lepidium sativum seeds on bone healing in male albino rats Bakeel A. Radman Bushra Y.H. Al-Khatib ZabnAllah M. Alaizeri AbdulHaleem S. Al-Tamimi Waleed E. Al-Thahibi Raja Mohan Hisham A. Alhadlaq Maqusood Ahamed 2022-07-01T00:00:00Z https://doi.org/10.1016/j.jksus.2022.102062 https://doaj.org/article/03721ae95e2a45efba1a9f9101f22a65 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S1018364722002439 https://doaj.org/toc/1018-3647 1018-3647 doi:10.1016/j.jksus.2022.102062 https://doaj.org/article/03721ae95e2a45efba1a9f9101f22a65 Journal of King Saud University: Science, Vol 34, Iss 5, Pp 102062- (2022) Lepidium sativum seeds Bone healing ALP Serum calcium Histology Radiography Science (General) Q1-390 article 2022 ftdoajarticles https://doi.org/10.1016/j.jksus.2022.102062 2022-12-31T02:40:09Z Lepidium sativum (garden cress) is a folk medicine that exhibited several therapeutic applications including anti-asthma, antihypertension, and bone healing. This study aimed to investigate the effects of L. sativum seeds on healing of fractured bone in male albino rats (Rattus rattus albinus). Twenty male albino rats were randomly divided into four groups (each group contains 5 rats); Non-Broken Control (NBC), Broken Control Non-Treated (BCNT), Broken Treated with 1 g/kg of seed powder (BT1g), and Broken Treated with 5 g/kg of seeds powder (BT5g). Right tibial closed fractures were created in the rats using a three-point bending technique. Serum Ca2+ level and Alkaline phosphatase tests were examined in all groups. Digital radiography was applied for three times, at the beginning, after 3 weeks, and 6 weeks. Histopathological study was done for right limb tibia bones. Results showed that the highest level of ALP was in the BT5g group after 3 weeks. However, after 6 weeks ALP level was the lowest in the BT5g group followed by the BT1g group. Serum Ca2+ level was higher in BT5g group after 6 weeks as compared to same group after 3 weeks. The X-ray photography shows that in treatment groups a clear improvement in bone healing that was revealed by an obvious callus and to a large extent a blurred fracture line. We also observed that after 6 weeks, the healing and recovery of broken bones were almost completed in L. sativum treated groups, especially in the BT5g group. Histology data further confirm the distinctive features of bone healing after feeding of seeds powder. Overall, our data suggested that seed of L. sativum has potential to speed up the callus formation, fracture line repairing, and new bone regeneration. This study highlights the importance of folk herbs in healing of fractured bones. Article in Journal/Newspaper Rattus rattus Directory of Open Access Journals: DOAJ Articles Journal of King Saud University - Science 34 5 102062 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Lepidium sativum seeds Bone healing ALP Serum calcium Histology Radiography Science (General) Q1-390 |
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Lepidium sativum seeds Bone healing ALP Serum calcium Histology Radiography Science (General) Q1-390 Bakeel A. Radman Bushra Y.H. Al-Khatib ZabnAllah M. Alaizeri AbdulHaleem S. Al-Tamimi Waleed E. Al-Thahibi Raja Mohan Hisham A. Alhadlaq Maqusood Ahamed Histology and radiography studies of effects of Lepidium sativum seeds on bone healing in male albino rats |
topic_facet |
Lepidium sativum seeds Bone healing ALP Serum calcium Histology Radiography Science (General) Q1-390 |
description |
Lepidium sativum (garden cress) is a folk medicine that exhibited several therapeutic applications including anti-asthma, antihypertension, and bone healing. This study aimed to investigate the effects of L. sativum seeds on healing of fractured bone in male albino rats (Rattus rattus albinus). Twenty male albino rats were randomly divided into four groups (each group contains 5 rats); Non-Broken Control (NBC), Broken Control Non-Treated (BCNT), Broken Treated with 1 g/kg of seed powder (BT1g), and Broken Treated with 5 g/kg of seeds powder (BT5g). Right tibial closed fractures were created in the rats using a three-point bending technique. Serum Ca2+ level and Alkaline phosphatase tests were examined in all groups. Digital radiography was applied for three times, at the beginning, after 3 weeks, and 6 weeks. Histopathological study was done for right limb tibia bones. Results showed that the highest level of ALP was in the BT5g group after 3 weeks. However, after 6 weeks ALP level was the lowest in the BT5g group followed by the BT1g group. Serum Ca2+ level was higher in BT5g group after 6 weeks as compared to same group after 3 weeks. The X-ray photography shows that in treatment groups a clear improvement in bone healing that was revealed by an obvious callus and to a large extent a blurred fracture line. We also observed that after 6 weeks, the healing and recovery of broken bones were almost completed in L. sativum treated groups, especially in the BT5g group. Histology data further confirm the distinctive features of bone healing after feeding of seeds powder. Overall, our data suggested that seed of L. sativum has potential to speed up the callus formation, fracture line repairing, and new bone regeneration. This study highlights the importance of folk herbs in healing of fractured bones. |
format |
Article in Journal/Newspaper |
author |
Bakeel A. Radman Bushra Y.H. Al-Khatib ZabnAllah M. Alaizeri AbdulHaleem S. Al-Tamimi Waleed E. Al-Thahibi Raja Mohan Hisham A. Alhadlaq Maqusood Ahamed |
author_facet |
Bakeel A. Radman Bushra Y.H. Al-Khatib ZabnAllah M. Alaizeri AbdulHaleem S. Al-Tamimi Waleed E. Al-Thahibi Raja Mohan Hisham A. Alhadlaq Maqusood Ahamed |
author_sort |
Bakeel A. Radman |
title |
Histology and radiography studies of effects of Lepidium sativum seeds on bone healing in male albino rats |
title_short |
Histology and radiography studies of effects of Lepidium sativum seeds on bone healing in male albino rats |
title_full |
Histology and radiography studies of effects of Lepidium sativum seeds on bone healing in male albino rats |
title_fullStr |
Histology and radiography studies of effects of Lepidium sativum seeds on bone healing in male albino rats |
title_full_unstemmed |
Histology and radiography studies of effects of Lepidium sativum seeds on bone healing in male albino rats |
title_sort |
histology and radiography studies of effects of lepidium sativum seeds on bone healing in male albino rats |
publisher |
Elsevier |
publishDate |
2022 |
url |
https://doi.org/10.1016/j.jksus.2022.102062 https://doaj.org/article/03721ae95e2a45efba1a9f9101f22a65 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Journal of King Saud University: Science, Vol 34, Iss 5, Pp 102062- (2022) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S1018364722002439 https://doaj.org/toc/1018-3647 1018-3647 doi:10.1016/j.jksus.2022.102062 https://doaj.org/article/03721ae95e2a45efba1a9f9101f22a65 |
op_doi |
https://doi.org/10.1016/j.jksus.2022.102062 |
container_title |
Journal of King Saud University - Science |
container_volume |
34 |
container_issue |
5 |
container_start_page |
102062 |
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1766177185014480896 |