Metabolite profiling and antidiabetic attributes of ultrasonicated leaf extracts of Conocarpus lancifolius
Objective: To profile the secondary metabolites and to evaluate the antidiabetic potential of hydroethanolic leaf extracts of Conocarpus lancifolius. Methods: The various hydroethanolic extracts of Conocarpus lancifolius leaf were prepared by ultrasonication assisted freeze- drying. Total phenolic c...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2020
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ftdoajarticles:oai:doaj.org/article:0365c04934f44611b9274b413f40032f 2023-05-15T15:13:55+02:00 Metabolite profiling and antidiabetic attributes of ultrasonicated leaf extracts of Conocarpus lancifolius Syed Ali Raza Ayoub Rashid Chaudhary Muhammad Waseem Mumtaz Ahmad Adnan Hamid Mukhtar Muhammad Tayyab Akhtar 2020-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.284430 https://doaj.org/article/0365c04934f44611b9274b413f40032f EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=8;spage=353;epage=360;aulast=Raza https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.284430 https://doaj.org/article/0365c04934f44611b9274b413f40032f Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 8, Pp 353-360 (2020) antioxidant antidiabetic conocarpus lancifolius metabolite profiling diabetic mice model Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.4103/2221-1691.284430 2022-12-31T12:12:01Z Objective: To profile the secondary metabolites and to evaluate the antidiabetic potential of hydroethanolic leaf extracts of Conocarpus lancifolius. Methods: The various hydroethanolic extracts of Conocarpus lancifolius leaf were prepared by ultrasonication assisted freeze- drying. Total phenolic contents, flavonoid contents, antioxidant activity, α-glucosidase and α-amylase inhibitions of leaf extracts were determined. The metabolite profiling was accomplished by UHPLC-Q-TOF-MS/MS analysis. The antidiabetic assessment of the most potent extract was carried out by measuring the hypoglycemic and hypolipidemic effect in the high fat diet-fed diabetic albino mice. The blood glucose level, haemoglobin, total cholesterol, high-density lipoproteins (HDL) and low-density lipoproteins (LDL) were determined. Results: The 60% ethanolic extract exhibited the highest phenolic and flavonoid contents of (349.39 ± 2.13) mg GAE/g dry extract and (116.95 ± 2.34) mg RE/g dry extracts, respectively, and the highest DPPH scavenging activity with an IC50 value of (32.87 ± 1.11) μg/ mL. The IC50 values for α-glucosidase and α-amylase inhibitions were (38.64 ± 0.93) μg/mL and (44.80 ± 1.57) μg/mL, respectively. UHPLC-Q-TOF-MS/MS analysis confirmed the presence of gallic acid, ellagic acid, corilagin, kaempherol-3-O-rutinoside, caffeic acid derivative, isorhamnetin and galloyl derivatives in the 60% ethanolic extract. Plant extract at a dose of 450 mg/kg body weight reduced blood glucose level, total cholesterol, LDL and HDL, and increased haemoglobin in alloxan-induced diabetic mice, Conclusions: Conocarpus lancifolius leaves are proved as a good source of biologically functional metabolites and possess antidiabetic activity which may be further explored to treat diabetes. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 10 8 353 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
antioxidant antidiabetic conocarpus lancifolius metabolite profiling diabetic mice model Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
antioxidant antidiabetic conocarpus lancifolius metabolite profiling diabetic mice model Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Syed Ali Raza Ayoub Rashid Chaudhary Muhammad Waseem Mumtaz Ahmad Adnan Hamid Mukhtar Muhammad Tayyab Akhtar Metabolite profiling and antidiabetic attributes of ultrasonicated leaf extracts of Conocarpus lancifolius |
topic_facet |
antioxidant antidiabetic conocarpus lancifolius metabolite profiling diabetic mice model Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To profile the secondary metabolites and to evaluate the antidiabetic potential of hydroethanolic leaf extracts of Conocarpus lancifolius. Methods: The various hydroethanolic extracts of Conocarpus lancifolius leaf were prepared by ultrasonication assisted freeze- drying. Total phenolic contents, flavonoid contents, antioxidant activity, α-glucosidase and α-amylase inhibitions of leaf extracts were determined. The metabolite profiling was accomplished by UHPLC-Q-TOF-MS/MS analysis. The antidiabetic assessment of the most potent extract was carried out by measuring the hypoglycemic and hypolipidemic effect in the high fat diet-fed diabetic albino mice. The blood glucose level, haemoglobin, total cholesterol, high-density lipoproteins (HDL) and low-density lipoproteins (LDL) were determined. Results: The 60% ethanolic extract exhibited the highest phenolic and flavonoid contents of (349.39 ± 2.13) mg GAE/g dry extract and (116.95 ± 2.34) mg RE/g dry extracts, respectively, and the highest DPPH scavenging activity with an IC50 value of (32.87 ± 1.11) μg/ mL. The IC50 values for α-glucosidase and α-amylase inhibitions were (38.64 ± 0.93) μg/mL and (44.80 ± 1.57) μg/mL, respectively. UHPLC-Q-TOF-MS/MS analysis confirmed the presence of gallic acid, ellagic acid, corilagin, kaempherol-3-O-rutinoside, caffeic acid derivative, isorhamnetin and galloyl derivatives in the 60% ethanolic extract. Plant extract at a dose of 450 mg/kg body weight reduced blood glucose level, total cholesterol, LDL and HDL, and increased haemoglobin in alloxan-induced diabetic mice, Conclusions: Conocarpus lancifolius leaves are proved as a good source of biologically functional metabolites and possess antidiabetic activity which may be further explored to treat diabetes. |
format |
Article in Journal/Newspaper |
author |
Syed Ali Raza Ayoub Rashid Chaudhary Muhammad Waseem Mumtaz Ahmad Adnan Hamid Mukhtar Muhammad Tayyab Akhtar |
author_facet |
Syed Ali Raza Ayoub Rashid Chaudhary Muhammad Waseem Mumtaz Ahmad Adnan Hamid Mukhtar Muhammad Tayyab Akhtar |
author_sort |
Syed Ali Raza |
title |
Metabolite profiling and antidiabetic attributes of ultrasonicated leaf extracts of Conocarpus lancifolius |
title_short |
Metabolite profiling and antidiabetic attributes of ultrasonicated leaf extracts of Conocarpus lancifolius |
title_full |
Metabolite profiling and antidiabetic attributes of ultrasonicated leaf extracts of Conocarpus lancifolius |
title_fullStr |
Metabolite profiling and antidiabetic attributes of ultrasonicated leaf extracts of Conocarpus lancifolius |
title_full_unstemmed |
Metabolite profiling and antidiabetic attributes of ultrasonicated leaf extracts of Conocarpus lancifolius |
title_sort |
metabolite profiling and antidiabetic attributes of ultrasonicated leaf extracts of conocarpus lancifolius |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2020 |
url |
https://doi.org/10.4103/2221-1691.284430 https://doaj.org/article/0365c04934f44611b9274b413f40032f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 8, Pp 353-360 (2020) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=8;spage=353;epage=360;aulast=Raza https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.284430 https://doaj.org/article/0365c04934f44611b9274b413f40032f |
op_doi |
https://doi.org/10.4103/2221-1691.284430 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
10 |
container_issue |
8 |
container_start_page |
353 |
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1766344430758920192 |