Pseudomonas fluorescens filamentous hemagglutinin, an iron-regulated protein, is an important virulence factor that modulates bacterial pathogenicity
Pseudomonas fluorescens is a common bacterial pathogen to a wide range of aquaculture animals including various species of fish. In this study, we employed proteomic analysis and identified filamentous hemagglutinin (FHA) as an iron-responsive protein secreted by TSS, a pathogenic P. fluorescens iso...
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ftdoajarticles:oai:doaj.org/article:03625f43d3d141d6b3ced09e96caf0a7 2023-05-15T18:41:14+02:00 Pseudomonas fluorescens filamentous hemagglutinin, an iron-regulated protein, is an important virulence factor that modulates bacterial pathogenicity Yuan-yuan Sun Heng Chi Li Sun 2016-08-01T00:00:00Z https://doi.org/10.3389/fmicb.2016.01320 https://doaj.org/article/03625f43d3d141d6b3ced09e96caf0a7 EN eng Frontiers Media S.A. http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01320/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2016.01320 https://doaj.org/article/03625f43d3d141d6b3ced09e96caf0a7 Frontiers in Microbiology, Vol 7 (2016) Pseudomonas fluorescens Virulence Vaccine Adhesion motility Filamentous hemagglutinin Microbiology QR1-502 article 2016 ftdoajarticles https://doi.org/10.3389/fmicb.2016.01320 2022-12-31T09:57:30Z Pseudomonas fluorescens is a common bacterial pathogen to a wide range of aquaculture animals including various species of fish. In this study, we employed proteomic analysis and identified filamentous hemagglutinin (FHA) as an iron-responsive protein secreted by TSS, a pathogenic P. fluorescens isolate. In vitro study showed that compared to the wild type, the fha mutant TSSfha (i) exhibited a largely similar vegetative growth profile but significantly retarded in the ability of biofilm growth and producing extracellular matrix, (ii) displayed no apparent flagella and motility, (iii) was defective in the attachment to host cells and unable to form self-aggregation, (iv) displayed markedly reduced capacity of hemagglutination and surviving in host serum. In vivo infection analysis revealed that TSSfha was significantly attenuated in the ability of dissemination in fish tissues and inducing host mortality, and that antibody blocking of the natural FHA produced by the wild type TSS impaired the infectivity of the pathogen. Furthermore, when introduced into turbot as a subunit vaccine, recombinant FHA elicited a significant protection against lethal TSS challenge. Taken together, these results indicate for the first time that P. fluorescens FHA is a key virulence factor essential to multiple biological processes associated with pathogenicity. Article in Journal/Newspaper Turbot Directory of Open Access Journals: DOAJ Articles Frontiers in Microbiology 7 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Pseudomonas fluorescens Virulence Vaccine Adhesion motility Filamentous hemagglutinin Microbiology QR1-502 |
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Pseudomonas fluorescens Virulence Vaccine Adhesion motility Filamentous hemagglutinin Microbiology QR1-502 Yuan-yuan Sun Heng Chi Li Sun Pseudomonas fluorescens filamentous hemagglutinin, an iron-regulated protein, is an important virulence factor that modulates bacterial pathogenicity |
topic_facet |
Pseudomonas fluorescens Virulence Vaccine Adhesion motility Filamentous hemagglutinin Microbiology QR1-502 |
description |
Pseudomonas fluorescens is a common bacterial pathogen to a wide range of aquaculture animals including various species of fish. In this study, we employed proteomic analysis and identified filamentous hemagglutinin (FHA) as an iron-responsive protein secreted by TSS, a pathogenic P. fluorescens isolate. In vitro study showed that compared to the wild type, the fha mutant TSSfha (i) exhibited a largely similar vegetative growth profile but significantly retarded in the ability of biofilm growth and producing extracellular matrix, (ii) displayed no apparent flagella and motility, (iii) was defective in the attachment to host cells and unable to form self-aggregation, (iv) displayed markedly reduced capacity of hemagglutination and surviving in host serum. In vivo infection analysis revealed that TSSfha was significantly attenuated in the ability of dissemination in fish tissues and inducing host mortality, and that antibody blocking of the natural FHA produced by the wild type TSS impaired the infectivity of the pathogen. Furthermore, when introduced into turbot as a subunit vaccine, recombinant FHA elicited a significant protection against lethal TSS challenge. Taken together, these results indicate for the first time that P. fluorescens FHA is a key virulence factor essential to multiple biological processes associated with pathogenicity. |
format |
Article in Journal/Newspaper |
author |
Yuan-yuan Sun Heng Chi Li Sun |
author_facet |
Yuan-yuan Sun Heng Chi Li Sun |
author_sort |
Yuan-yuan Sun |
title |
Pseudomonas fluorescens filamentous hemagglutinin, an iron-regulated protein, is an important virulence factor that modulates bacterial pathogenicity |
title_short |
Pseudomonas fluorescens filamentous hemagglutinin, an iron-regulated protein, is an important virulence factor that modulates bacterial pathogenicity |
title_full |
Pseudomonas fluorescens filamentous hemagglutinin, an iron-regulated protein, is an important virulence factor that modulates bacterial pathogenicity |
title_fullStr |
Pseudomonas fluorescens filamentous hemagglutinin, an iron-regulated protein, is an important virulence factor that modulates bacterial pathogenicity |
title_full_unstemmed |
Pseudomonas fluorescens filamentous hemagglutinin, an iron-regulated protein, is an important virulence factor that modulates bacterial pathogenicity |
title_sort |
pseudomonas fluorescens filamentous hemagglutinin, an iron-regulated protein, is an important virulence factor that modulates bacterial pathogenicity |
publisher |
Frontiers Media S.A. |
publishDate |
2016 |
url |
https://doi.org/10.3389/fmicb.2016.01320 https://doaj.org/article/03625f43d3d141d6b3ced09e96caf0a7 |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
Frontiers in Microbiology, Vol 7 (2016) |
op_relation |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01320/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2016.01320 https://doaj.org/article/03625f43d3d141d6b3ced09e96caf0a7 |
op_doi |
https://doi.org/10.3389/fmicb.2016.01320 |
container_title |
Frontiers in Microbiology |
container_volume |
7 |
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1766230733363347456 |