Technical Note: A minimally invasive experimental system for p CO 2 manipulation in plankton cultures using passive gas exchange (atmospheric carbon control simulator)
As research into the biotic effects of ocean acidification has increased, the methods for simulating these environmental changes in the laboratory have multiplied. Here we describe the atmospheric carbon control simulator (ACCS) for the maintenance of plankton under controlled p CO 2 conditions, des...
Published in: | Biogeosciences |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Copernicus Publications
2017
|
Subjects: | |
Online Access: | https://doi.org/10.5194/bg-14-2675-2017 https://doaj.org/article/035e833d150b4e58b670ce31d8de2dfb |
id |
ftdoajarticles:oai:doaj.org/article:035e833d150b4e58b670ce31d8de2dfb |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:035e833d150b4e58b670ce31d8de2dfb 2023-05-15T17:50:27+02:00 Technical Note: A minimally invasive experimental system for p CO 2 manipulation in plankton cultures using passive gas exchange (atmospheric carbon control simulator) B. A. Love M. B. Olson T. Wuori 2017-05-01T00:00:00Z https://doi.org/10.5194/bg-14-2675-2017 https://doaj.org/article/035e833d150b4e58b670ce31d8de2dfb EN eng Copernicus Publications http://www.biogeosciences.net/14/2675/2017/bg-14-2675-2017.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 1726-4170 1726-4189 doi:10.5194/bg-14-2675-2017 https://doaj.org/article/035e833d150b4e58b670ce31d8de2dfb Biogeosciences, Vol 14, Iss 10, Pp 2675-2684 (2017) Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 article 2017 ftdoajarticles https://doi.org/10.5194/bg-14-2675-2017 2022-12-31T04:19:42Z As research into the biotic effects of ocean acidification has increased, the methods for simulating these environmental changes in the laboratory have multiplied. Here we describe the atmospheric carbon control simulator (ACCS) for the maintenance of plankton under controlled p CO 2 conditions, designed for species sensitive to the physical disturbance introduced by the bubbling of cultures and for studies involving trophic interaction. The system consists of gas mixing and equilibration components coupled with large-volume atmospheric simulation chambers. These chambers allow gas exchange to counteract the changes in carbonate chemistry induced by the metabolic activity of the organisms. The system is relatively low cost, very flexible, and when used in conjunction with semi-continuous culture methods, it increases the density of organisms kept under realistic conditions, increases the allowable time interval between dilutions, and/or decreases the metabolically driven change in carbonate chemistry during these intervals. It accommodates a large number of culture vessels, which facilitate multi-trophic level studies and allow the tracking of variable responses within and across plankton populations to ocean acidification. It also includes components that increase the reliability of gas mixing systems using mass flow controllers. Article in Journal/Newspaper Ocean acidification Directory of Open Access Journals: DOAJ Articles Biogeosciences 14 10 2675 2684 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
spellingShingle |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 B. A. Love M. B. Olson T. Wuori Technical Note: A minimally invasive experimental system for p CO 2 manipulation in plankton cultures using passive gas exchange (atmospheric carbon control simulator) |
topic_facet |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
description |
As research into the biotic effects of ocean acidification has increased, the methods for simulating these environmental changes in the laboratory have multiplied. Here we describe the atmospheric carbon control simulator (ACCS) for the maintenance of plankton under controlled p CO 2 conditions, designed for species sensitive to the physical disturbance introduced by the bubbling of cultures and for studies involving trophic interaction. The system consists of gas mixing and equilibration components coupled with large-volume atmospheric simulation chambers. These chambers allow gas exchange to counteract the changes in carbonate chemistry induced by the metabolic activity of the organisms. The system is relatively low cost, very flexible, and when used in conjunction with semi-continuous culture methods, it increases the density of organisms kept under realistic conditions, increases the allowable time interval between dilutions, and/or decreases the metabolically driven change in carbonate chemistry during these intervals. It accommodates a large number of culture vessels, which facilitate multi-trophic level studies and allow the tracking of variable responses within and across plankton populations to ocean acidification. It also includes components that increase the reliability of gas mixing systems using mass flow controllers. |
format |
Article in Journal/Newspaper |
author |
B. A. Love M. B. Olson T. Wuori |
author_facet |
B. A. Love M. B. Olson T. Wuori |
author_sort |
B. A. Love |
title |
Technical Note: A minimally invasive experimental system for p CO 2 manipulation in plankton cultures using passive gas exchange (atmospheric carbon control simulator) |
title_short |
Technical Note: A minimally invasive experimental system for p CO 2 manipulation in plankton cultures using passive gas exchange (atmospheric carbon control simulator) |
title_full |
Technical Note: A minimally invasive experimental system for p CO 2 manipulation in plankton cultures using passive gas exchange (atmospheric carbon control simulator) |
title_fullStr |
Technical Note: A minimally invasive experimental system for p CO 2 manipulation in plankton cultures using passive gas exchange (atmospheric carbon control simulator) |
title_full_unstemmed |
Technical Note: A minimally invasive experimental system for p CO 2 manipulation in plankton cultures using passive gas exchange (atmospheric carbon control simulator) |
title_sort |
technical note: a minimally invasive experimental system for p co 2 manipulation in plankton cultures using passive gas exchange (atmospheric carbon control simulator) |
publisher |
Copernicus Publications |
publishDate |
2017 |
url |
https://doi.org/10.5194/bg-14-2675-2017 https://doaj.org/article/035e833d150b4e58b670ce31d8de2dfb |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Biogeosciences, Vol 14, Iss 10, Pp 2675-2684 (2017) |
op_relation |
http://www.biogeosciences.net/14/2675/2017/bg-14-2675-2017.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 1726-4170 1726-4189 doi:10.5194/bg-14-2675-2017 https://doaj.org/article/035e833d150b4e58b670ce31d8de2dfb |
op_doi |
https://doi.org/10.5194/bg-14-2675-2017 |
container_title |
Biogeosciences |
container_volume |
14 |
container_issue |
10 |
container_start_page |
2675 |
op_container_end_page |
2684 |
_version_ |
1766157216483639296 |