Bartonella spp. bacteremia in blood donors from Campinas, Brazil.
Bartonella species are blood-borne, re-emerging organisms, capable of causing prolonged infection with diverse disease manifestations, from asymptomatic bacteremia to chronic debilitating disease and death. This pathogen can survive for over a month in stored blood. However, its prevalence among blo...
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ftdoajarticles:oai:doaj.org/article:0349446ee0284c3c983b0ffbbf62b942 2023-05-15T15:12:19+02:00 Bartonella spp. bacteremia in blood donors from Campinas, Brazil. Luiza Helena Urso Pitassi Pedro Paulo Vissotto de Paiva Diniz Diana Gerardi Scorpio Marina Rovani Drummond Bruno Grosselli Lania Maria Lourdes Barjas-Castro Rovilson Gilioli Silvia Colombo Stanley Sowy Edward B Breitschwerdt William L Nicholson Paulo Eduardo Neves Ferreira Velho 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003467 https://doaj.org/article/0349446ee0284c3c983b0ffbbf62b942 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4295888?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003467 https://doaj.org/article/0349446ee0284c3c983b0ffbbf62b942 PLoS Neglected Tropical Diseases, Vol 9, Iss 1, p e0003467 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003467 2022-12-31T10:38:05Z Bartonella species are blood-borne, re-emerging organisms, capable of causing prolonged infection with diverse disease manifestations, from asymptomatic bacteremia to chronic debilitating disease and death. This pathogen can survive for over a month in stored blood. However, its prevalence among blood donors is unknown, and screening of blood supplies for this pathogen is not routinely performed. We investigated Bartonella spp. prevalence in 500 blood donors from Campinas, Brazil, based on a cross-sectional design. Blood samples were inoculated into an enrichment liquid growth medium and sub-inoculated onto blood agar. Liquid culture samples and Gram-negative isolates were tested using a genus specific ITS PCR with amplicons sequenced for species identification. Bartonella henselae and Bartonella quintana antibodies were assayed by indirect immunofluorescence. B. henselae was isolated from six donors (1.2%). Sixteen donors (3.2%) were Bartonella-PCR positive after culture in liquid or on solid media, with 15 donors infected with B. henselae and one donor infected with Bartonella clarridgeiae. Antibodies against B. henselae or B. quintana were found in 16% and 32% of 500 blood donors, respectively. Serology was not associated with infection, with only three of 16 Bartonella-infected subjects seropositive for B. henselae or B. quintana. Bartonella DNA was present in the bloodstream of approximately one out of 30 donors from a major blood bank in South America. Negative serology does not rule out Bartonella spp. infection in healthy subjects. Using a combination of liquid and solid cultures, PCR, and DNA sequencing, this study documents for the first time that Bartonella spp. bacteremia occurs in asymptomatic blood donors. Our findings support further evaluation of Bartonella spp. transmission which can occur through blood transfusions. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 1 e0003467 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Luiza Helena Urso Pitassi Pedro Paulo Vissotto de Paiva Diniz Diana Gerardi Scorpio Marina Rovani Drummond Bruno Grosselli Lania Maria Lourdes Barjas-Castro Rovilson Gilioli Silvia Colombo Stanley Sowy Edward B Breitschwerdt William L Nicholson Paulo Eduardo Neves Ferreira Velho Bartonella spp. bacteremia in blood donors from Campinas, Brazil. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Bartonella species are blood-borne, re-emerging organisms, capable of causing prolonged infection with diverse disease manifestations, from asymptomatic bacteremia to chronic debilitating disease and death. This pathogen can survive for over a month in stored blood. However, its prevalence among blood donors is unknown, and screening of blood supplies for this pathogen is not routinely performed. We investigated Bartonella spp. prevalence in 500 blood donors from Campinas, Brazil, based on a cross-sectional design. Blood samples were inoculated into an enrichment liquid growth medium and sub-inoculated onto blood agar. Liquid culture samples and Gram-negative isolates were tested using a genus specific ITS PCR with amplicons sequenced for species identification. Bartonella henselae and Bartonella quintana antibodies were assayed by indirect immunofluorescence. B. henselae was isolated from six donors (1.2%). Sixteen donors (3.2%) were Bartonella-PCR positive after culture in liquid or on solid media, with 15 donors infected with B. henselae and one donor infected with Bartonella clarridgeiae. Antibodies against B. henselae or B. quintana were found in 16% and 32% of 500 blood donors, respectively. Serology was not associated with infection, with only three of 16 Bartonella-infected subjects seropositive for B. henselae or B. quintana. Bartonella DNA was present in the bloodstream of approximately one out of 30 donors from a major blood bank in South America. Negative serology does not rule out Bartonella spp. infection in healthy subjects. Using a combination of liquid and solid cultures, PCR, and DNA sequencing, this study documents for the first time that Bartonella spp. bacteremia occurs in asymptomatic blood donors. Our findings support further evaluation of Bartonella spp. transmission which can occur through blood transfusions. |
format |
Article in Journal/Newspaper |
author |
Luiza Helena Urso Pitassi Pedro Paulo Vissotto de Paiva Diniz Diana Gerardi Scorpio Marina Rovani Drummond Bruno Grosselli Lania Maria Lourdes Barjas-Castro Rovilson Gilioli Silvia Colombo Stanley Sowy Edward B Breitschwerdt William L Nicholson Paulo Eduardo Neves Ferreira Velho |
author_facet |
Luiza Helena Urso Pitassi Pedro Paulo Vissotto de Paiva Diniz Diana Gerardi Scorpio Marina Rovani Drummond Bruno Grosselli Lania Maria Lourdes Barjas-Castro Rovilson Gilioli Silvia Colombo Stanley Sowy Edward B Breitschwerdt William L Nicholson Paulo Eduardo Neves Ferreira Velho |
author_sort |
Luiza Helena Urso Pitassi |
title |
Bartonella spp. bacteremia in blood donors from Campinas, Brazil. |
title_short |
Bartonella spp. bacteremia in blood donors from Campinas, Brazil. |
title_full |
Bartonella spp. bacteremia in blood donors from Campinas, Brazil. |
title_fullStr |
Bartonella spp. bacteremia in blood donors from Campinas, Brazil. |
title_full_unstemmed |
Bartonella spp. bacteremia in blood donors from Campinas, Brazil. |
title_sort |
bartonella spp. bacteremia in blood donors from campinas, brazil. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0003467 https://doaj.org/article/0349446ee0284c3c983b0ffbbf62b942 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 1, p e0003467 (2015) |
op_relation |
http://europepmc.org/articles/PMC4295888?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003467 https://doaj.org/article/0349446ee0284c3c983b0ffbbf62b942 |
op_doi |
https://doi.org/10.1371/journal.pntd.0003467 |
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PLOS Neglected Tropical Diseases |
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9 |
container_issue |
1 |
container_start_page |
e0003467 |
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1766343012301930496 |