Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum
Introduction Kala-azar is a disease resulting from infection by Leishmania donovani and Leishmania infantum. Most patients with the disease exhibit prolonged fever, wasting, anemia and hepatosplenomegaly without complications. However, some patients develop severe disease with hemorrhagic manifestat...
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ftdoajarticles:oai:doaj.org/article:0339e28f5f8f4f6f808376c65540e658 2023-05-15T15:13:09+02:00 Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum Bruno Guedes Alcoforado Aguiar Daniela Lemos Coelho Dorcas Lamounier Costa Betânia Paiva Drumond Luiz Felipe Leomil Coelho Lívio Carvalho Figueiredo Danielle Alves Zacarias Jailthon Carlos da Silva Diego Peres Alonso Paulo Eduardo Martins Ribolla Edna Aoba Yassui Ishikawa Samara Belchior Gaído Carlos Henrique Nery Costa 2014-10-01T00:00:00Z https://doi.org/10.1590/0037-8682-0183-2014 https://doaj.org/article/0339e28f5f8f4f6f808376c65540e658 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000500593&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0183-2014 https://doaj.org/article/0339e28f5f8f4f6f808376c65540e658 Revista da Sociedade Brasileira de Medicina Tropical, Vol 47, Iss 5, Pp 593-598 (2014) Genetic diversity Kala-azar Visceral leishmaniasis Macrophage inhibition factor Tropical diseases Single nucleotide polymorphism Arctic medicine. Tropical medicine RC955-962 article 2014 ftdoajarticles https://doi.org/10.1590/0037-8682-0183-2014 2022-12-30T21:39:01Z Introduction Kala-azar is a disease resulting from infection by Leishmania donovani and Leishmania infantum. Most patients with the disease exhibit prolonged fever, wasting, anemia and hepatosplenomegaly without complications. However, some patients develop severe disease with hemorrhagic manifestations, bacterial infections, jaundice, and edema dyspnea, among other symptoms, followed by death. Among the parasite molecules that might influence the disease severity are the macrophage migration inhibitory factor-like proteins (MIF1 and MIF2) and N-acetylglucosamine-1-phosphotransferase (NAGT), which act in the first step of protein N-glycosylation. This study aimed to determine whether MIF1, MIF2 and NAGT are virulence factors for severe kala-azar. Methods To determine the parasite genotype in kala-azar patients from Northeastern Brazil, we sequenced the NAGT genes of L. infantum from 68 patients as well as the MIF1 and MIF2 genes from 76 different subjects with diverse clinical manifestations. After polymerase chain reaction (PCR), the fragments were sequenced, followed by polymorphism identification. Results The nucleotide sequencing of the 144 amplicons revealed the absence of genetic variability of the NAGT, MIF1 and MIF2 genes between the isolates. The conservation of these genes suggests that the clinical variability of kala-azar does not depend upon these genes. Additionally, this conservation suggests that these genes may be critical for parasite survival. Conclusions NAGT, MIF1 and MIF2 do not alter the severity of kala-azar. NAGT, MIF1 and MIF2 are highly conserved among different isolates of identical species and exhibit potential for use in phylogenetic inferences or molecular diagnosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Azar ENVELOPE(-63.733,-63.733,-64.983,-64.983) Revista da Sociedade Brasileira de Medicina Tropical 47 5 593 598 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Genetic diversity Kala-azar Visceral leishmaniasis Macrophage inhibition factor Tropical diseases Single nucleotide polymorphism Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Genetic diversity Kala-azar Visceral leishmaniasis Macrophage inhibition factor Tropical diseases Single nucleotide polymorphism Arctic medicine. Tropical medicine RC955-962 Bruno Guedes Alcoforado Aguiar Daniela Lemos Coelho Dorcas Lamounier Costa Betânia Paiva Drumond Luiz Felipe Leomil Coelho Lívio Carvalho Figueiredo Danielle Alves Zacarias Jailthon Carlos da Silva Diego Peres Alonso Paulo Eduardo Martins Ribolla Edna Aoba Yassui Ishikawa Samara Belchior Gaído Carlos Henrique Nery Costa Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum |
topic_facet |
Genetic diversity Kala-azar Visceral leishmaniasis Macrophage inhibition factor Tropical diseases Single nucleotide polymorphism Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction Kala-azar is a disease resulting from infection by Leishmania donovani and Leishmania infantum. Most patients with the disease exhibit prolonged fever, wasting, anemia and hepatosplenomegaly without complications. However, some patients develop severe disease with hemorrhagic manifestations, bacterial infections, jaundice, and edema dyspnea, among other symptoms, followed by death. Among the parasite molecules that might influence the disease severity are the macrophage migration inhibitory factor-like proteins (MIF1 and MIF2) and N-acetylglucosamine-1-phosphotransferase (NAGT), which act in the first step of protein N-glycosylation. This study aimed to determine whether MIF1, MIF2 and NAGT are virulence factors for severe kala-azar. Methods To determine the parasite genotype in kala-azar patients from Northeastern Brazil, we sequenced the NAGT genes of L. infantum from 68 patients as well as the MIF1 and MIF2 genes from 76 different subjects with diverse clinical manifestations. After polymerase chain reaction (PCR), the fragments were sequenced, followed by polymorphism identification. Results The nucleotide sequencing of the 144 amplicons revealed the absence of genetic variability of the NAGT, MIF1 and MIF2 genes between the isolates. The conservation of these genes suggests that the clinical variability of kala-azar does not depend upon these genes. Additionally, this conservation suggests that these genes may be critical for parasite survival. Conclusions NAGT, MIF1 and MIF2 do not alter the severity of kala-azar. NAGT, MIF1 and MIF2 are highly conserved among different isolates of identical species and exhibit potential for use in phylogenetic inferences or molecular diagnosis. |
format |
Article in Journal/Newspaper |
author |
Bruno Guedes Alcoforado Aguiar Daniela Lemos Coelho Dorcas Lamounier Costa Betânia Paiva Drumond Luiz Felipe Leomil Coelho Lívio Carvalho Figueiredo Danielle Alves Zacarias Jailthon Carlos da Silva Diego Peres Alonso Paulo Eduardo Martins Ribolla Edna Aoba Yassui Ishikawa Samara Belchior Gaído Carlos Henrique Nery Costa |
author_facet |
Bruno Guedes Alcoforado Aguiar Daniela Lemos Coelho Dorcas Lamounier Costa Betânia Paiva Drumond Luiz Felipe Leomil Coelho Lívio Carvalho Figueiredo Danielle Alves Zacarias Jailthon Carlos da Silva Diego Peres Alonso Paulo Eduardo Martins Ribolla Edna Aoba Yassui Ishikawa Samara Belchior Gaído Carlos Henrique Nery Costa |
author_sort |
Bruno Guedes Alcoforado Aguiar |
title |
Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum |
title_short |
Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum |
title_full |
Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum |
title_fullStr |
Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum |
title_full_unstemmed |
Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum |
title_sort |
genes that encodes nagt, mif1 and mif2 are not virulence factors for kala-azar caused by leishmania infantum |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2014 |
url |
https://doi.org/10.1590/0037-8682-0183-2014 https://doaj.org/article/0339e28f5f8f4f6f808376c65540e658 |
long_lat |
ENVELOPE(-63.733,-63.733,-64.983,-64.983) |
geographic |
Arctic Azar |
geographic_facet |
Arctic Azar |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 47, Iss 5, Pp 593-598 (2014) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000500593&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0183-2014 https://doaj.org/article/0339e28f5f8f4f6f808376c65540e658 |
op_doi |
https://doi.org/10.1590/0037-8682-0183-2014 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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47 |
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