Expression of annexin-A1 in blood and tissue leukocytes of leprosy patients
Abstract INTRODUCTION In leprosy, immune system mediators that regulate the infectious process act in a complex manner and can lead to several clinical outcomes. To understand the behavior of these mediators we quantified the expression of annexin-A1 (ANXA1) in the peripheral blood and plasma as wel...
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ftdoajarticles:oai:doaj.org/article:03289d4fb25f45de90e41bfdc57f7cab 2023-05-15T15:11:04+02:00 Expression of annexin-A1 in blood and tissue leukocytes of leprosy patients Afonso Bezerra Ribeiro Caroline Marques Caloi Silvia Thais Sá Pimenta Sudha Seshayyan Srinivas Govindarajulu Francisco José Dutra Souto Amílcar Sabino Damazo 2020-11-01T00:00:00Z https://doi.org/10.1590/0037-8682-0277-2020 https://doaj.org/article/03289d4fb25f45de90e41bfdc57f7cab EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822020000100381&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0277-2020 https://doaj.org/article/03289d4fb25f45de90e41bfdc57f7cab Revista da Sociedade Brasileira de Medicina Tropical, Vol 53 (2020) Leprosy Annexin A1 Leukocytes Tuberculosis Lepromatous Arctic medicine. Tropical medicine RC955-962 article 2020 ftdoajarticles https://doi.org/10.1590/0037-8682-0277-2020 2022-12-30T23:24:25Z Abstract INTRODUCTION In leprosy, immune system mediators that regulate the infectious process act in a complex manner and can lead to several clinical outcomes. To understand the behavior of these mediators we quantified the expression of annexin-A1 (ANXA1) in the peripheral blood and plasma as well as tissue leukocytes in all clinical forms of leprosy and compared with healthy controls. METHODS Seventy healthy controls and 70 patients with leprosy, tuberculoid (TT) (n = 13), borderline tuberculoid (BT) (n = 15), borderline borderline (BB) (n = 13), borderline lepromatous (BL) (n = 15), and lepromatous leprosy (LL) (n = 14), were selected. Phenotyping of the lymphocyte cells and the intracellular expression of ANXA1 in leukocytes was performed by immunofluorescence. Plasma protein levels were determined by enzyme-linked immunosorbent assay. RESULTS Histiocytes and CD4+ and CD8+ T cells in the skin of BL and LL patients had higher ANXA1 expression. ANXA1 expression was also high in circulating polymorphonuclear, monocytes, and CD4+ and CD8+ T cells in the blood of LL patients compared to those of TT, BT, BB, and BL patients, and these levels were similar to those in healthy controls. Plasma ANXA1 levels indicate an increase in paracrine release in patients with LL. CONCLUSIONS The data indicate that ANXA1 expression is enhanced in the leukocytes and plasma of patients with LL, and may contribute to the inhibition of leukocyte action, leading to inadequate functioning of the immune system and thus contributing to the spread of M. leprae infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 53 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Leprosy Annexin A1 Leukocytes Tuberculosis Lepromatous Arctic medicine. Tropical medicine RC955-962 |
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Leprosy Annexin A1 Leukocytes Tuberculosis Lepromatous Arctic medicine. Tropical medicine RC955-962 Afonso Bezerra Ribeiro Caroline Marques Caloi Silvia Thais Sá Pimenta Sudha Seshayyan Srinivas Govindarajulu Francisco José Dutra Souto Amílcar Sabino Damazo Expression of annexin-A1 in blood and tissue leukocytes of leprosy patients |
topic_facet |
Leprosy Annexin A1 Leukocytes Tuberculosis Lepromatous Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract INTRODUCTION In leprosy, immune system mediators that regulate the infectious process act in a complex manner and can lead to several clinical outcomes. To understand the behavior of these mediators we quantified the expression of annexin-A1 (ANXA1) in the peripheral blood and plasma as well as tissue leukocytes in all clinical forms of leprosy and compared with healthy controls. METHODS Seventy healthy controls and 70 patients with leprosy, tuberculoid (TT) (n = 13), borderline tuberculoid (BT) (n = 15), borderline borderline (BB) (n = 13), borderline lepromatous (BL) (n = 15), and lepromatous leprosy (LL) (n = 14), were selected. Phenotyping of the lymphocyte cells and the intracellular expression of ANXA1 in leukocytes was performed by immunofluorescence. Plasma protein levels were determined by enzyme-linked immunosorbent assay. RESULTS Histiocytes and CD4+ and CD8+ T cells in the skin of BL and LL patients had higher ANXA1 expression. ANXA1 expression was also high in circulating polymorphonuclear, monocytes, and CD4+ and CD8+ T cells in the blood of LL patients compared to those of TT, BT, BB, and BL patients, and these levels were similar to those in healthy controls. Plasma ANXA1 levels indicate an increase in paracrine release in patients with LL. CONCLUSIONS The data indicate that ANXA1 expression is enhanced in the leukocytes and plasma of patients with LL, and may contribute to the inhibition of leukocyte action, leading to inadequate functioning of the immune system and thus contributing to the spread of M. leprae infection. |
format |
Article in Journal/Newspaper |
author |
Afonso Bezerra Ribeiro Caroline Marques Caloi Silvia Thais Sá Pimenta Sudha Seshayyan Srinivas Govindarajulu Francisco José Dutra Souto Amílcar Sabino Damazo |
author_facet |
Afonso Bezerra Ribeiro Caroline Marques Caloi Silvia Thais Sá Pimenta Sudha Seshayyan Srinivas Govindarajulu Francisco José Dutra Souto Amílcar Sabino Damazo |
author_sort |
Afonso Bezerra Ribeiro |
title |
Expression of annexin-A1 in blood and tissue leukocytes of leprosy patients |
title_short |
Expression of annexin-A1 in blood and tissue leukocytes of leprosy patients |
title_full |
Expression of annexin-A1 in blood and tissue leukocytes of leprosy patients |
title_fullStr |
Expression of annexin-A1 in blood and tissue leukocytes of leprosy patients |
title_full_unstemmed |
Expression of annexin-A1 in blood and tissue leukocytes of leprosy patients |
title_sort |
expression of annexin-a1 in blood and tissue leukocytes of leprosy patients |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2020 |
url |
https://doi.org/10.1590/0037-8682-0277-2020 https://doaj.org/article/03289d4fb25f45de90e41bfdc57f7cab |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 53 (2020) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822020000100381&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0277-2020 https://doaj.org/article/03289d4fb25f45de90e41bfdc57f7cab |
op_doi |
https://doi.org/10.1590/0037-8682-0277-2020 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
container_volume |
53 |
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1766341974171844608 |