Comparative genomic and phenotypic characterization of invasive non-typhoidal Salmonella isolates from Siaya, Kenya.
Non-typhoidal Salmonella (NTS) is a major global health concern that often causes bloodstream infections in areas of the world affected by malnutrition and comorbidities such as HIV and malaria. Developing a strategy to control the emergence and spread of highly invasive and antimicrobial resistant...
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ftdoajarticles:oai:doaj.org/article:0316fd1fe8794975a6b88fbd139a1722 2023-05-15T15:09:14+02:00 Comparative genomic and phenotypic characterization of invasive non-typhoidal Salmonella isolates from Siaya, Kenya. Jessica Z Kubicek-Sutherland Gary Xie Migun Shakya Priya K Dighe Lindsey L Jacobs Hajnalka Daligault Karen Davenport Loreen R Stromberg Zachary R Stromberg Qiuying Cheng Prakasha Kempaiah John Michael Ong'echa Vincent Otieno Evans Raballah Samuel Anyona Collins Ouma Patrick S G Chain Douglas J Perkins Harshini Mukundan Benjamin H McMahon Norman A Doggett 2021-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008991 https://doaj.org/article/0316fd1fe8794975a6b88fbd139a1722 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008991 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008991 https://doaj.org/article/0316fd1fe8794975a6b88fbd139a1722 PLoS Neglected Tropical Diseases, Vol 15, Iss 2, p e0008991 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008991 2022-12-31T11:49:06Z Non-typhoidal Salmonella (NTS) is a major global health concern that often causes bloodstream infections in areas of the world affected by malnutrition and comorbidities such as HIV and malaria. Developing a strategy to control the emergence and spread of highly invasive and antimicrobial resistant NTS isolates requires a comprehensive analysis of epidemiological factors and molecular pathogenesis. Here, we characterize 11 NTS isolates that caused bloodstream infections in pediatric patients in Siaya, Kenya from 2003-2010. Nine isolates were identified as S. Typhimurium sequence type 313 while the other two were S. Enteritidis. Comprehensive genotypic and phenotypic analyses were performed to compare these isolates to those previously identified in sub-Saharan Africa. We identified a S. Typhimurium isolate referred to as UGA14 that displayed novel plasmid, pseudogene and resistance features as compared to other isolates reported from Africa. Notably, UGA14 is able to ferment both lactose and sucrose due to the acquisition of insertion elements on the pKST313 plasmid. These findings show for the first time the co-evolution of plasmid-mediated lactose and sucrose metabolism along with cephalosporin resistance in NTS further elucidating the evolutionary mechanisms of invasive NTS phenotypes. These results further support the use of combined genomic and phenotypic approaches to detect and characterize atypical NTS isolates in order to advance biosurveillance efforts that inform countermeasures aimed at controlling invasive and antimicrobial resistant NTS. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 2 e0008991 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Jessica Z Kubicek-Sutherland Gary Xie Migun Shakya Priya K Dighe Lindsey L Jacobs Hajnalka Daligault Karen Davenport Loreen R Stromberg Zachary R Stromberg Qiuying Cheng Prakasha Kempaiah John Michael Ong'echa Vincent Otieno Evans Raballah Samuel Anyona Collins Ouma Patrick S G Chain Douglas J Perkins Harshini Mukundan Benjamin H McMahon Norman A Doggett Comparative genomic and phenotypic characterization of invasive non-typhoidal Salmonella isolates from Siaya, Kenya. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Non-typhoidal Salmonella (NTS) is a major global health concern that often causes bloodstream infections in areas of the world affected by malnutrition and comorbidities such as HIV and malaria. Developing a strategy to control the emergence and spread of highly invasive and antimicrobial resistant NTS isolates requires a comprehensive analysis of epidemiological factors and molecular pathogenesis. Here, we characterize 11 NTS isolates that caused bloodstream infections in pediatric patients in Siaya, Kenya from 2003-2010. Nine isolates were identified as S. Typhimurium sequence type 313 while the other two were S. Enteritidis. Comprehensive genotypic and phenotypic analyses were performed to compare these isolates to those previously identified in sub-Saharan Africa. We identified a S. Typhimurium isolate referred to as UGA14 that displayed novel plasmid, pseudogene and resistance features as compared to other isolates reported from Africa. Notably, UGA14 is able to ferment both lactose and sucrose due to the acquisition of insertion elements on the pKST313 plasmid. These findings show for the first time the co-evolution of plasmid-mediated lactose and sucrose metabolism along with cephalosporin resistance in NTS further elucidating the evolutionary mechanisms of invasive NTS phenotypes. These results further support the use of combined genomic and phenotypic approaches to detect and characterize atypical NTS isolates in order to advance biosurveillance efforts that inform countermeasures aimed at controlling invasive and antimicrobial resistant NTS. |
format |
Article in Journal/Newspaper |
author |
Jessica Z Kubicek-Sutherland Gary Xie Migun Shakya Priya K Dighe Lindsey L Jacobs Hajnalka Daligault Karen Davenport Loreen R Stromberg Zachary R Stromberg Qiuying Cheng Prakasha Kempaiah John Michael Ong'echa Vincent Otieno Evans Raballah Samuel Anyona Collins Ouma Patrick S G Chain Douglas J Perkins Harshini Mukundan Benjamin H McMahon Norman A Doggett |
author_facet |
Jessica Z Kubicek-Sutherland Gary Xie Migun Shakya Priya K Dighe Lindsey L Jacobs Hajnalka Daligault Karen Davenport Loreen R Stromberg Zachary R Stromberg Qiuying Cheng Prakasha Kempaiah John Michael Ong'echa Vincent Otieno Evans Raballah Samuel Anyona Collins Ouma Patrick S G Chain Douglas J Perkins Harshini Mukundan Benjamin H McMahon Norman A Doggett |
author_sort |
Jessica Z Kubicek-Sutherland |
title |
Comparative genomic and phenotypic characterization of invasive non-typhoidal Salmonella isolates from Siaya, Kenya. |
title_short |
Comparative genomic and phenotypic characterization of invasive non-typhoidal Salmonella isolates from Siaya, Kenya. |
title_full |
Comparative genomic and phenotypic characterization of invasive non-typhoidal Salmonella isolates from Siaya, Kenya. |
title_fullStr |
Comparative genomic and phenotypic characterization of invasive non-typhoidal Salmonella isolates from Siaya, Kenya. |
title_full_unstemmed |
Comparative genomic and phenotypic characterization of invasive non-typhoidal Salmonella isolates from Siaya, Kenya. |
title_sort |
comparative genomic and phenotypic characterization of invasive non-typhoidal salmonella isolates from siaya, kenya. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0008991 https://doaj.org/article/0316fd1fe8794975a6b88fbd139a1722 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 2, p e0008991 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008991 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008991 https://doaj.org/article/0316fd1fe8794975a6b88fbd139a1722 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008991 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
15 |
container_issue |
2 |
container_start_page |
e0008991 |
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1766340467700531200 |