Differential cytokine gene expression according to outcome in a hamster model of leptospirosis.
BACKGROUND: Parameters predicting the evolution of leptospirosis would be useful for clinicians, as well as to better understand severe leptospirosis, but are scarce and rarely validated. Because severe leptospirosis includes septic shock, similarities with predictors evidenced for sepsis and septic...
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ftdoajarticles:oai:doaj.org/article:0303c4a4b2ac43c583ffd0af24955a7d 2023-05-15T15:09:20+02:00 Differential cytokine gene expression according to outcome in a hamster model of leptospirosis. Frédérique Vernel-Pauillac Cyrille Goarant 2010-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000582 https://doaj.org/article/0303c4a4b2ac43c583ffd0af24955a7d EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2797601?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 doi:10.1371/journal.pntd.0000582 1935-2727 1935-2735 https://doaj.org/article/0303c4a4b2ac43c583ffd0af24955a7d PLoS Neglected Tropical Diseases, Vol 4, Iss 1, p e582 (2010) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2010 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000582 2022-12-31T00:56:24Z BACKGROUND: Parameters predicting the evolution of leptospirosis would be useful for clinicians, as well as to better understand severe leptospirosis, but are scarce and rarely validated. Because severe leptospirosis includes septic shock, similarities with predictors evidenced for sepsis and septic shock were studied in a hamster model. METHODOLOGY/PRINCIPAL FINDINGS: Using an LD50 model of leptospirosis in hamsters, we first determined that 3 days post-infection was a time-point that allowed studying the regulation of immune gene expression and represented the onset of the clinical signs of the disease. In the absence of tools to assess serum concentrations of immune effectors in hamsters, we determined mRNA levels of various immune genes, especially cytokines, together with leptospiraemia at this particular time-point. We found differential expression of both pro- and anti-inflammatory mediators, with significantly higher expression levels of tumor necrosis factor alpha, interleukin 1alpha, cyclo-oxygenase 2 and interleukin 10 genes in nonsurvivors compared to survivors. Higher leptospiraemia was also observed in nonsurvivors. Lastly, we demonstrated the relevance of these results by comparing their respective expression levels using a LD100 model or an isogenic high-passage nonvirulent variant. CONCLUSIONS/SIGNIFICANCE: Up-regulated gene expression of both pro- and anti-inflammatory immune effectors in hamsters with fatal outcome in an LD50 model of leptospirosis, together with a higher Leptospira burden, suggest that these gene expression levels could be predictors of adverse outcome in leptospirosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 4 1 e582 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Frédérique Vernel-Pauillac Cyrille Goarant Differential cytokine gene expression according to outcome in a hamster model of leptospirosis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Parameters predicting the evolution of leptospirosis would be useful for clinicians, as well as to better understand severe leptospirosis, but are scarce and rarely validated. Because severe leptospirosis includes septic shock, similarities with predictors evidenced for sepsis and septic shock were studied in a hamster model. METHODOLOGY/PRINCIPAL FINDINGS: Using an LD50 model of leptospirosis in hamsters, we first determined that 3 days post-infection was a time-point that allowed studying the regulation of immune gene expression and represented the onset of the clinical signs of the disease. In the absence of tools to assess serum concentrations of immune effectors in hamsters, we determined mRNA levels of various immune genes, especially cytokines, together with leptospiraemia at this particular time-point. We found differential expression of both pro- and anti-inflammatory mediators, with significantly higher expression levels of tumor necrosis factor alpha, interleukin 1alpha, cyclo-oxygenase 2 and interleukin 10 genes in nonsurvivors compared to survivors. Higher leptospiraemia was also observed in nonsurvivors. Lastly, we demonstrated the relevance of these results by comparing their respective expression levels using a LD100 model or an isogenic high-passage nonvirulent variant. CONCLUSIONS/SIGNIFICANCE: Up-regulated gene expression of both pro- and anti-inflammatory immune effectors in hamsters with fatal outcome in an LD50 model of leptospirosis, together with a higher Leptospira burden, suggest that these gene expression levels could be predictors of adverse outcome in leptospirosis. |
format |
Article in Journal/Newspaper |
author |
Frédérique Vernel-Pauillac Cyrille Goarant |
author_facet |
Frédérique Vernel-Pauillac Cyrille Goarant |
author_sort |
Frédérique Vernel-Pauillac |
title |
Differential cytokine gene expression according to outcome in a hamster model of leptospirosis. |
title_short |
Differential cytokine gene expression according to outcome in a hamster model of leptospirosis. |
title_full |
Differential cytokine gene expression according to outcome in a hamster model of leptospirosis. |
title_fullStr |
Differential cytokine gene expression according to outcome in a hamster model of leptospirosis. |
title_full_unstemmed |
Differential cytokine gene expression according to outcome in a hamster model of leptospirosis. |
title_sort |
differential cytokine gene expression according to outcome in a hamster model of leptospirosis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2010 |
url |
https://doi.org/10.1371/journal.pntd.0000582 https://doaj.org/article/0303c4a4b2ac43c583ffd0af24955a7d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 4, Iss 1, p e582 (2010) |
op_relation |
http://europepmc.org/articles/PMC2797601?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 doi:10.1371/journal.pntd.0000582 1935-2727 1935-2735 https://doaj.org/article/0303c4a4b2ac43c583ffd0af24955a7d |
op_doi |
https://doi.org/10.1371/journal.pntd.0000582 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
4 |
container_issue |
1 |
container_start_page |
e582 |
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1766340551792132096 |