Depleted circulatory complement-lysis inhibitor (CLI) in childhood cerebral malaria returns to normal with convalescence
Abstract Background Cerebral malaria (CM), is a life-threatening childhood malaria syndrome with high mortality. CM is associated with impaired consciousness and neurological damage. It is not fully understood, as yet, why some children develop CM. Presented here is an observation from longitudinal...
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ftdoajarticles:oai:doaj.org/article:02dff663acb44c5ebabf52fa07cf1cab 2023-05-15T15:13:15+02:00 Depleted circulatory complement-lysis inhibitor (CLI) in childhood cerebral malaria returns to normal with convalescence Samuel Eneọjọ Abah Florence Burté Steven A. Howell Ikeoluwa Lagunju Wuraola A. Shokunbi Mats Wahlgren Olugbemiro Sodeinde Biobele J. Brown Anthony A. Holder Delmiro Fernandez-Reyes 2020-04-01T00:00:00Z https://doi.org/10.1186/s12936-020-03241-5 https://doaj.org/article/02dff663acb44c5ebabf52fa07cf1cab EN eng BMC http://link.springer.com/article/10.1186/s12936-020-03241-5 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03241-5 1475-2875 https://doaj.org/article/02dff663acb44c5ebabf52fa07cf1cab Malaria Journal, Vol 19, Iss 1, Pp 1-11 (2020) Pathogenesis Childhood severe malaria Cerebral malaria Biomarkers Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-020-03241-5 2022-12-31T15:57:21Z Abstract Background Cerebral malaria (CM), is a life-threatening childhood malaria syndrome with high mortality. CM is associated with impaired consciousness and neurological damage. It is not fully understood, as yet, why some children develop CM. Presented here is an observation from longitudinal studies on CM in a paediatric cohort of children from a large, densely-populated and malaria holoendemic, sub-Saharan, West African metropolis. Methods Plasma samples were collected from a cohort of children with CM, severe malarial anaemia (SMA), uncomplicated malaria (UM), non-malaria positive healthy community controls (CC), and coma and anemic patients without malaria, as disease controls (DC). Proteomic two-dimensional difference gel electrophoresis (2D-DIGE) and mass spectrometry were used in a discovery cohort to identify plasma proteins that might be discriminatory among these clinical groups. The circulatory levels of identified proteins of interest were quantified by ELISA in a prospective validation cohort. Results The proteome analysis revealed differential abundance of circulatory complement-lysis inhibitor (CLI), also known as Clusterin (CLU). CLI circulatory level was low at hospital admission in all children presenting with CM and recovered to normal level during convalescence (p < 0.0001). At acute onset, circulatory level of CLI in the CM group significantly discriminates CM from the UM, SMA, DC and CC groups. Conclusions The CLI circulatory level is low in all patients in the CM group at admission, but recovers through convalescence. The level of CLI at acute onset may be a specific discriminatory marker of CM. This work suggests that CLI may play a role in the pathophysiology of CM and may be useful in the diagnosis and follow-up of children presenting with CM. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 19 1 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Pathogenesis Childhood severe malaria Cerebral malaria Biomarkers Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Pathogenesis Childhood severe malaria Cerebral malaria Biomarkers Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Samuel Eneọjọ Abah Florence Burté Steven A. Howell Ikeoluwa Lagunju Wuraola A. Shokunbi Mats Wahlgren Olugbemiro Sodeinde Biobele J. Brown Anthony A. Holder Delmiro Fernandez-Reyes Depleted circulatory complement-lysis inhibitor (CLI) in childhood cerebral malaria returns to normal with convalescence |
topic_facet |
Pathogenesis Childhood severe malaria Cerebral malaria Biomarkers Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Cerebral malaria (CM), is a life-threatening childhood malaria syndrome with high mortality. CM is associated with impaired consciousness and neurological damage. It is not fully understood, as yet, why some children develop CM. Presented here is an observation from longitudinal studies on CM in a paediatric cohort of children from a large, densely-populated and malaria holoendemic, sub-Saharan, West African metropolis. Methods Plasma samples were collected from a cohort of children with CM, severe malarial anaemia (SMA), uncomplicated malaria (UM), non-malaria positive healthy community controls (CC), and coma and anemic patients without malaria, as disease controls (DC). Proteomic two-dimensional difference gel electrophoresis (2D-DIGE) and mass spectrometry were used in a discovery cohort to identify plasma proteins that might be discriminatory among these clinical groups. The circulatory levels of identified proteins of interest were quantified by ELISA in a prospective validation cohort. Results The proteome analysis revealed differential abundance of circulatory complement-lysis inhibitor (CLI), also known as Clusterin (CLU). CLI circulatory level was low at hospital admission in all children presenting with CM and recovered to normal level during convalescence (p < 0.0001). At acute onset, circulatory level of CLI in the CM group significantly discriminates CM from the UM, SMA, DC and CC groups. Conclusions The CLI circulatory level is low in all patients in the CM group at admission, but recovers through convalescence. The level of CLI at acute onset may be a specific discriminatory marker of CM. This work suggests that CLI may play a role in the pathophysiology of CM and may be useful in the diagnosis and follow-up of children presenting with CM. |
format |
Article in Journal/Newspaper |
author |
Samuel Eneọjọ Abah Florence Burté Steven A. Howell Ikeoluwa Lagunju Wuraola A. Shokunbi Mats Wahlgren Olugbemiro Sodeinde Biobele J. Brown Anthony A. Holder Delmiro Fernandez-Reyes |
author_facet |
Samuel Eneọjọ Abah Florence Burté Steven A. Howell Ikeoluwa Lagunju Wuraola A. Shokunbi Mats Wahlgren Olugbemiro Sodeinde Biobele J. Brown Anthony A. Holder Delmiro Fernandez-Reyes |
author_sort |
Samuel Eneọjọ Abah |
title |
Depleted circulatory complement-lysis inhibitor (CLI) in childhood cerebral malaria returns to normal with convalescence |
title_short |
Depleted circulatory complement-lysis inhibitor (CLI) in childhood cerebral malaria returns to normal with convalescence |
title_full |
Depleted circulatory complement-lysis inhibitor (CLI) in childhood cerebral malaria returns to normal with convalescence |
title_fullStr |
Depleted circulatory complement-lysis inhibitor (CLI) in childhood cerebral malaria returns to normal with convalescence |
title_full_unstemmed |
Depleted circulatory complement-lysis inhibitor (CLI) in childhood cerebral malaria returns to normal with convalescence |
title_sort |
depleted circulatory complement-lysis inhibitor (cli) in childhood cerebral malaria returns to normal with convalescence |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-020-03241-5 https://doaj.org/article/02dff663acb44c5ebabf52fa07cf1cab |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-11 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s12936-020-03241-5 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03241-5 1475-2875 https://doaj.org/article/02dff663acb44c5ebabf52fa07cf1cab |
op_doi |
https://doi.org/10.1186/s12936-020-03241-5 |
container_title |
Malaria Journal |
container_volume |
19 |
container_issue |
1 |
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1766343835016757248 |