Induction of HO-1 in tissue macrophages and monocytes in fatal falciparum malaria and sepsis
Abstract Background As well as being inducible by haem, haemoxygenase -1 (HO-1) is also induced by interleukin-10 and an anti-inflammatory prostaglandin, 15d PGJ 2 , the carbon monoxide thus produced mediating the anti-inflammatory effects of these molecules. The cellular distribution of HO-1, by im...
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ftdoajarticles:oai:doaj.org/article:02c1da5af01646f2abe421c579c6e132 2023-05-15T15:16:03+02:00 Induction of HO-1 in tissue macrophages and monocytes in fatal falciparum malaria and sepsis Liomba N Harper Clive G Awburn Melissa M Clark Ian A Molyneux Malcolm E 2003-11-01T00:00:00Z https://doi.org/10.1186/1475-2875-2-41 https://doaj.org/article/02c1da5af01646f2abe421c579c6e132 EN eng BMC http://www.malariajournal.com/content/2/1/41 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-2-41 1475-2875 https://doaj.org/article/02c1da5af01646f2abe421c579c6e132 Malaria Journal, Vol 2, Iss 1, p 41 (2003) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2003 ftdoajarticles https://doi.org/10.1186/1475-2875-2-41 2022-12-31T08:11:04Z Abstract Background As well as being inducible by haem, haemoxygenase -1 (HO-1) is also induced by interleukin-10 and an anti-inflammatory prostaglandin, 15d PGJ 2 , the carbon monoxide thus produced mediating the anti-inflammatory effects of these molecules. The cellular distribution of HO-1, by immunohistochemistry, in brain, lung and liver in fatal falciparum malaria, and in sepsis, is reported. Methods Wax sections were stained, at a 1:1000 dilution of primary antibody, for HO-1 in tissues collected during paediatric autopsies in Blantyre, Malawi. These comprised 37 acutely ill comatose patients, 32 of whom were diagnosed clinically as cerebral malaria and the other 5 as bacterial diseases with coma. Another 3 died unexpectedly from an alert state. Other control tissues were from Australian adults. Results Apart from its presence in splenic red pulp macrophages and microhaemorrhages, staining for HO-1 was confined to intravascular monocytes and certain tissue macrophages. Of the 32 clinically diagnosed cerebral malaria cases, 11 (category A) cases had negligible histological change in the brain and absence of or scanty intravascular sequestration of parasitized erythrocytes. Of these 11 cases, eight proved at autopsy to have other pathological changes as well, and none of these eight showed HO-1 staining within the brain apart from isolated moderate staining in one case. Two of the three without another pathological diagnosis showed moderate staining of scattered monocytes in brain vessels. Six of these 11 (category A) cases exhibited strong lung staining, and the Kupffer cells of nine of them were intensely stained. Of the seven (category B) cases with no histological changes in the brain, but appreciable sequestered parasitised erythrocytes present, one was without staining, and the other six showed strongly staining, rare or scattered monocytes in cerebral vessels. All six lung sections not obscured by neutrophils showed strong staining of monocytes and alveolar macrophages, and all six available liver ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 2 1 41 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Liomba N Harper Clive G Awburn Melissa M Clark Ian A Molyneux Malcolm E Induction of HO-1 in tissue macrophages and monocytes in fatal falciparum malaria and sepsis |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background As well as being inducible by haem, haemoxygenase -1 (HO-1) is also induced by interleukin-10 and an anti-inflammatory prostaglandin, 15d PGJ 2 , the carbon monoxide thus produced mediating the anti-inflammatory effects of these molecules. The cellular distribution of HO-1, by immunohistochemistry, in brain, lung and liver in fatal falciparum malaria, and in sepsis, is reported. Methods Wax sections were stained, at a 1:1000 dilution of primary antibody, for HO-1 in tissues collected during paediatric autopsies in Blantyre, Malawi. These comprised 37 acutely ill comatose patients, 32 of whom were diagnosed clinically as cerebral malaria and the other 5 as bacterial diseases with coma. Another 3 died unexpectedly from an alert state. Other control tissues were from Australian adults. Results Apart from its presence in splenic red pulp macrophages and microhaemorrhages, staining for HO-1 was confined to intravascular monocytes and certain tissue macrophages. Of the 32 clinically diagnosed cerebral malaria cases, 11 (category A) cases had negligible histological change in the brain and absence of or scanty intravascular sequestration of parasitized erythrocytes. Of these 11 cases, eight proved at autopsy to have other pathological changes as well, and none of these eight showed HO-1 staining within the brain apart from isolated moderate staining in one case. Two of the three without another pathological diagnosis showed moderate staining of scattered monocytes in brain vessels. Six of these 11 (category A) cases exhibited strong lung staining, and the Kupffer cells of nine of them were intensely stained. Of the seven (category B) cases with no histological changes in the brain, but appreciable sequestered parasitised erythrocytes present, one was without staining, and the other six showed strongly staining, rare or scattered monocytes in cerebral vessels. All six lung sections not obscured by neutrophils showed strong staining of monocytes and alveolar macrophages, and all six available liver ... |
format |
Article in Journal/Newspaper |
author |
Liomba N Harper Clive G Awburn Melissa M Clark Ian A Molyneux Malcolm E |
author_facet |
Liomba N Harper Clive G Awburn Melissa M Clark Ian A Molyneux Malcolm E |
author_sort |
Liomba N |
title |
Induction of HO-1 in tissue macrophages and monocytes in fatal falciparum malaria and sepsis |
title_short |
Induction of HO-1 in tissue macrophages and monocytes in fatal falciparum malaria and sepsis |
title_full |
Induction of HO-1 in tissue macrophages and monocytes in fatal falciparum malaria and sepsis |
title_fullStr |
Induction of HO-1 in tissue macrophages and monocytes in fatal falciparum malaria and sepsis |
title_full_unstemmed |
Induction of HO-1 in tissue macrophages and monocytes in fatal falciparum malaria and sepsis |
title_sort |
induction of ho-1 in tissue macrophages and monocytes in fatal falciparum malaria and sepsis |
publisher |
BMC |
publishDate |
2003 |
url |
https://doi.org/10.1186/1475-2875-2-41 https://doaj.org/article/02c1da5af01646f2abe421c579c6e132 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 2, Iss 1, p 41 (2003) |
op_relation |
http://www.malariajournal.com/content/2/1/41 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-2-41 1475-2875 https://doaj.org/article/02c1da5af01646f2abe421c579c6e132 |
op_doi |
https://doi.org/10.1186/1475-2875-2-41 |
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Malaria Journal |
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2 |
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41 |
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