Brugia malayi excreted/secreted proteins at the host/parasite interface: stage- and gender-specific proteomic profiling.
Relatively little is known about the filarial proteins that interact with the human host. Although the filarial genome has recently been completed, protein profiles have been limited to only a few recombinants or purified proteins of interest. Here, we describe a large-scale proteomic analysis using...
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ftdoajarticles:oai:doaj.org/article:029206dcdf0e4bad824179152ef196cf 2023-05-15T15:16:28+02:00 Brugia malayi excreted/secreted proteins at the host/parasite interface: stage- and gender-specific proteomic profiling. Sasisekhar Bennuru Roshanak Semnani Zhaojing Meng Jose M C Ribeiro Timothy D Veenstra Thomas B Nutman 2009-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000410 https://doaj.org/article/029206dcdf0e4bad824179152ef196cf EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2659452?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000410 https://doaj.org/article/029206dcdf0e4bad824179152ef196cf PLoS Neglected Tropical Diseases, Vol 3, Iss 4, p e410 (2009) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2009 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000410 2022-12-31T14:05:48Z Relatively little is known about the filarial proteins that interact with the human host. Although the filarial genome has recently been completed, protein profiles have been limited to only a few recombinants or purified proteins of interest. Here, we describe a large-scale proteomic analysis using microcapillary reverse-phase liquid chromatography-tandem-mass spectrometry to identify the excretory-secretory (ES) products of the L3, L3 to L4 molting ES, adult male, adult female, and microfilarial stages of the filarial parasite Brugia malayi. The analysis of the ES products from adult male, adult female, microfilariae (Mf), L3, and molting L3 larvae identified 852 proteins. Annotation suggests that the functional and component distribution was very similar across each of the stages studied; however, the Mf contributed a higher proportion to the total number of identified proteins than the other stages. Of the 852 proteins identified in the ES, only 229 had previous confirmatory expressed sequence tags (ESTs) in the available databases. Moreover, this analysis was able to confirm the presence of 274 "hypothetical" proteins inferred from gene prediction algorithms applied to the B. malayi (Bm) genome. Not surprisingly, the majority (160/274) of these "hypothetical" proteins were predicted to be secreted by Signal IP and/or SecretomeP 2.0 analysis. Of major interest is the abundance of previously characterized immunomodulatory proteins such as ES-62 (leucyl aminopeptidase), MIF-1, SERPIN, glutathione peroxidase, and galectin in the ES of microfilariae (and Mf-containing adult females) compared to the adult males. In addition, searching the ES protein spectra against the Wolbachia database resulted in the identification of 90 Wolbachia-specific proteins, most of which were metabolic enzymes that have not been shown to be immunogenic. This proteomic analysis extends our knowledge of the ES and provides insight into the host-parasite interaction. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 3 4 e410 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Sasisekhar Bennuru Roshanak Semnani Zhaojing Meng Jose M C Ribeiro Timothy D Veenstra Thomas B Nutman Brugia malayi excreted/secreted proteins at the host/parasite interface: stage- and gender-specific proteomic profiling. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Relatively little is known about the filarial proteins that interact with the human host. Although the filarial genome has recently been completed, protein profiles have been limited to only a few recombinants or purified proteins of interest. Here, we describe a large-scale proteomic analysis using microcapillary reverse-phase liquid chromatography-tandem-mass spectrometry to identify the excretory-secretory (ES) products of the L3, L3 to L4 molting ES, adult male, adult female, and microfilarial stages of the filarial parasite Brugia malayi. The analysis of the ES products from adult male, adult female, microfilariae (Mf), L3, and molting L3 larvae identified 852 proteins. Annotation suggests that the functional and component distribution was very similar across each of the stages studied; however, the Mf contributed a higher proportion to the total number of identified proteins than the other stages. Of the 852 proteins identified in the ES, only 229 had previous confirmatory expressed sequence tags (ESTs) in the available databases. Moreover, this analysis was able to confirm the presence of 274 "hypothetical" proteins inferred from gene prediction algorithms applied to the B. malayi (Bm) genome. Not surprisingly, the majority (160/274) of these "hypothetical" proteins were predicted to be secreted by Signal IP and/or SecretomeP 2.0 analysis. Of major interest is the abundance of previously characterized immunomodulatory proteins such as ES-62 (leucyl aminopeptidase), MIF-1, SERPIN, glutathione peroxidase, and galectin in the ES of microfilariae (and Mf-containing adult females) compared to the adult males. In addition, searching the ES protein spectra against the Wolbachia database resulted in the identification of 90 Wolbachia-specific proteins, most of which were metabolic enzymes that have not been shown to be immunogenic. This proteomic analysis extends our knowledge of the ES and provides insight into the host-parasite interaction. |
format |
Article in Journal/Newspaper |
author |
Sasisekhar Bennuru Roshanak Semnani Zhaojing Meng Jose M C Ribeiro Timothy D Veenstra Thomas B Nutman |
author_facet |
Sasisekhar Bennuru Roshanak Semnani Zhaojing Meng Jose M C Ribeiro Timothy D Veenstra Thomas B Nutman |
author_sort |
Sasisekhar Bennuru |
title |
Brugia malayi excreted/secreted proteins at the host/parasite interface: stage- and gender-specific proteomic profiling. |
title_short |
Brugia malayi excreted/secreted proteins at the host/parasite interface: stage- and gender-specific proteomic profiling. |
title_full |
Brugia malayi excreted/secreted proteins at the host/parasite interface: stage- and gender-specific proteomic profiling. |
title_fullStr |
Brugia malayi excreted/secreted proteins at the host/parasite interface: stage- and gender-specific proteomic profiling. |
title_full_unstemmed |
Brugia malayi excreted/secreted proteins at the host/parasite interface: stage- and gender-specific proteomic profiling. |
title_sort |
brugia malayi excreted/secreted proteins at the host/parasite interface: stage- and gender-specific proteomic profiling. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2009 |
url |
https://doi.org/10.1371/journal.pntd.0000410 https://doaj.org/article/029206dcdf0e4bad824179152ef196cf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 3, Iss 4, p e410 (2009) |
op_relation |
http://europepmc.org/articles/PMC2659452?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000410 https://doaj.org/article/029206dcdf0e4bad824179152ef196cf |
op_doi |
https://doi.org/10.1371/journal.pntd.0000410 |
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PLoS Neglected Tropical Diseases |
container_volume |
3 |
container_issue |
4 |
container_start_page |
e410 |
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1766346771585302528 |