Antimicrobial effect of Malaysian vegetables against enteric bacteria
Objective: To investigate the antibacterial activities of green vegetables (pennywort, mint, garlic, parsley and celery) against four common enteric bacteria [Salmonella enterica (ATCC 25957) (S. enterica), Shigella flexneri (ATCC 12022) (S. flexneri), Escherichia coli (ATCC 43889) (E. coli) and Ent...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2016
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Online Access: | https://doi.org/10.1016/j.apjtb.2015.12.009 https://doaj.org/article/026ac6d60b0f43e7a50c13a1bcb55c01 |
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ftdoajarticles:oai:doaj.org/article:026ac6d60b0f43e7a50c13a1bcb55c01 2023-05-15T15:14:43+02:00 Antimicrobial effect of Malaysian vegetables against enteric bacteria Hassanain Al-Talib Norliana Dalila Mohamad Ali Mohamed Harreez Suhaimi Siti Shafika Nabila Rosli Nurul Huda Othman Nur Ain Sakinah Mansor Amira Kartini Sulaiman Shah Nurul Syuhada Ariffin Alyaa Al-Khateeb 2016-03-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2015.12.009 https://doaj.org/article/026ac6d60b0f43e7a50c13a1bcb55c01 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115309254 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.12.009 https://doaj.org/article/026ac6d60b0f43e7a50c13a1bcb55c01 Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 3, Pp 211-215 (2016) Antibacterial activities Green vegetables Enteric bacteria Inhibitory effects Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2016 ftdoajarticles https://doi.org/10.1016/j.apjtb.2015.12.009 2022-12-31T14:14:31Z Objective: To investigate the antibacterial activities of green vegetables (pennywort, mint, garlic, parsley and celery) against four common enteric bacteria [Salmonella enterica (ATCC 25957) (S. enterica), Shigella flexneri (ATCC 12022) (S. flexneri), Escherichia coli (ATCC 43889) (E. coli) and Enterobacter cloacae (ATCC 13047) (E. cloacae)] as an alternative medicine for controlling food borne diarrhea disease and the synergistic effect of green vegetables against those bacteria. Methods: Five common vegetables (pennywort, mint, garlic, parsley and celery) were purchased and extracted. The antimicrobial activities of these extracts were tested against four common enteric bacteria (S. enterica, S. flexneri, E. coli and E. cloacae). Ten different concentrations of the extracts (from 640 to 1.25 mg/mL) were prepared and used for the study. The minimal inhibitory concentration (MIC) was determined by the broth dilution method. The antimicrobial activities were assessed by using both well diffusion and disc diffusion methods. Results: Garlic extract showed excellent inhibitory effects on all enteric bacteria. Other plants (parsley, celery, mint and pennywort) were not effective against enteric bacteria. The MIC of garlic against S. flexneri and E. cloacae was 40 mg/mL. The MIC of S. enterica and E. coli were 20 and 10 mg/mL, respectively. The performance of the well diffusion method was better than that of the disc diffusion method with clear and sharp inhibition zones of tested bacteria against plant extracts. Conclusions: Garlic had excellent antimicrobial effects against enteric bacteria and was recommended to be given to patients with gastroenteritis. The other vegetables (pennywort, mint, parsley and celery) showed no inhibitory effects on enteric bacteria but still can be used for its richness in vitamins and fibers. The performance of the well diffusion method was better than that of the disc diffusion method in detecting the antibacterial effects of green vegetables. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 6 3 211 215 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antibacterial activities Green vegetables Enteric bacteria Inhibitory effects Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Antibacterial activities Green vegetables Enteric bacteria Inhibitory effects Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Hassanain Al-Talib Norliana Dalila Mohamad Ali Mohamed Harreez Suhaimi Siti Shafika Nabila Rosli Nurul Huda Othman Nur Ain Sakinah Mansor Amira Kartini Sulaiman Shah Nurul Syuhada Ariffin Alyaa Al-Khateeb Antimicrobial effect of Malaysian vegetables against enteric bacteria |
topic_facet |
Antibacterial activities Green vegetables Enteric bacteria Inhibitory effects Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the antibacterial activities of green vegetables (pennywort, mint, garlic, parsley and celery) against four common enteric bacteria [Salmonella enterica (ATCC 25957) (S. enterica), Shigella flexneri (ATCC 12022) (S. flexneri), Escherichia coli (ATCC 43889) (E. coli) and Enterobacter cloacae (ATCC 13047) (E. cloacae)] as an alternative medicine for controlling food borne diarrhea disease and the synergistic effect of green vegetables against those bacteria. Methods: Five common vegetables (pennywort, mint, garlic, parsley and celery) were purchased and extracted. The antimicrobial activities of these extracts were tested against four common enteric bacteria (S. enterica, S. flexneri, E. coli and E. cloacae). Ten different concentrations of the extracts (from 640 to 1.25 mg/mL) were prepared and used for the study. The minimal inhibitory concentration (MIC) was determined by the broth dilution method. The antimicrobial activities were assessed by using both well diffusion and disc diffusion methods. Results: Garlic extract showed excellent inhibitory effects on all enteric bacteria. Other plants (parsley, celery, mint and pennywort) were not effective against enteric bacteria. The MIC of garlic against S. flexneri and E. cloacae was 40 mg/mL. The MIC of S. enterica and E. coli were 20 and 10 mg/mL, respectively. The performance of the well diffusion method was better than that of the disc diffusion method with clear and sharp inhibition zones of tested bacteria against plant extracts. Conclusions: Garlic had excellent antimicrobial effects against enteric bacteria and was recommended to be given to patients with gastroenteritis. The other vegetables (pennywort, mint, parsley and celery) showed no inhibitory effects on enteric bacteria but still can be used for its richness in vitamins and fibers. The performance of the well diffusion method was better than that of the disc diffusion method in detecting the antibacterial effects of green vegetables. |
format |
Article in Journal/Newspaper |
author |
Hassanain Al-Talib Norliana Dalila Mohamad Ali Mohamed Harreez Suhaimi Siti Shafika Nabila Rosli Nurul Huda Othman Nur Ain Sakinah Mansor Amira Kartini Sulaiman Shah Nurul Syuhada Ariffin Alyaa Al-Khateeb |
author_facet |
Hassanain Al-Talib Norliana Dalila Mohamad Ali Mohamed Harreez Suhaimi Siti Shafika Nabila Rosli Nurul Huda Othman Nur Ain Sakinah Mansor Amira Kartini Sulaiman Shah Nurul Syuhada Ariffin Alyaa Al-Khateeb |
author_sort |
Hassanain Al-Talib |
title |
Antimicrobial effect of Malaysian vegetables against enteric bacteria |
title_short |
Antimicrobial effect of Malaysian vegetables against enteric bacteria |
title_full |
Antimicrobial effect of Malaysian vegetables against enteric bacteria |
title_fullStr |
Antimicrobial effect of Malaysian vegetables against enteric bacteria |
title_full_unstemmed |
Antimicrobial effect of Malaysian vegetables against enteric bacteria |
title_sort |
antimicrobial effect of malaysian vegetables against enteric bacteria |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.apjtb.2015.12.009 https://doaj.org/article/026ac6d60b0f43e7a50c13a1bcb55c01 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 3, Pp 211-215 (2016) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115309254 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.12.009 https://doaj.org/article/026ac6d60b0f43e7a50c13a1bcb55c01 |
op_doi |
https://doi.org/10.1016/j.apjtb.2015.12.009 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
6 |
container_issue |
3 |
container_start_page |
211 |
op_container_end_page |
215 |
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1766345137136336896 |