Hydrocarbon-Degrading Bacteria Found Tightly Associated with the 50–70 μm Cell-Size Population of Eukaryotic Phytoplankton in Surface Waters of a Northeast Atlantic Region
The surface of marine eukaryotic phytoplankton can harbour communities of hydrocarbon-degrading bacteria; however, this algal–bacterial association has, hitherto, been only examined with non-axenic laboratory cultures of micro-algae. In this study, we isolated an operationally-defined community of p...
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ftdoajarticles:oai:doaj.org/article:02090d28cc1248f0b4ecb983cc65c8c2 2023-05-15T17:41:15+02:00 Hydrocarbon-Degrading Bacteria Found Tightly Associated with the 50–70 μm Cell-Size Population of Eukaryotic Phytoplankton in Surface Waters of a Northeast Atlantic Region Haydn Frank Thompson Stephen Summers Raif Yuecel Tony Gutierrez 2020-12-01T00:00:00Z https://doi.org/10.3390/microorganisms8121955 https://doaj.org/article/02090d28cc1248f0b4ecb983cc65c8c2 EN eng MDPI AG https://www.mdpi.com/2076-2607/8/12/1955 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms8121955 2076-2607 https://doaj.org/article/02090d28cc1248f0b4ecb983cc65c8c2 Microorganisms, Vol 8, Iss 1955, p 1955 (2020) hydrocarbonoclastic bacteria Marinobacter eukaryotic phytoplankton micro-algae phycosphere fluorescence in situ hybridisation (FISH) Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.3390/microorganisms8121955 2022-12-31T06:44:07Z The surface of marine eukaryotic phytoplankton can harbour communities of hydrocarbon-degrading bacteria; however, this algal–bacterial association has, hitherto, been only examined with non-axenic laboratory cultures of micro-algae. In this study, we isolated an operationally-defined community of phytoplankton, of cell size 50–70 μm, from a natural community in sea surface waters of a subarctic region in the northeast Atlantic. Using MiSeq 16S rRNA sequencing, we identified several recognized ( Alcanivorax , Marinobacter , Oleispira , Porticoccus , Thalassospira ) and putative hydrocarbon degraders ( Colwelliaceae , Vibrionaceae ) tightly associated with the phytoplankton population. We combined fluorescence in situ hybridisation with flow-cytometry (FISH-Flow) to examine the association of Marinobacter with this natural eukaryotic phytoplankton population. About 1.5% of the phytoplankton population contained tightly associated Marinobacter . The remaining Marinobacter population were loosely associated with either eukaryotic phytoplankton cells or non-chlorophyll particulate material. This work is the first to show the presence of obligate, generalist and putative hydrocarbonoclastic bacteria associated with natural populations of eukaryotic phytoplankton directly from sea surface water samples. It also highlights the suitability of FISH-Flow for future studies to examine the spatial and temporal structure and dynamics of these and other algal–bacterial associations in natural seawater samples. Article in Journal/Newspaper Northeast Atlantic Subarctic Directory of Open Access Journals: DOAJ Articles Microorganisms 8 12 1955 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
hydrocarbonoclastic bacteria Marinobacter eukaryotic phytoplankton micro-algae phycosphere fluorescence in situ hybridisation (FISH) Biology (General) QH301-705.5 |
spellingShingle |
hydrocarbonoclastic bacteria Marinobacter eukaryotic phytoplankton micro-algae phycosphere fluorescence in situ hybridisation (FISH) Biology (General) QH301-705.5 Haydn Frank Thompson Stephen Summers Raif Yuecel Tony Gutierrez Hydrocarbon-Degrading Bacteria Found Tightly Associated with the 50–70 μm Cell-Size Population of Eukaryotic Phytoplankton in Surface Waters of a Northeast Atlantic Region |
topic_facet |
hydrocarbonoclastic bacteria Marinobacter eukaryotic phytoplankton micro-algae phycosphere fluorescence in situ hybridisation (FISH) Biology (General) QH301-705.5 |
description |
The surface of marine eukaryotic phytoplankton can harbour communities of hydrocarbon-degrading bacteria; however, this algal–bacterial association has, hitherto, been only examined with non-axenic laboratory cultures of micro-algae. In this study, we isolated an operationally-defined community of phytoplankton, of cell size 50–70 μm, from a natural community in sea surface waters of a subarctic region in the northeast Atlantic. Using MiSeq 16S rRNA sequencing, we identified several recognized ( Alcanivorax , Marinobacter , Oleispira , Porticoccus , Thalassospira ) and putative hydrocarbon degraders ( Colwelliaceae , Vibrionaceae ) tightly associated with the phytoplankton population. We combined fluorescence in situ hybridisation with flow-cytometry (FISH-Flow) to examine the association of Marinobacter with this natural eukaryotic phytoplankton population. About 1.5% of the phytoplankton population contained tightly associated Marinobacter . The remaining Marinobacter population were loosely associated with either eukaryotic phytoplankton cells or non-chlorophyll particulate material. This work is the first to show the presence of obligate, generalist and putative hydrocarbonoclastic bacteria associated with natural populations of eukaryotic phytoplankton directly from sea surface water samples. It also highlights the suitability of FISH-Flow for future studies to examine the spatial and temporal structure and dynamics of these and other algal–bacterial associations in natural seawater samples. |
format |
Article in Journal/Newspaper |
author |
Haydn Frank Thompson Stephen Summers Raif Yuecel Tony Gutierrez |
author_facet |
Haydn Frank Thompson Stephen Summers Raif Yuecel Tony Gutierrez |
author_sort |
Haydn Frank Thompson |
title |
Hydrocarbon-Degrading Bacteria Found Tightly Associated with the 50–70 μm Cell-Size Population of Eukaryotic Phytoplankton in Surface Waters of a Northeast Atlantic Region |
title_short |
Hydrocarbon-Degrading Bacteria Found Tightly Associated with the 50–70 μm Cell-Size Population of Eukaryotic Phytoplankton in Surface Waters of a Northeast Atlantic Region |
title_full |
Hydrocarbon-Degrading Bacteria Found Tightly Associated with the 50–70 μm Cell-Size Population of Eukaryotic Phytoplankton in Surface Waters of a Northeast Atlantic Region |
title_fullStr |
Hydrocarbon-Degrading Bacteria Found Tightly Associated with the 50–70 μm Cell-Size Population of Eukaryotic Phytoplankton in Surface Waters of a Northeast Atlantic Region |
title_full_unstemmed |
Hydrocarbon-Degrading Bacteria Found Tightly Associated with the 50–70 μm Cell-Size Population of Eukaryotic Phytoplankton in Surface Waters of a Northeast Atlantic Region |
title_sort |
hydrocarbon-degrading bacteria found tightly associated with the 50–70 μm cell-size population of eukaryotic phytoplankton in surface waters of a northeast atlantic region |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/microorganisms8121955 https://doaj.org/article/02090d28cc1248f0b4ecb983cc65c8c2 |
genre |
Northeast Atlantic Subarctic |
genre_facet |
Northeast Atlantic Subarctic |
op_source |
Microorganisms, Vol 8, Iss 1955, p 1955 (2020) |
op_relation |
https://www.mdpi.com/2076-2607/8/12/1955 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms8121955 2076-2607 https://doaj.org/article/02090d28cc1248f0b4ecb983cc65c8c2 |
op_doi |
https://doi.org/10.3390/microorganisms8121955 |
container_title |
Microorganisms |
container_volume |
8 |
container_issue |
12 |
container_start_page |
1955 |
_version_ |
1766142711630397440 |