Antivenom reversal of biochemical alterations induced by black scorpion Heterometrus fastigiousus Couzijn venom in mice
In the present study, Heterometrus fastigiousus venom (HFV) was employed as antigen to produce species-specific scorpion antivenom (SAV) in albino mice (NIH) strain. To determine SAV efficacy, it was pre-incubated with 10 LD50 of HFV and then injected subcutaneously into mice. Subsequently, mortalit...
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ftdoajarticles:oai:doaj.org/article:0202918c782c4ddd987d776e118191f5 2023-05-15T15:04:17+02:00 Antivenom reversal of biochemical alterations induced by black scorpion Heterometrus fastigiousus Couzijn venom in mice MK Chaubey 2009-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992009000200006 https://doaj.org/article/0202918c782c4ddd987d776e118191f5 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200006 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992009000200006 1678-9199 https://doaj.org/article/0202918c782c4ddd987d776e118191f5 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 15, Iss 2, Pp 226-235 (2009) venom antivenom envenomation Heterometrus fastigiousus serotherapy Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2009 ftdoajarticles https://doi.org/10.1590/S1678-91992009000200006 2022-12-31T03:09:07Z In the present study, Heterometrus fastigiousus venom (HFV) was employed as antigen to produce species-specific scorpion antivenom (SAV) in albino mice (NIH) strain. To determine SAV efficacy, it was pre-incubated with 10 LD50 of HFV and then injected subcutaneously into mice. Subsequently, mortality was observed after 24 hours. Minimum effective dose (MED) was 12.5 LD50 of HFV/mL of SAV. SAV effectiveness to reverse HFV-induced biochemical alterations in mice was analyzed by challenge method. Simultaneously, mice received subcutaneously 40% of 24-hour-LD50 of HFV and intravenously SAV. After four hours, changes in serum glucose, free amino acids, uric acids, pyruvic acid, cholesterol, total protein, alkaline phosphatase, acid phosphatase, lactic dehydrogenase and glutamate-pyruvate transaminase enzyme level were determined. Treatment with species-specific SAV resulted in the reversal of HFV-induced biochemical alterations. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Sav’ ENVELOPE(156.400,156.400,68.817,68.817) Journal of Venomous Animals and Toxins including Tropical Diseases 15 2 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
venom antivenom envenomation Heterometrus fastigiousus serotherapy Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
venom antivenom envenomation Heterometrus fastigiousus serotherapy Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 MK Chaubey Antivenom reversal of biochemical alterations induced by black scorpion Heterometrus fastigiousus Couzijn venom in mice |
topic_facet |
venom antivenom envenomation Heterometrus fastigiousus serotherapy Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
In the present study, Heterometrus fastigiousus venom (HFV) was employed as antigen to produce species-specific scorpion antivenom (SAV) in albino mice (NIH) strain. To determine SAV efficacy, it was pre-incubated with 10 LD50 of HFV and then injected subcutaneously into mice. Subsequently, mortality was observed after 24 hours. Minimum effective dose (MED) was 12.5 LD50 of HFV/mL of SAV. SAV effectiveness to reverse HFV-induced biochemical alterations in mice was analyzed by challenge method. Simultaneously, mice received subcutaneously 40% of 24-hour-LD50 of HFV and intravenously SAV. After four hours, changes in serum glucose, free amino acids, uric acids, pyruvic acid, cholesterol, total protein, alkaline phosphatase, acid phosphatase, lactic dehydrogenase and glutamate-pyruvate transaminase enzyme level were determined. Treatment with species-specific SAV resulted in the reversal of HFV-induced biochemical alterations. |
format |
Article in Journal/Newspaper |
author |
MK Chaubey |
author_facet |
MK Chaubey |
author_sort |
MK Chaubey |
title |
Antivenom reversal of biochemical alterations induced by black scorpion Heterometrus fastigiousus Couzijn venom in mice |
title_short |
Antivenom reversal of biochemical alterations induced by black scorpion Heterometrus fastigiousus Couzijn venom in mice |
title_full |
Antivenom reversal of biochemical alterations induced by black scorpion Heterometrus fastigiousus Couzijn venom in mice |
title_fullStr |
Antivenom reversal of biochemical alterations induced by black scorpion Heterometrus fastigiousus Couzijn venom in mice |
title_full_unstemmed |
Antivenom reversal of biochemical alterations induced by black scorpion Heterometrus fastigiousus Couzijn venom in mice |
title_sort |
antivenom reversal of biochemical alterations induced by black scorpion heterometrus fastigiousus couzijn venom in mice |
publisher |
SciELO |
publishDate |
2009 |
url |
https://doi.org/10.1590/S1678-91992009000200006 https://doaj.org/article/0202918c782c4ddd987d776e118191f5 |
long_lat |
ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
Arctic Sav’ |
geographic_facet |
Arctic Sav’ |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 15, Iss 2, Pp 226-235 (2009) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200006 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992009000200006 1678-9199 https://doaj.org/article/0202918c782c4ddd987d776e118191f5 |
op_doi |
https://doi.org/10.1590/S1678-91992009000200006 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
15 |
container_issue |
2 |
_version_ |
1766336087376003072 |