Measuring coral calcification under ocean acidification: methodological considerations for the 45Ca-uptake and total alkalinity anomaly technique
As the oceans become less alkaline due to rising CO2 levels, deleterious consequences are expected for calcifying corals. Predicting how coral calcification will be affected by on-going ocean acidification (OA) requires an accurate assessment of CaCO3 deposition and an understanding of the relative...
Published in: | PeerJ |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
PeerJ Inc.
2017
|
Subjects: | |
Online Access: | https://doi.org/10.7717/peerj.3749 https://doaj.org/article/017f5d9771264de0bf686f38f8481d25 |
id |
ftdoajarticles:oai:doaj.org/article:017f5d9771264de0bf686f38f8481d25 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:017f5d9771264de0bf686f38f8481d25 2024-01-07T09:45:42+01:00 Measuring coral calcification under ocean acidification: methodological considerations for the 45Ca-uptake and total alkalinity anomaly technique Stephanie Cohen Thomas Krueger Maoz Fine 2017-09-01T00:00:00Z https://doi.org/10.7717/peerj.3749 https://doaj.org/article/017f5d9771264de0bf686f38f8481d25 EN eng PeerJ Inc. https://peerj.com/articles/3749.pdf https://peerj.com/articles/3749/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.3749 2167-8359 https://doaj.org/article/017f5d9771264de0bf686f38f8481d25 PeerJ, Vol 5, p e3749 (2017) Gross calcification Net calcification Coral dissolution Biomineralisation Coral reefs Medicine R Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.7717/peerj.3749 2023-12-10T01:46:56Z As the oceans become less alkaline due to rising CO2 levels, deleterious consequences are expected for calcifying corals. Predicting how coral calcification will be affected by on-going ocean acidification (OA) requires an accurate assessment of CaCO3 deposition and an understanding of the relative importance that decreasing calcification and/or increasing dissolution play for the overall calcification budget of individual corals. Here, we assessed the compatibility of the 45Ca-uptake and total alkalinity (TA) anomaly techniques as measures of gross and net calcification (GC, NC), respectively, to determine coral calcification at pHT 8.1 and 7.5. Considering the differing buffering capacity of seawater at both pH values, we were also interested in how strongly coral calcification alters the seawater carbonate chemistry under prolonged incubation in sealed chambers, potentially interfering with physiological functioning. Our data indicate that NC estimates by TA are erroneously ∼5% and ∼21% higher than GC estimates from 45Ca for ambient and reduced pH, respectively. Considering also previous data, we show that the consistent discrepancy between both techniques across studies is not constant, but largely depends on the absolute value of CaCO3 deposition. Deriving rates of coral dissolution from the difference between NC and GC was not possible and we advocate a more direct approach for the future by simultaneously measuring skeletal calcium influx and efflux. Substantial changes in carbonate system parameters for incubation times beyond two hours in our experiment demonstrate the necessity to test and optimize experimental incubation setups when measuring coral calcification in closed systems, especially under OA conditions. Article in Journal/Newspaper Ocean acidification Directory of Open Access Journals: DOAJ Articles PeerJ 5 e3749 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Gross calcification Net calcification Coral dissolution Biomineralisation Coral reefs Medicine R Biology (General) QH301-705.5 |
spellingShingle |
Gross calcification Net calcification Coral dissolution Biomineralisation Coral reefs Medicine R Biology (General) QH301-705.5 Stephanie Cohen Thomas Krueger Maoz Fine Measuring coral calcification under ocean acidification: methodological considerations for the 45Ca-uptake and total alkalinity anomaly technique |
topic_facet |
Gross calcification Net calcification Coral dissolution Biomineralisation Coral reefs Medicine R Biology (General) QH301-705.5 |
description |
As the oceans become less alkaline due to rising CO2 levels, deleterious consequences are expected for calcifying corals. Predicting how coral calcification will be affected by on-going ocean acidification (OA) requires an accurate assessment of CaCO3 deposition and an understanding of the relative importance that decreasing calcification and/or increasing dissolution play for the overall calcification budget of individual corals. Here, we assessed the compatibility of the 45Ca-uptake and total alkalinity (TA) anomaly techniques as measures of gross and net calcification (GC, NC), respectively, to determine coral calcification at pHT 8.1 and 7.5. Considering the differing buffering capacity of seawater at both pH values, we were also interested in how strongly coral calcification alters the seawater carbonate chemistry under prolonged incubation in sealed chambers, potentially interfering with physiological functioning. Our data indicate that NC estimates by TA are erroneously ∼5% and ∼21% higher than GC estimates from 45Ca for ambient and reduced pH, respectively. Considering also previous data, we show that the consistent discrepancy between both techniques across studies is not constant, but largely depends on the absolute value of CaCO3 deposition. Deriving rates of coral dissolution from the difference between NC and GC was not possible and we advocate a more direct approach for the future by simultaneously measuring skeletal calcium influx and efflux. Substantial changes in carbonate system parameters for incubation times beyond two hours in our experiment demonstrate the necessity to test and optimize experimental incubation setups when measuring coral calcification in closed systems, especially under OA conditions. |
format |
Article in Journal/Newspaper |
author |
Stephanie Cohen Thomas Krueger Maoz Fine |
author_facet |
Stephanie Cohen Thomas Krueger Maoz Fine |
author_sort |
Stephanie Cohen |
title |
Measuring coral calcification under ocean acidification: methodological considerations for the 45Ca-uptake and total alkalinity anomaly technique |
title_short |
Measuring coral calcification under ocean acidification: methodological considerations for the 45Ca-uptake and total alkalinity anomaly technique |
title_full |
Measuring coral calcification under ocean acidification: methodological considerations for the 45Ca-uptake and total alkalinity anomaly technique |
title_fullStr |
Measuring coral calcification under ocean acidification: methodological considerations for the 45Ca-uptake and total alkalinity anomaly technique |
title_full_unstemmed |
Measuring coral calcification under ocean acidification: methodological considerations for the 45Ca-uptake and total alkalinity anomaly technique |
title_sort |
measuring coral calcification under ocean acidification: methodological considerations for the 45ca-uptake and total alkalinity anomaly technique |
publisher |
PeerJ Inc. |
publishDate |
2017 |
url |
https://doi.org/10.7717/peerj.3749 https://doaj.org/article/017f5d9771264de0bf686f38f8481d25 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
PeerJ, Vol 5, p e3749 (2017) |
op_relation |
https://peerj.com/articles/3749.pdf https://peerj.com/articles/3749/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.3749 2167-8359 https://doaj.org/article/017f5d9771264de0bf686f38f8481d25 |
op_doi |
https://doi.org/10.7717/peerj.3749 |
container_title |
PeerJ |
container_volume |
5 |
container_start_page |
e3749 |
_version_ |
1787427294607835136 |