Characteristics of hemolytic activity induced by skin secretions of the frog Kaloula pulchra hainana
Background The hemolytic activity of skin secretions obtained by stimulating the frog Kaloula pulchra hainana with diethyl ether was tested using human, cattle, rabbit, and chicken erythrocytes. The skin secretions had a significant concentration-dependent hemolytic effect on erythrocytes. The hemol...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:0179aedd9b7a44258d0eab7a245cd8d0 2023-05-15T15:05:10+02:00 Characteristics of hemolytic activity induced by skin secretions of the frog Kaloula pulchra hainana Shuangshuang Wei Tingting Chi Aiyun Meng Congwei Chen Tianchen An Manchuriga Wang Yingxia Zhang 2013-04-01T00:00:00Z https://doi.org/10.1186/1678-9199-19-9 https://doaj.org/article/0179aedd9b7a44258d0eab7a245cd8d0 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100306&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/1678-9199-19-9 https://doaj.org/article/0179aedd9b7a44258d0eab7a245cd8d0 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 19, Iss 0 (2013) Amphibian Skin secretions Hemolysis Pore-forming Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2013 ftdoajarticles https://doi.org/10.1186/1678-9199-19-9 2022-12-31T15:03:24Z Background The hemolytic activity of skin secretions obtained by stimulating the frog Kaloula pulchra hainana with diethyl ether was tested using human, cattle, rabbit, and chicken erythrocytes. The skin secretions had a significant concentration-dependent hemolytic effect on erythrocytes. The hemolytic activity of the skin secretions was studied in the presence of osmotic protectants (polyethylene glycols and carbohydrates), cations (Mg2+, Ca2+, Ba2+, Cu2+, and K+), or antioxidants (ascorbic acid, reduced glutathione, and cysteine). Results Depending on their molecular mass, osmotic protectants effectively inhibited hemolysis. The inhibition of skin hemolysis was observed after treatment with polyethylene glycols (1000, 3400, and 6000 Da). Among divalent cations, only 1 mM Cu2+ markedly inhibited hemolytic activity. Antioxidant compounds slightly reduced the hemolytic activity. Conclusions The results suggested that skin secretions of K. pulchra hainana induce a pore-forming mechanism to form pores with a diameter of 1.36-2.0 nm rather than causing oxidative damage to the erythrocyte membrane. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 19 1 9 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Amphibian Skin secretions Hemolysis Pore-forming Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Amphibian Skin secretions Hemolysis Pore-forming Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Shuangshuang Wei Tingting Chi Aiyun Meng Congwei Chen Tianchen An Manchuriga Wang Yingxia Zhang Characteristics of hemolytic activity induced by skin secretions of the frog Kaloula pulchra hainana |
topic_facet |
Amphibian Skin secretions Hemolysis Pore-forming Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Background The hemolytic activity of skin secretions obtained by stimulating the frog Kaloula pulchra hainana with diethyl ether was tested using human, cattle, rabbit, and chicken erythrocytes. The skin secretions had a significant concentration-dependent hemolytic effect on erythrocytes. The hemolytic activity of the skin secretions was studied in the presence of osmotic protectants (polyethylene glycols and carbohydrates), cations (Mg2+, Ca2+, Ba2+, Cu2+, and K+), or antioxidants (ascorbic acid, reduced glutathione, and cysteine). Results Depending on their molecular mass, osmotic protectants effectively inhibited hemolysis. The inhibition of skin hemolysis was observed after treatment with polyethylene glycols (1000, 3400, and 6000 Da). Among divalent cations, only 1 mM Cu2+ markedly inhibited hemolytic activity. Antioxidant compounds slightly reduced the hemolytic activity. Conclusions The results suggested that skin secretions of K. pulchra hainana induce a pore-forming mechanism to form pores with a diameter of 1.36-2.0 nm rather than causing oxidative damage to the erythrocyte membrane. |
format |
Article in Journal/Newspaper |
author |
Shuangshuang Wei Tingting Chi Aiyun Meng Congwei Chen Tianchen An Manchuriga Wang Yingxia Zhang |
author_facet |
Shuangshuang Wei Tingting Chi Aiyun Meng Congwei Chen Tianchen An Manchuriga Wang Yingxia Zhang |
author_sort |
Shuangshuang Wei |
title |
Characteristics of hemolytic activity induced by skin secretions of the frog Kaloula pulchra hainana |
title_short |
Characteristics of hemolytic activity induced by skin secretions of the frog Kaloula pulchra hainana |
title_full |
Characteristics of hemolytic activity induced by skin secretions of the frog Kaloula pulchra hainana |
title_fullStr |
Characteristics of hemolytic activity induced by skin secretions of the frog Kaloula pulchra hainana |
title_full_unstemmed |
Characteristics of hemolytic activity induced by skin secretions of the frog Kaloula pulchra hainana |
title_sort |
characteristics of hemolytic activity induced by skin secretions of the frog kaloula pulchra hainana |
publisher |
SciELO |
publishDate |
2013 |
url |
https://doi.org/10.1186/1678-9199-19-9 https://doaj.org/article/0179aedd9b7a44258d0eab7a245cd8d0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 19, Iss 0 (2013) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100306&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/1678-9199-19-9 https://doaj.org/article/0179aedd9b7a44258d0eab7a245cd8d0 |
op_doi |
https://doi.org/10.1186/1678-9199-19-9 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
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19 |
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1 |
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9 |
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1766336922164133888 |