Opening a can of lungworms: Molecular characterization of Dictyocaulus (Nematoda: Dictyocaulidae) infecting North American bison (Bison bison)
Dictyocaulus is a globally distributed genus of lungworms of domestic and wild ungulates. Dictyocaulus adults inhabit the bronchi, frequently causing subclinical and clinical disease, and that impacts animal health and production. North American bison (Bison bison) and cattle (Bos taurus) share vari...
Published in: | International Journal for Parasitology: Parasites and Wildlife |
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Online Access: | https://doi.org/10.1016/j.ijppaw.2022.04.011 https://doaj.org/article/01587d275ea54f41b8099ce065f38d06 |
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ftdoajarticles:oai:doaj.org/article:01587d275ea54f41b8099ce065f38d06 2023-05-15T18:49:30+02:00 Opening a can of lungworms: Molecular characterization of Dictyocaulus (Nematoda: Dictyocaulidae) infecting North American bison (Bison bison) Hannah A.Danks Caroline Sobotyk Meriam N.Saleh Matthew Kulpa Joe L.Luksovsky Lee C Jones Guilherme G. Verocai 2022-08-01T00:00:00Z https://doi.org/10.1016/j.ijppaw.2022.04.011 https://doaj.org/article/01587d275ea54f41b8099ce065f38d06 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2213224422000438 https://doaj.org/toc/2213-2244 2213-2244 doi:10.1016/j.ijppaw.2022.04.011 https://doaj.org/article/01587d275ea54f41b8099ce065f38d06 International Journal for Parasitology: Parasites and Wildlife, Vol 18, Iss , Pp 128-134 (2022) Bison bison dictyocaulosis Nearctic fauna Phylogenetic relationships Verminous pneumonia Lungworm Zoology QL1-991 article 2022 ftdoajarticles https://doi.org/10.1016/j.ijppaw.2022.04.011 2022-12-30T22:37:36Z Dictyocaulus is a globally distributed genus of lungworms of domestic and wild ungulates. Dictyocaulus adults inhabit the bronchi, frequently causing subclinical and clinical disease, and that impacts animal health and production. North American bison (Bison bison) and cattle (Bos taurus) share various parasitic nematode species, particularly in areas where co-grazing occurs. The current assumption is that North American bison share the lungworm D. viviparus with cattle, but this has not been confirmed on a molecular basis. The aim of this study was to molecularly characterize Dictyocaulus lungworm isolates from North American plains bison (Bison bison bison). Fecal samples were collected from 5 wild conservation bison herds located in Iowa, North Dakota, Oklahoma, Colorado, and Montana in 2019 and 2020, and from ranched and feedlot bison from 2 herds in Oklahoma and Texas. First-stage lungworm larvae (L1) were isolated via Baermann technique. Genomic DNA was extracted from L1s of up to 3 samples per herd and followed by PCR and sequencing targeting the internal transcribed spacer 2 (ITS2) region of the nuclear ribosomal DNA and the partial cytochrome oxidase c subunit 1 (cox1) of mitochondrial DNA. Phylogenetic analyses were performed in MEGA X 10.1. Sequences of North American plains bison Dictyocaulus belong to a single, uncharacterized species, clustering in well-supported clades (100% and 100% bootstrap support for ITS2 and cox1, respectively), differing from D. viviparus of cattle in North America and Europe, and European bison (Bison bonasus). Our results contradict previous assumptions regarding parasite identity, highlighting the need for characterization of this species through morphological and molecular methods, elucidating its biology and host range, and potential impact on host health. Further investigation into the biodiversity of Dictyocaulus species infecting bovids and cervids in North America is warranted. Article in Journal/Newspaper Bison bison bison Plains Bison Directory of Open Access Journals: DOAJ Articles International Journal for Parasitology: Parasites and Wildlife 18 128 134 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Bison bison dictyocaulosis Nearctic fauna Phylogenetic relationships Verminous pneumonia Lungworm Zoology QL1-991 |
spellingShingle |
Bison bison dictyocaulosis Nearctic fauna Phylogenetic relationships Verminous pneumonia Lungworm Zoology QL1-991 Hannah A.Danks Caroline Sobotyk Meriam N.Saleh Matthew Kulpa Joe L.Luksovsky Lee C Jones Guilherme G. Verocai Opening a can of lungworms: Molecular characterization of Dictyocaulus (Nematoda: Dictyocaulidae) infecting North American bison (Bison bison) |
topic_facet |
Bison bison dictyocaulosis Nearctic fauna Phylogenetic relationships Verminous pneumonia Lungworm Zoology QL1-991 |
description |
Dictyocaulus is a globally distributed genus of lungworms of domestic and wild ungulates. Dictyocaulus adults inhabit the bronchi, frequently causing subclinical and clinical disease, and that impacts animal health and production. North American bison (Bison bison) and cattle (Bos taurus) share various parasitic nematode species, particularly in areas where co-grazing occurs. The current assumption is that North American bison share the lungworm D. viviparus with cattle, but this has not been confirmed on a molecular basis. The aim of this study was to molecularly characterize Dictyocaulus lungworm isolates from North American plains bison (Bison bison bison). Fecal samples were collected from 5 wild conservation bison herds located in Iowa, North Dakota, Oklahoma, Colorado, and Montana in 2019 and 2020, and from ranched and feedlot bison from 2 herds in Oklahoma and Texas. First-stage lungworm larvae (L1) were isolated via Baermann technique. Genomic DNA was extracted from L1s of up to 3 samples per herd and followed by PCR and sequencing targeting the internal transcribed spacer 2 (ITS2) region of the nuclear ribosomal DNA and the partial cytochrome oxidase c subunit 1 (cox1) of mitochondrial DNA. Phylogenetic analyses were performed in MEGA X 10.1. Sequences of North American plains bison Dictyocaulus belong to a single, uncharacterized species, clustering in well-supported clades (100% and 100% bootstrap support for ITS2 and cox1, respectively), differing from D. viviparus of cattle in North America and Europe, and European bison (Bison bonasus). Our results contradict previous assumptions regarding parasite identity, highlighting the need for characterization of this species through morphological and molecular methods, elucidating its biology and host range, and potential impact on host health. Further investigation into the biodiversity of Dictyocaulus species infecting bovids and cervids in North America is warranted. |
format |
Article in Journal/Newspaper |
author |
Hannah A.Danks Caroline Sobotyk Meriam N.Saleh Matthew Kulpa Joe L.Luksovsky Lee C Jones Guilherme G. Verocai |
author_facet |
Hannah A.Danks Caroline Sobotyk Meriam N.Saleh Matthew Kulpa Joe L.Luksovsky Lee C Jones Guilherme G. Verocai |
author_sort |
Hannah A.Danks |
title |
Opening a can of lungworms: Molecular characterization of Dictyocaulus (Nematoda: Dictyocaulidae) infecting North American bison (Bison bison) |
title_short |
Opening a can of lungworms: Molecular characterization of Dictyocaulus (Nematoda: Dictyocaulidae) infecting North American bison (Bison bison) |
title_full |
Opening a can of lungworms: Molecular characterization of Dictyocaulus (Nematoda: Dictyocaulidae) infecting North American bison (Bison bison) |
title_fullStr |
Opening a can of lungworms: Molecular characterization of Dictyocaulus (Nematoda: Dictyocaulidae) infecting North American bison (Bison bison) |
title_full_unstemmed |
Opening a can of lungworms: Molecular characterization of Dictyocaulus (Nematoda: Dictyocaulidae) infecting North American bison (Bison bison) |
title_sort |
opening a can of lungworms: molecular characterization of dictyocaulus (nematoda: dictyocaulidae) infecting north american bison (bison bison) |
publisher |
Elsevier |
publishDate |
2022 |
url |
https://doi.org/10.1016/j.ijppaw.2022.04.011 https://doaj.org/article/01587d275ea54f41b8099ce065f38d06 |
genre |
Bison bison bison Plains Bison |
genre_facet |
Bison bison bison Plains Bison |
op_source |
International Journal for Parasitology: Parasites and Wildlife, Vol 18, Iss , Pp 128-134 (2022) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2213224422000438 https://doaj.org/toc/2213-2244 2213-2244 doi:10.1016/j.ijppaw.2022.04.011 https://doaj.org/article/01587d275ea54f41b8099ce065f38d06 |
op_doi |
https://doi.org/10.1016/j.ijppaw.2022.04.011 |
container_title |
International Journal for Parasitology: Parasites and Wildlife |
container_volume |
18 |
container_start_page |
128 |
op_container_end_page |
134 |
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1766243090500157440 |