Investigation of class 1 integrons in Klebsiella pneumoniae clinical and microbiota isolates belonging to different phylogenetic groups in Recife, State of Pernambuco
Introduction The high prevalence of Klebsiella pneumoniae infections is related to the ability of K. pneumoniae to acquire and disseminate exogenous genes associated with mobile elements, such as R plasmids, transposons and integrons. This study investigated the presence of class 1 integrons in clin...
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ftdoajarticles:oai:doaj.org/article:015356d079d54308acb6100d1cbe48d6 2023-05-15T15:13:24+02:00 Investigation of class 1 integrons in Klebsiella pneumoniae clinical and microbiota isolates belonging to different phylogenetic groups in Recife, State of Pernambuco Alexsandra Maria Silva Lima Maíra Espíndola Silva de Melo Luiz Carlos Alves Fábio André Brayner Ana Catarina Souza Lopes 2014-04-01T00:00:00Z https://doi.org/10.1590/0037-8682-0021-2014 https://doaj.org/article/015356d079d54308acb6100d1cbe48d6 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000200165&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0021-2014 https://doaj.org/article/015356d079d54308acb6100d1cbe48d6 Revista da Sociedade Brasileira de Medicina Tropical, Vol 47, Iss 2, Pp 165-169 (2014) Class 1 integron Klebsiella pneumonia Phylogenetic groups Arctic medicine. Tropical medicine RC955-962 article 2014 ftdoajarticles https://doi.org/10.1590/0037-8682-0021-2014 2022-12-30T23:18:21Z Introduction The high prevalence of Klebsiella pneumoniae infections is related to the ability of K. pneumoniae to acquire and disseminate exogenous genes associated with mobile elements, such as R plasmids, transposons and integrons. This study investigated the presence of class 1 integrons in clinical and microbiota isolates of K. pneumoniae belonging to different phylogenetic groups and correlated these results with the antimicrobial resistance profiles of the studied isolates. Methods Of the 51 isolates of K. pneumoniae selected for this study, 29 were from multidrug-resistant clinical isolates, and 22 were from children's microbiota. The susceptibility profile was determined using the disk diffusion method, and class 1 integrons were detected through polymerase chain reaction (PCR). Results The results showed that none of the 22 microbiota isolates carried class 1 integrons. Among the 29 clinical isolates, 19 (65.5%) contained class 1 integrons, and resistance to sulfamethoxazole/trimethoprim was identified in 18 of these isolates (94.7%). Among the K. pneumoniae isolates with class 1 integrons, 47% belonged to the KpI phylogenetic group, and one isolate (14.3%) carrying these genetic elements belonged to the KpIII group. Conclusions The wide variety of detected class 1 integrons supports the presence of high rates of antimicrobial resistance, genetic variability, and rapid dissemination of beta-lactamase genes among K. pneumoniae clinical isolates in recent years in hospitals in Recife-PE, Brazil. The findings of this study indicate that the surveillance of K. pneumoniae integrons in clinical isolates could be useful for monitoring the spread of antibiotic resistance genes in the hospital environment. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 47 2 165 169 |
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Class 1 integron Klebsiella pneumonia Phylogenetic groups Arctic medicine. Tropical medicine RC955-962 |
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Class 1 integron Klebsiella pneumonia Phylogenetic groups Arctic medicine. Tropical medicine RC955-962 Alexsandra Maria Silva Lima Maíra Espíndola Silva de Melo Luiz Carlos Alves Fábio André Brayner Ana Catarina Souza Lopes Investigation of class 1 integrons in Klebsiella pneumoniae clinical and microbiota isolates belonging to different phylogenetic groups in Recife, State of Pernambuco |
topic_facet |
Class 1 integron Klebsiella pneumonia Phylogenetic groups Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction The high prevalence of Klebsiella pneumoniae infections is related to the ability of K. pneumoniae to acquire and disseminate exogenous genes associated with mobile elements, such as R plasmids, transposons and integrons. This study investigated the presence of class 1 integrons in clinical and microbiota isolates of K. pneumoniae belonging to different phylogenetic groups and correlated these results with the antimicrobial resistance profiles of the studied isolates. Methods Of the 51 isolates of K. pneumoniae selected for this study, 29 were from multidrug-resistant clinical isolates, and 22 were from children's microbiota. The susceptibility profile was determined using the disk diffusion method, and class 1 integrons were detected through polymerase chain reaction (PCR). Results The results showed that none of the 22 microbiota isolates carried class 1 integrons. Among the 29 clinical isolates, 19 (65.5%) contained class 1 integrons, and resistance to sulfamethoxazole/trimethoprim was identified in 18 of these isolates (94.7%). Among the K. pneumoniae isolates with class 1 integrons, 47% belonged to the KpI phylogenetic group, and one isolate (14.3%) carrying these genetic elements belonged to the KpIII group. Conclusions The wide variety of detected class 1 integrons supports the presence of high rates of antimicrobial resistance, genetic variability, and rapid dissemination of beta-lactamase genes among K. pneumoniae clinical isolates in recent years in hospitals in Recife-PE, Brazil. The findings of this study indicate that the surveillance of K. pneumoniae integrons in clinical isolates could be useful for monitoring the spread of antibiotic resistance genes in the hospital environment. |
format |
Article in Journal/Newspaper |
author |
Alexsandra Maria Silva Lima Maíra Espíndola Silva de Melo Luiz Carlos Alves Fábio André Brayner Ana Catarina Souza Lopes |
author_facet |
Alexsandra Maria Silva Lima Maíra Espíndola Silva de Melo Luiz Carlos Alves Fábio André Brayner Ana Catarina Souza Lopes |
author_sort |
Alexsandra Maria Silva Lima |
title |
Investigation of class 1 integrons in Klebsiella pneumoniae clinical and microbiota isolates belonging to different phylogenetic groups in Recife, State of Pernambuco |
title_short |
Investigation of class 1 integrons in Klebsiella pneumoniae clinical and microbiota isolates belonging to different phylogenetic groups in Recife, State of Pernambuco |
title_full |
Investigation of class 1 integrons in Klebsiella pneumoniae clinical and microbiota isolates belonging to different phylogenetic groups in Recife, State of Pernambuco |
title_fullStr |
Investigation of class 1 integrons in Klebsiella pneumoniae clinical and microbiota isolates belonging to different phylogenetic groups in Recife, State of Pernambuco |
title_full_unstemmed |
Investigation of class 1 integrons in Klebsiella pneumoniae clinical and microbiota isolates belonging to different phylogenetic groups in Recife, State of Pernambuco |
title_sort |
investigation of class 1 integrons in klebsiella pneumoniae clinical and microbiota isolates belonging to different phylogenetic groups in recife, state of pernambuco |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2014 |
url |
https://doi.org/10.1590/0037-8682-0021-2014 https://doaj.org/article/015356d079d54308acb6100d1cbe48d6 |
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Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 47, Iss 2, Pp 165-169 (2014) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000200165&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0021-2014 https://doaj.org/article/015356d079d54308acb6100d1cbe48d6 |
op_doi |
https://doi.org/10.1590/0037-8682-0021-2014 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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47 |
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165 |
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