Antimicrobial susceptibilities of Listeria monocytogenes human strains isolated from 1970 to 2008 in Brazil
INTRODUCTION: Listeria monocytogenes is the causative agent of listeriosis, a foodborne illness that affects mainly pregnant women, the elderly and immunocompromised patients. The primary treatment is a combination of ampicillin with an aminoglycoside, in addition to a second-choice drug represented...
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ftdoajarticles:oai:doaj.org/article:014a4078a5e14be9b1fd77375580e1e1 2023-05-15T15:10:56+02:00 Antimicrobial susceptibilities of Listeria monocytogenes human strains isolated from 1970 to 2008 in Brazil Cristhiane Moura Falavina dos Reis André Victor Barbosa Leonardo Alves Rusak Deyse Christina Vallim Ernesto Hofer 2011-04-01T00:00:00Z https://doi.org/10.1590/s0037-86822011005000019 https://doaj.org/article/014a4078a5e14be9b1fd77375580e1e1 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000200009&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/s0037-86822011005000019 https://doaj.org/article/014a4078a5e14be9b1fd77375580e1e1 Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 2, Pp 173-176 (2011) Listeria monocytogenes Susceptibilidade antimicrobiana Listeriose Arctic medicine. Tropical medicine RC955-962 article 2011 ftdoajarticles https://doi.org/10.1590/s0037-86822011005000019 2022-12-31T02:55:15Z INTRODUCTION: Listeria monocytogenes is the causative agent of listeriosis, a foodborne illness that affects mainly pregnant women, the elderly and immunocompromised patients. The primary treatment is a combination of ampicillin with an aminoglycoside, in addition to a second-choice drug represented by chloramphenicol, erythromycin, tetracycline and rifampicin. The aim of this study was to analyze the antimicrobial susceptibility profile of strains isolated from human sources in the last four decades. METHODS: Sixty-eight strains were selected from the culture collection of the Laboratory of Bacterial Zoonoses/LABZOO/FIOCRUZ isolated in different regions of Brazil from 1970 to 2008 and primarily isolated from cerebrospinal fluid and blood culture. Susceptibility tests to antimicrobials drugs were evaluated using the criteria established by Soussy using the Kirby-Bauer method and E-Test strips were used to determine the minimum inhibitory concentration (MIC). RESULTS: Among the strains tested, serovar L4b (60.3%) was the most prevalent, followed by serovar 1/2a (20.6%), 1/2b (13.2%) and the more uncommon serovars 1/2c, 3b and 4ab (5.9%). All strains were susceptible to ampicillin, cephalothin, erythromycin, gentamicin, teicoplanin and vancomycin. Only one strain (1.5%) showed resistance to rifampin, and two (3%) were resistant to trimethoprim-sulfamethoxazole. MICs with values up to 2μg/ml reinforce the need for microbiological surveillance. CONCLUSIONS: The study demonstrated low prevalence of strains resistant to the antimicrobial drugs indicated in the treatment of human listeriosis. Monitoring antimicrobial resistance profile is still very important to determine adequate treatment, especially in immunocompromised patients. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 44 2 173 176 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Listeria monocytogenes Susceptibilidade antimicrobiana Listeriose Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Listeria monocytogenes Susceptibilidade antimicrobiana Listeriose Arctic medicine. Tropical medicine RC955-962 Cristhiane Moura Falavina dos Reis André Victor Barbosa Leonardo Alves Rusak Deyse Christina Vallim Ernesto Hofer Antimicrobial susceptibilities of Listeria monocytogenes human strains isolated from 1970 to 2008 in Brazil |
topic_facet |
Listeria monocytogenes Susceptibilidade antimicrobiana Listeriose Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: Listeria monocytogenes is the causative agent of listeriosis, a foodborne illness that affects mainly pregnant women, the elderly and immunocompromised patients. The primary treatment is a combination of ampicillin with an aminoglycoside, in addition to a second-choice drug represented by chloramphenicol, erythromycin, tetracycline and rifampicin. The aim of this study was to analyze the antimicrobial susceptibility profile of strains isolated from human sources in the last four decades. METHODS: Sixty-eight strains were selected from the culture collection of the Laboratory of Bacterial Zoonoses/LABZOO/FIOCRUZ isolated in different regions of Brazil from 1970 to 2008 and primarily isolated from cerebrospinal fluid and blood culture. Susceptibility tests to antimicrobials drugs were evaluated using the criteria established by Soussy using the Kirby-Bauer method and E-Test strips were used to determine the minimum inhibitory concentration (MIC). RESULTS: Among the strains tested, serovar L4b (60.3%) was the most prevalent, followed by serovar 1/2a (20.6%), 1/2b (13.2%) and the more uncommon serovars 1/2c, 3b and 4ab (5.9%). All strains were susceptible to ampicillin, cephalothin, erythromycin, gentamicin, teicoplanin and vancomycin. Only one strain (1.5%) showed resistance to rifampin, and two (3%) were resistant to trimethoprim-sulfamethoxazole. MICs with values up to 2μg/ml reinforce the need for microbiological surveillance. CONCLUSIONS: The study demonstrated low prevalence of strains resistant to the antimicrobial drugs indicated in the treatment of human listeriosis. Monitoring antimicrobial resistance profile is still very important to determine adequate treatment, especially in immunocompromised patients. |
format |
Article in Journal/Newspaper |
author |
Cristhiane Moura Falavina dos Reis André Victor Barbosa Leonardo Alves Rusak Deyse Christina Vallim Ernesto Hofer |
author_facet |
Cristhiane Moura Falavina dos Reis André Victor Barbosa Leonardo Alves Rusak Deyse Christina Vallim Ernesto Hofer |
author_sort |
Cristhiane Moura Falavina dos Reis |
title |
Antimicrobial susceptibilities of Listeria monocytogenes human strains isolated from 1970 to 2008 in Brazil |
title_short |
Antimicrobial susceptibilities of Listeria monocytogenes human strains isolated from 1970 to 2008 in Brazil |
title_full |
Antimicrobial susceptibilities of Listeria monocytogenes human strains isolated from 1970 to 2008 in Brazil |
title_fullStr |
Antimicrobial susceptibilities of Listeria monocytogenes human strains isolated from 1970 to 2008 in Brazil |
title_full_unstemmed |
Antimicrobial susceptibilities of Listeria monocytogenes human strains isolated from 1970 to 2008 in Brazil |
title_sort |
antimicrobial susceptibilities of listeria monocytogenes human strains isolated from 1970 to 2008 in brazil |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2011 |
url |
https://doi.org/10.1590/s0037-86822011005000019 https://doaj.org/article/014a4078a5e14be9b1fd77375580e1e1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 2, Pp 173-176 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000200009&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/s0037-86822011005000019 https://doaj.org/article/014a4078a5e14be9b1fd77375580e1e1 |
op_doi |
https://doi.org/10.1590/s0037-86822011005000019 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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44 |
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173 |
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176 |
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