Identification of Burkholderia pseudomallei Near-Neighbor Species in the Northern Territory of Australia.
Identification and characterization of near-neighbor species are critical to the development of robust molecular diagnostic tools for biothreat agents. One such agent, Burkholderia pseudomallei, a soil bacterium and the causative agent of melioidosis, is lacking in this area because of its genomic d...
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ftdoajarticles:oai:doaj.org/article:013772fc89b34e5a89ca2e68c2adb14c 2023-05-15T15:11:41+02:00 Identification of Burkholderia pseudomallei Near-Neighbor Species in the Northern Territory of Australia. Jennifer L Ginther Mark Mayo Stephanie D Warrington Mirjam Kaestli Travis Mullins David M Wagner Bart J Currie Apichai Tuanyok Paul Keim 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003892 https://doaj.org/article/013772fc89b34e5a89ca2e68c2adb14c EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4486726?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003892 https://doaj.org/article/013772fc89b34e5a89ca2e68c2adb14c PLoS Neglected Tropical Diseases, Vol 9, Iss 6, p e0003892 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003892 2022-12-31T03:12:05Z Identification and characterization of near-neighbor species are critical to the development of robust molecular diagnostic tools for biothreat agents. One such agent, Burkholderia pseudomallei, a soil bacterium and the causative agent of melioidosis, is lacking in this area because of its genomic diversity and widespread geographic distribution. The Burkholderia genus contains over 60 species and occupies a large range of environments including soil, plants, rhizospheres, water, animals and humans. The identification of novel species in new locations necessitates the need to identify the true global distribution of Burkholderia species, especially the members that are closely related to B. pseudomallei. In our current study, we used the Burkholderia-specific recA sequencing assay to analyze environmental samples from the Darwin region in the Northern Territory of Australia where melioidosis is endemic. Burkholderia recA PCR negative samples were further characterized using 16s rRNA sequencing for species identification. Phylogenetic analysis demonstrated that over 70% of the bacterial isolates were identified as B. ubonensis indicating that this species is common in the soil where B. pseudomallei is endemic. Bayesian phylogenetic analysis reveals many novel branches within the B. cepacia complex, one novel B. oklahomensis-like species, and one novel branch containing one isolate that is distinct from all other samples on the phylogenetic tree. During the analysis with recA sequencing, we discovered 2 single nucleotide polymorphisms in the reverse priming region of B. oklahomensis. A degenerate primer was developed and is proposed for future use. We conclude that the recA sequencing technique is an effective tool to classify Burkholderia and identify soil organisms in a melioidosis endemic area. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 6 e0003892 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Jennifer L Ginther Mark Mayo Stephanie D Warrington Mirjam Kaestli Travis Mullins David M Wagner Bart J Currie Apichai Tuanyok Paul Keim Identification of Burkholderia pseudomallei Near-Neighbor Species in the Northern Territory of Australia. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Identification and characterization of near-neighbor species are critical to the development of robust molecular diagnostic tools for biothreat agents. One such agent, Burkholderia pseudomallei, a soil bacterium and the causative agent of melioidosis, is lacking in this area because of its genomic diversity and widespread geographic distribution. The Burkholderia genus contains over 60 species and occupies a large range of environments including soil, plants, rhizospheres, water, animals and humans. The identification of novel species in new locations necessitates the need to identify the true global distribution of Burkholderia species, especially the members that are closely related to B. pseudomallei. In our current study, we used the Burkholderia-specific recA sequencing assay to analyze environmental samples from the Darwin region in the Northern Territory of Australia where melioidosis is endemic. Burkholderia recA PCR negative samples were further characterized using 16s rRNA sequencing for species identification. Phylogenetic analysis demonstrated that over 70% of the bacterial isolates were identified as B. ubonensis indicating that this species is common in the soil where B. pseudomallei is endemic. Bayesian phylogenetic analysis reveals many novel branches within the B. cepacia complex, one novel B. oklahomensis-like species, and one novel branch containing one isolate that is distinct from all other samples on the phylogenetic tree. During the analysis with recA sequencing, we discovered 2 single nucleotide polymorphisms in the reverse priming region of B. oklahomensis. A degenerate primer was developed and is proposed for future use. We conclude that the recA sequencing technique is an effective tool to classify Burkholderia and identify soil organisms in a melioidosis endemic area. |
format |
Article in Journal/Newspaper |
author |
Jennifer L Ginther Mark Mayo Stephanie D Warrington Mirjam Kaestli Travis Mullins David M Wagner Bart J Currie Apichai Tuanyok Paul Keim |
author_facet |
Jennifer L Ginther Mark Mayo Stephanie D Warrington Mirjam Kaestli Travis Mullins David M Wagner Bart J Currie Apichai Tuanyok Paul Keim |
author_sort |
Jennifer L Ginther |
title |
Identification of Burkholderia pseudomallei Near-Neighbor Species in the Northern Territory of Australia. |
title_short |
Identification of Burkholderia pseudomallei Near-Neighbor Species in the Northern Territory of Australia. |
title_full |
Identification of Burkholderia pseudomallei Near-Neighbor Species in the Northern Territory of Australia. |
title_fullStr |
Identification of Burkholderia pseudomallei Near-Neighbor Species in the Northern Territory of Australia. |
title_full_unstemmed |
Identification of Burkholderia pseudomallei Near-Neighbor Species in the Northern Territory of Australia. |
title_sort |
identification of burkholderia pseudomallei near-neighbor species in the northern territory of australia. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0003892 https://doaj.org/article/013772fc89b34e5a89ca2e68c2adb14c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 6, p e0003892 (2015) |
op_relation |
http://europepmc.org/articles/PMC4486726?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003892 https://doaj.org/article/013772fc89b34e5a89ca2e68c2adb14c |
op_doi |
https://doi.org/10.1371/journal.pntd.0003892 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
9 |
container_issue |
6 |
container_start_page |
e0003892 |
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1766342504459796480 |