Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements

Menaquinone (MK) has an important role in human metabolism as an essential vitamin (VK2), which is mainly produced through the fermentation of microorganisms. MK8(H2) was identified to be the main menaquinone from Rhodococcus sp. B7740, a bacterium isolated from the arctic ocean. In this work, MK8(H...

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Published in:Marine Drugs
Main Authors: Yashu Chen, Qin Mu, Kai Hu, Mo Chen, Jifang Yang, Jigang Chen, Bijun Xie, Zhida Sun
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2018
Subjects:
NMR
Online Access:https://doi.org/10.3390/md16100391
https://doaj.org/article/01180d90f1544303aa662a0ace4080a5
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spelling ftdoajarticles:oai:doaj.org/article:01180d90f1544303aa662a0ace4080a5 2023-05-15T14:56:06+02:00 Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements Yashu Chen Qin Mu Kai Hu Mo Chen Jifang Yang Jigang Chen Bijun Xie Zhida Sun 2018-10-01T00:00:00Z https://doi.org/10.3390/md16100391 https://doaj.org/article/01180d90f1544303aa662a0ace4080a5 EN eng MDPI AG http://www.mdpi.com/1660-3397/16/10/391 https://doaj.org/toc/1660-3397 1660-3397 doi:10.3390/md16100391 https://doaj.org/article/01180d90f1544303aa662a0ace4080a5 Marine Drugs, Vol 16, Iss 10, p 391 (2018) isoprenoid quinone MK8(H2) Rhodococcus sp. B7740 from Arctic ocean UPLC-HRMS NMR antioxidant and antiglycation Biology (General) QH301-705.5 article 2018 ftdoajarticles https://doi.org/10.3390/md16100391 2022-12-30T22:31:32Z Menaquinone (MK) has an important role in human metabolism as an essential vitamin (VK2), which is mainly produced through the fermentation of microorganisms. MK8(H2) was identified to be the main menaquinone from Rhodococcus sp. B7740, a bacterium isolated from the arctic ocean. In this work, MK8(H2) (purity: 99.75%) was collected through a convenient and economic extraction process followed by high-speed countercurrent chromatography (HSCCC) purification. Additionally, high-resolution mass spectrometry (HRMS) was performed for further identification and the hydrogenation position of MK8(H2) (terminal unit) was determined using nuclear magnetic resonance (NMR) for the first time. MK8(H2) showed a superior antioxidant effect and antiglycation capacity compared with ubiquinone Q10 and MK4. High-performance liquid chromatography–mass spectrometer (HPLC-MS/MS) and molecular docking showed the fine interaction between MK8(H2) with methylglyoxal (MGO) and bull serum albumin (BSA), respectively. These properties make MK8(H2) a promising natural active ingredient with future food and medicine applications. Article in Journal/Newspaper Arctic Arctic Ocean Directory of Open Access Journals: DOAJ Articles Arctic Arctic Ocean Marine Drugs 16 10 391
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic isoprenoid quinone
MK8(H2)
Rhodococcus sp. B7740 from Arctic ocean
UPLC-HRMS
NMR
antioxidant and antiglycation
Biology (General)
QH301-705.5
spellingShingle isoprenoid quinone
MK8(H2)
Rhodococcus sp. B7740 from Arctic ocean
UPLC-HRMS
NMR
antioxidant and antiglycation
Biology (General)
QH301-705.5
Yashu Chen
Qin Mu
Kai Hu
Mo Chen
Jifang Yang
Jigang Chen
Bijun Xie
Zhida Sun
Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements
topic_facet isoprenoid quinone
MK8(H2)
Rhodococcus sp. B7740 from Arctic ocean
UPLC-HRMS
NMR
antioxidant and antiglycation
Biology (General)
QH301-705.5
description Menaquinone (MK) has an important role in human metabolism as an essential vitamin (VK2), which is mainly produced through the fermentation of microorganisms. MK8(H2) was identified to be the main menaquinone from Rhodococcus sp. B7740, a bacterium isolated from the arctic ocean. In this work, MK8(H2) (purity: 99.75%) was collected through a convenient and economic extraction process followed by high-speed countercurrent chromatography (HSCCC) purification. Additionally, high-resolution mass spectrometry (HRMS) was performed for further identification and the hydrogenation position of MK8(H2) (terminal unit) was determined using nuclear magnetic resonance (NMR) for the first time. MK8(H2) showed a superior antioxidant effect and antiglycation capacity compared with ubiquinone Q10 and MK4. High-performance liquid chromatography–mass spectrometer (HPLC-MS/MS) and molecular docking showed the fine interaction between MK8(H2) with methylglyoxal (MGO) and bull serum albumin (BSA), respectively. These properties make MK8(H2) a promising natural active ingredient with future food and medicine applications.
format Article in Journal/Newspaper
author Yashu Chen
Qin Mu
Kai Hu
Mo Chen
Jifang Yang
Jigang Chen
Bijun Xie
Zhida Sun
author_facet Yashu Chen
Qin Mu
Kai Hu
Mo Chen
Jifang Yang
Jigang Chen
Bijun Xie
Zhida Sun
author_sort Yashu Chen
title Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements
title_short Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements
title_full Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements
title_fullStr Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements
title_full_unstemmed Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements
title_sort characterization of mk8(h2) from rhodococcus sp. b7740 and its potential antiglycation capacity measurements
publisher MDPI AG
publishDate 2018
url https://doi.org/10.3390/md16100391
https://doaj.org/article/01180d90f1544303aa662a0ace4080a5
geographic Arctic
Arctic Ocean
geographic_facet Arctic
Arctic Ocean
genre Arctic
Arctic Ocean
genre_facet Arctic
Arctic Ocean
op_source Marine Drugs, Vol 16, Iss 10, p 391 (2018)
op_relation http://www.mdpi.com/1660-3397/16/10/391
https://doaj.org/toc/1660-3397
1660-3397
doi:10.3390/md16100391
https://doaj.org/article/01180d90f1544303aa662a0ace4080a5
op_doi https://doi.org/10.3390/md16100391
container_title Marine Drugs
container_volume 16
container_issue 10
container_start_page 391
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