Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements
Menaquinone (MK) has an important role in human metabolism as an essential vitamin (VK2), which is mainly produced through the fermentation of microorganisms. MK8(H2) was identified to be the main menaquinone from Rhodococcus sp. B7740, a bacterium isolated from the arctic ocean. In this work, MK8(H...
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ftdoajarticles:oai:doaj.org/article:01180d90f1544303aa662a0ace4080a5 2023-05-15T14:56:06+02:00 Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements Yashu Chen Qin Mu Kai Hu Mo Chen Jifang Yang Jigang Chen Bijun Xie Zhida Sun 2018-10-01T00:00:00Z https://doi.org/10.3390/md16100391 https://doaj.org/article/01180d90f1544303aa662a0ace4080a5 EN eng MDPI AG http://www.mdpi.com/1660-3397/16/10/391 https://doaj.org/toc/1660-3397 1660-3397 doi:10.3390/md16100391 https://doaj.org/article/01180d90f1544303aa662a0ace4080a5 Marine Drugs, Vol 16, Iss 10, p 391 (2018) isoprenoid quinone MK8(H2) Rhodococcus sp. B7740 from Arctic ocean UPLC-HRMS NMR antioxidant and antiglycation Biology (General) QH301-705.5 article 2018 ftdoajarticles https://doi.org/10.3390/md16100391 2022-12-30T22:31:32Z Menaquinone (MK) has an important role in human metabolism as an essential vitamin (VK2), which is mainly produced through the fermentation of microorganisms. MK8(H2) was identified to be the main menaquinone from Rhodococcus sp. B7740, a bacterium isolated from the arctic ocean. In this work, MK8(H2) (purity: 99.75%) was collected through a convenient and economic extraction process followed by high-speed countercurrent chromatography (HSCCC) purification. Additionally, high-resolution mass spectrometry (HRMS) was performed for further identification and the hydrogenation position of MK8(H2) (terminal unit) was determined using nuclear magnetic resonance (NMR) for the first time. MK8(H2) showed a superior antioxidant effect and antiglycation capacity compared with ubiquinone Q10 and MK4. High-performance liquid chromatography–mass spectrometer (HPLC-MS/MS) and molecular docking showed the fine interaction between MK8(H2) with methylglyoxal (MGO) and bull serum albumin (BSA), respectively. These properties make MK8(H2) a promising natural active ingredient with future food and medicine applications. Article in Journal/Newspaper Arctic Arctic Ocean Directory of Open Access Journals: DOAJ Articles Arctic Arctic Ocean Marine Drugs 16 10 391 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
isoprenoid quinone MK8(H2) Rhodococcus sp. B7740 from Arctic ocean UPLC-HRMS NMR antioxidant and antiglycation Biology (General) QH301-705.5 |
spellingShingle |
isoprenoid quinone MK8(H2) Rhodococcus sp. B7740 from Arctic ocean UPLC-HRMS NMR antioxidant and antiglycation Biology (General) QH301-705.5 Yashu Chen Qin Mu Kai Hu Mo Chen Jifang Yang Jigang Chen Bijun Xie Zhida Sun Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements |
topic_facet |
isoprenoid quinone MK8(H2) Rhodococcus sp. B7740 from Arctic ocean UPLC-HRMS NMR antioxidant and antiglycation Biology (General) QH301-705.5 |
description |
Menaquinone (MK) has an important role in human metabolism as an essential vitamin (VK2), which is mainly produced through the fermentation of microorganisms. MK8(H2) was identified to be the main menaquinone from Rhodococcus sp. B7740, a bacterium isolated from the arctic ocean. In this work, MK8(H2) (purity: 99.75%) was collected through a convenient and economic extraction process followed by high-speed countercurrent chromatography (HSCCC) purification. Additionally, high-resolution mass spectrometry (HRMS) was performed for further identification and the hydrogenation position of MK8(H2) (terminal unit) was determined using nuclear magnetic resonance (NMR) for the first time. MK8(H2) showed a superior antioxidant effect and antiglycation capacity compared with ubiquinone Q10 and MK4. High-performance liquid chromatography–mass spectrometer (HPLC-MS/MS) and molecular docking showed the fine interaction between MK8(H2) with methylglyoxal (MGO) and bull serum albumin (BSA), respectively. These properties make MK8(H2) a promising natural active ingredient with future food and medicine applications. |
format |
Article in Journal/Newspaper |
author |
Yashu Chen Qin Mu Kai Hu Mo Chen Jifang Yang Jigang Chen Bijun Xie Zhida Sun |
author_facet |
Yashu Chen Qin Mu Kai Hu Mo Chen Jifang Yang Jigang Chen Bijun Xie Zhida Sun |
author_sort |
Yashu Chen |
title |
Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements |
title_short |
Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements |
title_full |
Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements |
title_fullStr |
Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements |
title_full_unstemmed |
Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements |
title_sort |
characterization of mk8(h2) from rhodococcus sp. b7740 and its potential antiglycation capacity measurements |
publisher |
MDPI AG |
publishDate |
2018 |
url |
https://doi.org/10.3390/md16100391 https://doaj.org/article/01180d90f1544303aa662a0ace4080a5 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean |
genre_facet |
Arctic Arctic Ocean |
op_source |
Marine Drugs, Vol 16, Iss 10, p 391 (2018) |
op_relation |
http://www.mdpi.com/1660-3397/16/10/391 https://doaj.org/toc/1660-3397 1660-3397 doi:10.3390/md16100391 https://doaj.org/article/01180d90f1544303aa662a0ace4080a5 |
op_doi |
https://doi.org/10.3390/md16100391 |
container_title |
Marine Drugs |
container_volume |
16 |
container_issue |
10 |
container_start_page |
391 |
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1766328139630247936 |