Thai Perilla frutescens fruit oil alleviates carbon tetrachloride-induced hepatotoxicities in rats
Objective: To study the effect of perilla fruit oil against carbon tetrachloride (CCl4)-induced liver damage in rats. Methods: Perilla fruit oil was analyzed in terms of fatty acids, tocopherols and tocotrienols using chromatography. Sub-chronic toxicity of perilla fruit oil was investigated in rats...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2020
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Online Access: | https://doi.org/10.4103/2221-1691.276316 https://doaj.org/article/00d73c85b35a4c47b085775815130e21 |
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ftdoajarticles:oai:doaj.org/article:00d73c85b35a4c47b085775815130e21 2023-05-15T15:16:27+02:00 Thai Perilla frutescens fruit oil alleviates carbon tetrachloride-induced hepatotoxicities in rats Narisara Paradee Duangta Kanjanapothi Tawat Taesotikul Sarawut Kongkarnka Adchara Prommaban Pimpisid Koonyosying Somdet Srichairatanakool 2020-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.276316 https://doaj.org/article/00d73c85b35a4c47b085775815130e21 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=3;spage=101;epage=110;aulast=Paradee https://doaj.org/toc/2588-9222 2588-9222 doi:10.4103/2221-1691.276316 https://doaj.org/article/00d73c85b35a4c47b085775815130e21 Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 3, Pp 101-110 (2020) perilla frutescens perilla oil carbon tetrachloride fatty change liver injury Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.4103/2221-1691.276316 2022-12-31T12:11:06Z Objective: To study the effect of perilla fruit oil against carbon tetrachloride (CCl4)-induced liver damage in rats. Methods: Perilla fruit oil was analyzed in terms of fatty acids, tocopherols and tocotrienols using chromatography. Sub-chronic toxicity of perilla fruit oil was investigated in rats for 90 d followed by a 28 d recovery period. Hematological, biochemical and pathological parameters were determined. To evaluate hepatoprotection, rats were divided into five groups and orally administered with Tween 80 for 10 d; Tween 80, silymarin, perilla fruit oil (0.1 mL/200 g) and perilla fruit oil (1 mL/200 g) for 10 d together with subcutaneous injection of CCl4 (2 mL/200 g) on days 9 and 10. Liver enzymes and pathological parameters were determined. Results: Perilla fruit oil contained α-linolenic acid (56.55% of total fatty acid), β-tocopherol (49.50 mg/kg) and γ-tocotrienol (43.65 mg/ kg). Rats showed significant changes in the percentage of monocytes and platelet indices following perilla fruit oil consumption for 90 d; in the percentage of neutrophils and lymphocytes, and RBC indices in the recovery period when compared with the deionized water group. Total protein and creatinine levels were increased while alkaline phosphatase and aspartate aminotransferase levels were decreased (P < 0.05). Organ weight index and pathological indicators did not change significantly. The liver of CCl4-induced rats showed remarkable centrilobular fatty changes, which was ameliorated by perilla fruit oil pretreatment. Aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase levels were decreased (P < 0.05) in rats given perilla fruit oil. Conclusions: Perilla fruit oil is rich in α-linolenic acid, β-tocopherol and γ-tocotrienol and improves blood biomarker levels and protects against CCl4-induced hepatotoxicity. Further studies are required before supporting its use for the treatment of hepatitis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 10 3 101 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
perilla frutescens perilla oil carbon tetrachloride fatty change liver injury Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
perilla frutescens perilla oil carbon tetrachloride fatty change liver injury Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Narisara Paradee Duangta Kanjanapothi Tawat Taesotikul Sarawut Kongkarnka Adchara Prommaban Pimpisid Koonyosying Somdet Srichairatanakool Thai Perilla frutescens fruit oil alleviates carbon tetrachloride-induced hepatotoxicities in rats |
topic_facet |
perilla frutescens perilla oil carbon tetrachloride fatty change liver injury Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To study the effect of perilla fruit oil against carbon tetrachloride (CCl4)-induced liver damage in rats. Methods: Perilla fruit oil was analyzed in terms of fatty acids, tocopherols and tocotrienols using chromatography. Sub-chronic toxicity of perilla fruit oil was investigated in rats for 90 d followed by a 28 d recovery period. Hematological, biochemical and pathological parameters were determined. To evaluate hepatoprotection, rats were divided into five groups and orally administered with Tween 80 for 10 d; Tween 80, silymarin, perilla fruit oil (0.1 mL/200 g) and perilla fruit oil (1 mL/200 g) for 10 d together with subcutaneous injection of CCl4 (2 mL/200 g) on days 9 and 10. Liver enzymes and pathological parameters were determined. Results: Perilla fruit oil contained α-linolenic acid (56.55% of total fatty acid), β-tocopherol (49.50 mg/kg) and γ-tocotrienol (43.65 mg/ kg). Rats showed significant changes in the percentage of monocytes and platelet indices following perilla fruit oil consumption for 90 d; in the percentage of neutrophils and lymphocytes, and RBC indices in the recovery period when compared with the deionized water group. Total protein and creatinine levels were increased while alkaline phosphatase and aspartate aminotransferase levels were decreased (P < 0.05). Organ weight index and pathological indicators did not change significantly. The liver of CCl4-induced rats showed remarkable centrilobular fatty changes, which was ameliorated by perilla fruit oil pretreatment. Aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase levels were decreased (P < 0.05) in rats given perilla fruit oil. Conclusions: Perilla fruit oil is rich in α-linolenic acid, β-tocopherol and γ-tocotrienol and improves blood biomarker levels and protects against CCl4-induced hepatotoxicity. Further studies are required before supporting its use for the treatment of hepatitis. |
format |
Article in Journal/Newspaper |
author |
Narisara Paradee Duangta Kanjanapothi Tawat Taesotikul Sarawut Kongkarnka Adchara Prommaban Pimpisid Koonyosying Somdet Srichairatanakool |
author_facet |
Narisara Paradee Duangta Kanjanapothi Tawat Taesotikul Sarawut Kongkarnka Adchara Prommaban Pimpisid Koonyosying Somdet Srichairatanakool |
author_sort |
Narisara Paradee |
title |
Thai Perilla frutescens fruit oil alleviates carbon tetrachloride-induced hepatotoxicities in rats |
title_short |
Thai Perilla frutescens fruit oil alleviates carbon tetrachloride-induced hepatotoxicities in rats |
title_full |
Thai Perilla frutescens fruit oil alleviates carbon tetrachloride-induced hepatotoxicities in rats |
title_fullStr |
Thai Perilla frutescens fruit oil alleviates carbon tetrachloride-induced hepatotoxicities in rats |
title_full_unstemmed |
Thai Perilla frutescens fruit oil alleviates carbon tetrachloride-induced hepatotoxicities in rats |
title_sort |
thai perilla frutescens fruit oil alleviates carbon tetrachloride-induced hepatotoxicities in rats |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2020 |
url |
https://doi.org/10.4103/2221-1691.276316 https://doaj.org/article/00d73c85b35a4c47b085775815130e21 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 3, Pp 101-110 (2020) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=3;spage=101;epage=110;aulast=Paradee https://doaj.org/toc/2588-9222 2588-9222 doi:10.4103/2221-1691.276316 https://doaj.org/article/00d73c85b35a4c47b085775815130e21 |
op_doi |
https://doi.org/10.4103/2221-1691.276316 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
10 |
container_issue |
3 |
container_start_page |
101 |
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1766346749185622016 |