Clonal structure of Trypanosoma cruzi Colombian strain (biodeme Type III): biological, isoenzymic and histopathological analysis of seven isolated clones
The clonal structure of the Colombian strain of Trypanosoma cruzi, biodeme Type III and zymodeme 1, was analyzed in order to characterize its populations and to establish its homogeneity or heterogeneity. Seven isolated clones presented the basic characteristics of Biodeme Type III, with the same pa...
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Sociedade Brasileira de Medicina Tropical (SBMT)
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ftdoajarticles:oai:doaj.org/article:00c742bd7afb44a59008f615d38358e6 2023-05-15T15:06:40+02:00 Clonal structure of Trypanosoma cruzi Colombian strain (biodeme Type III): biological, isoenzymic and histopathological analysis of seven isolated clones Camandaroba Edson Luiz Paes Campos Rozália Figueira Magalhães Juracy Barbosa Andrade Sonia G. 2001-01-01T00:00:00Z https://doaj.org/article/00c742bd7afb44a59008f615d38358e6 EN ES PT eng spa por Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822001000200001 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/00c742bd7afb44a59008f615d38358e6 Revista da Sociedade Brasileira de Medicina Tropical, Vol 34, Iss 2, Pp 151-157 (2001) Trypanosoma cruzi Colombian strain Clonal structure Clones Biodemes Zymodemes. Histopathology Arctic medicine. Tropical medicine RC955-962 article 2001 ftdoajarticles 2023-01-08T01:29:35Z The clonal structure of the Colombian strain of Trypanosoma cruzi, biodeme Type III and zymodeme 1, was analyzed in order to characterize its populations and to establish its homogeneity or heterogeneity. Seven isolated clones presented the basic characteristics of Biodeme Type III, with the same patterns of parasitemic curves, tissue tropism to skeletal muscle and myocardium, high pathogenicity with extensive necrotic-inflammatory lesions from the 20th to 30th day of infection. The parental strain and its clones C1, C3, C4 and C6, determined the higher levels of parasitemia, 20 to 30 days of infection, with high mortality rate up to 30 days (79 to 100%); clones C2, C5 and C7 presented lower levels of parasitemia, with low mortality rates (7.6 to 23%). Isoenzymic patterns, characteristic of zymodeme 1, (Z1) were similar for the parental strain and its seven clones. Results point to a phenotypic homogeneity of the clones isolated from the Colombian strain and suggest the predominance of a principal clone, responsible for the biological behavior of the parental strain and clones. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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English Spanish Portuguese |
topic |
Trypanosoma cruzi Colombian strain Clonal structure Clones Biodemes Zymodemes. Histopathology Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Trypanosoma cruzi Colombian strain Clonal structure Clones Biodemes Zymodemes. Histopathology Arctic medicine. Tropical medicine RC955-962 Camandaroba Edson Luiz Paes Campos Rozália Figueira Magalhães Juracy Barbosa Andrade Sonia G. Clonal structure of Trypanosoma cruzi Colombian strain (biodeme Type III): biological, isoenzymic and histopathological analysis of seven isolated clones |
topic_facet |
Trypanosoma cruzi Colombian strain Clonal structure Clones Biodemes Zymodemes. Histopathology Arctic medicine. Tropical medicine RC955-962 |
description |
The clonal structure of the Colombian strain of Trypanosoma cruzi, biodeme Type III and zymodeme 1, was analyzed in order to characterize its populations and to establish its homogeneity or heterogeneity. Seven isolated clones presented the basic characteristics of Biodeme Type III, with the same patterns of parasitemic curves, tissue tropism to skeletal muscle and myocardium, high pathogenicity with extensive necrotic-inflammatory lesions from the 20th to 30th day of infection. The parental strain and its clones C1, C3, C4 and C6, determined the higher levels of parasitemia, 20 to 30 days of infection, with high mortality rate up to 30 days (79 to 100%); clones C2, C5 and C7 presented lower levels of parasitemia, with low mortality rates (7.6 to 23%). Isoenzymic patterns, characteristic of zymodeme 1, (Z1) were similar for the parental strain and its seven clones. Results point to a phenotypic homogeneity of the clones isolated from the Colombian strain and suggest the predominance of a principal clone, responsible for the biological behavior of the parental strain and clones. |
format |
Article in Journal/Newspaper |
author |
Camandaroba Edson Luiz Paes Campos Rozália Figueira Magalhães Juracy Barbosa Andrade Sonia G. |
author_facet |
Camandaroba Edson Luiz Paes Campos Rozália Figueira Magalhães Juracy Barbosa Andrade Sonia G. |
author_sort |
Camandaroba Edson Luiz Paes |
title |
Clonal structure of Trypanosoma cruzi Colombian strain (biodeme Type III): biological, isoenzymic and histopathological analysis of seven isolated clones |
title_short |
Clonal structure of Trypanosoma cruzi Colombian strain (biodeme Type III): biological, isoenzymic and histopathological analysis of seven isolated clones |
title_full |
Clonal structure of Trypanosoma cruzi Colombian strain (biodeme Type III): biological, isoenzymic and histopathological analysis of seven isolated clones |
title_fullStr |
Clonal structure of Trypanosoma cruzi Colombian strain (biodeme Type III): biological, isoenzymic and histopathological analysis of seven isolated clones |
title_full_unstemmed |
Clonal structure of Trypanosoma cruzi Colombian strain (biodeme Type III): biological, isoenzymic and histopathological analysis of seven isolated clones |
title_sort |
clonal structure of trypanosoma cruzi colombian strain (biodeme type iii): biological, isoenzymic and histopathological analysis of seven isolated clones |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2001 |
url |
https://doaj.org/article/00c742bd7afb44a59008f615d38358e6 |
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Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 34, Iss 2, Pp 151-157 (2001) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822001000200001 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/00c742bd7afb44a59008f615d38358e6 |
_version_ |
1766338229749940224 |